Brand:
Thermo ScientificTM OxoidTM
The Oxoid Tryptone Bile Agar is used for the detection and enumeration of E.coli biotype 1 in foods.
Tryptone Bile Agar, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Tryptone |
20.0
|
| Bile salts No.3 |
1.5
|
| Agar |
15.0
|
| pH 7.2 ± 0.2 @ 25°C |
|
Tryptone Bile Agar, Oxoid Preparation:
Suspend 36.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour 12-15ml of the medium into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.