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EnGen Mutation Detection Kit – 25 rxns

RM1,073.00
Brand:

New England Biolabs

  • Simple protocol for detecting targeting efficiency in genome editing experiments
  • Optimized reagents for performing robust T7 Endonuclease-based detection of genome editing events
  • Q5® Hot Start High Fidelity 2X Master Mix included for robust amplification, high fidelity and convenience
  • Rapid protocol requires no cleanup between PCR and digestion
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Gibson Assembly Master Mix

RM853.00 – RM3,279.00
Brand:

New England Biolabs

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

  • Assembly and transformation in just under two hours
  • Flexible sequence design (scar-less cloning)
  • No PCR clean-up step required
  • High transformation efficiencies for inserts up to 20 kb
  • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
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NEB PCR Cloning Kit (Without Competent Cells) – 20 rxns

RM859.00
Brand:

New England Biolabs

Easy cloning of all PCR products, including blunt and TA ends

  • In vitro transcription with both SP6 and T7 promoters
  • BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules)
  • Fast cloning experiments with 5-minute ligation step
  • Simplified screening with low/no colony background and no blue/white selection required
  • Save time by eliminating purification steps
  • Flanking restriction sites available for easy subcloning, including choice of two single digest options
  • Provided analysis primers allow for downstream colony PCR screening or sequencing
  • Ready-to-use kit components include 1 kb control amplicon, linearized cloning vector and single-use competent E. coli
  • Longer shelf life (12 months), as compared to some commercially available products
  • Also available with competent cells
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NEBuilder HiFi DNA Assembly Master Mix

RM829.00 – RM3,183.00
Brand:

New England Biolabs

NEBuilder® HiFi DNA Assembly Master Mix allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. Visit NEBuilderHifi.com for more information.

  • Also available with competent cells at a discounted price.
  • Enjoy less screening/re-sequencing of constructs, with virtually error-free, high-fidelity assembly
  • Join DNA fragments together more efficiently, even with larger fragments or low DNA inputs
  • Use NEBuilder HiFi in successive rounds of assembly, because it removes 5´ and 3´ end mismatches. (Save time by avoiding time-consuming PCR amplification steps.)
  • Bridge two ds-fragments with a synthetic ss-DNA oligo for simple and fast construction (e.g., linker insertion or gRNA library)
  • Use with lower DNA input requirements
  • No licensing fee requirements from NEB for NEBuilder products
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Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) – 10 rxns

RM719.00
Brand:

New England Biolabs

The Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

  • Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
  • Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
  • Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
  • Hot start polymerase enables room temperature reaction setup
  • DpnI background reduction permits a wide range of starting template concentrations
  • Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
  • Easy-to-use PCR master mix and unique multi-enzyme KLD mix offer convenience and quality
  • Rapid and direct treatment step proceeds at room temperature in 5 minutes
  • Allows the use of any chemically-competent E. coli cells suitable for cloning
  • Use NEBaseChanger to generate primer sequences and an annealing temperature
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