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Lambda DNA – 250 ug
RM415.00Brand:
New England Biolabs
- Commonly used DNA substrate
- Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). Lambda DNA is 48,502 base pairs in length
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pBR322 Vector
RM450.00 – RM1,610.00Brand:
New England Biolabs
- pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1).
- The molecule is a double-stranded circle 4,361* base pairs in length (2).
- pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
- The molecular weight is 2.83 x 106 daltons.
*Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported.
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pUC19 Vector
RM440.00 – RM1,600.00Brand:
New England Biolabs
- pUC19 is a commonly used cloning vector that conveys Tet and Amp resistance.
- The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
- pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases.
- NEB offers a selection of common cloning plasmids and DNAs for use as substrates.