Brand:
Thermo ScientificTM OxoidTM
Oxoid Vogel-Johnson Agar is used for the detection of Staphylococcus aureus from foods and clinical specimens.
Vogel-Johnson Agar, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Tryptone |
10.0
|
| Yeast extract |
5.0
|
| Mannitol |
10.0
|
| Dipotassium phosphate |
5.0
|
| Lithium chloride |
5.0
|
| Glycine |
10.0
|
| Phenol red |
0.025
|
| Agar |
16.0
|
| pH 7.2± 0.2 @ 25°C |
|
Vogel-Johnson Agar, Oxoid Preparation:
Suspend 61g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5.7ml of sterile 3.5% potassium tellurite solution SR0030 (equivalent to 20ml of 1% potassium tellurite).
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
All presumptive Staphylococcus aureus colonies should be confirmed with further tests. Do not heat the medium after the addition of potassium tellurite.