Brand:
Thermo ScientificTM OxoidTM
Oxoid Vogel-Johnson Agar is used for the detection of Staphylococcus aureus from foods and clinical specimens.
Vogel-Johnson Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 10.0 |
| Yeast extract | 5.0 |
| Mannitol | 10.0 |
| Dipotassium phosphate | 5.0 |
| Lithium chloride | 5.0 |
| Glycine | 10.0 |
| Phenol red | 0.025 |
| Agar | 16.0 |
| pH 7.2± 0.2 @ 25°C | |
Vogel-Johnson Agar, Oxoid Preparation:
Suspend 61g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5.7ml of sterile 3.5% potassium tellurite solution SR0030 (equivalent to 20ml of 1% potassium tellurite).
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
All presumptive Staphylococcus aureus colonies should be confirmed with further tests. Do not heat the medium after the addition of potassium tellurite.