Product Code | Description | Product Size | Quantity |
CM0777B | Raka-Ray Medium 500g | – | 500g |
Raka-Ray Medium 500g
Brand:
Thermo ScientificTM OxoidTM
- Oxoid Raka-Ray Agar Base is a medium for the isolation of lactic acid bacteria in beer and brewing processes.
- Requires supplementation with phenylethanol, sorbitan mono-oleate, and cycloheximide Part No. SR0222C.
Raka-Ray Agar Base, Oxoid Composition
Typical Formula* | gm/litre |
Yeast extract | 5.0 |
Tryptone | 20.0 |
Liver concentrate | 1.0 |
Maltose | 10.0 |
Fructose | 5.0 |
Glucose | 5.0 |
Betaine HCL | 2.0 |
Diammonium hydrogen-citrate | 2.0 |
L-Aspartic acid† | 2.5 |
L-Glutamic acid†† | 2.5 |
Magnesium sulphate. 7H2O | 2.0 |
Manganese (II) sulphate. 4H2O | 0.66 |
Potassium phosphate | 2.0 |
N-Acetyl glucosamine | 0.5 |
Agar | 17.0 |
pH 5.4 ± 0.2 @ 25°C |
Raka-Ray Agar, Oxoid Preparation:
Suspend 77.1g in 1 litre of distilled water. Add 10ml of sorbitan mono-oleate and 7ml of 0.1% Cycloheximide Solution (SR0222). Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 3g of phenylethanol. Pour into sterile Petri dishes or distribute into 4ml volumes held at 55°C if the overlay technique is to be used.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Although the concentration of cycloheximide in the medium is below toxic levels, precautions should be observed as detailed under HAZARDS section.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |