Brand:
Thermo ScientificTM OxoidTM
The Oxoid Tryptone Bile Agar is used for the detection and enumeration of E.coli biotype 1 in foods.
Tryptone Bile Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 20.0 |
| Bile salts No.3 | 1.5 |
| Agar | 15.0 |
| pH 7.2 ± 0.2 @ 25°C | |
Tryptone Bile Agar, Oxoid Preparation:
Suspend 36.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour 12-15ml of the medium into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.