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NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing

RM4,660.00
Brand:

New England Biolabs

The NEBNext DNA repair, end repair and ligation reagents recommended in Ligation library preparation are now available in the same product, at volumes designed for use in several protocols alongside Oxford Nanopore Technologies SQK-LSK109 and SQK-LSK110.

  • Component volumes tailored for use with many SQK-LSK109 and SQK-LSK110 workflows
  • Simplified ordering and inventory management
  • Compatible with all devices: MinION®, GridION®, PromethION™, Flongle®
  • No more waste – no unnecessary buffers or excess reagents

NEBNext® Library Quant Kit for Illumina®

RM570.00RM2,225.00
Brand:

New England Biolabs

Accurate quantitation of next-generation sequencing (NGS) libraries is essential for maximizing data output and quality from each sequencing run. For Illumina® sequencing specifically, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. Further, amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced. The NEBNext® Library Quant Kit has been optimized to provide substantial performance and workflow improvements to qPCR-based library quantitation.

  • Be confident in your quant values, with more accurate and reproducible results than other methods and kits.
  • Enjoy the flexibility to use either four or six standards (included).
  • Simplify your reaction setup with fewer pipetting steps and a single extension time for all libraries.
  • Use with all your libraries, regardless of insert size, GC content and preparation method.

Additional dilution buffer can be purchased separately.

NEBNext® Magnesium RNA Fragmentation Module

RM300.00
Brand:

New England Biolabs

Fragmentation of RNA can be performed by enzymes, heat alone, or exposure to divalent cations at elevated temperature. This kit provides highly efficient and tunable RNA fragmentation by incubation with Magnesium ions at 94˚C.

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

RM3,360.00
Brand:

New England Biolabs

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • 96-well plate format with pierceable foil seal

NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2)

RM2,775.00RM9,830.00
Brand:

New England Biolabs

For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification. This second set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • Increased sample identification specificity
  • Enables complete filtering of index-hopped reads
  • Highly efficient adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Combine with sets 1, 3, and 4 for 384 indices

NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 3)

RM2,775.00
Brand:

New England Biolabs

For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification. This third set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • Enables complete filtering of index-hopped reads
  • Highly efficient adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Combine with sets 1, 2, and 4 for 384 barcodes

NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 4)

RM2,775.00
Brand:

New England Biolabs

For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification.  This fourth set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • Enables complete filtering of index-hopped reads
  • Highly efficient adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Combine with sets 1, 2, and 3 for 384 barcodes

NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)

RM2,775.00
Brand:

New England Biolabs

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • Allows complete filtering of index-hopped reads
  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity
  • Combine with sets 2, 3, and 4 for 384 indices

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

RM2,395.00
Brand:

New England Biolabs

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)

RM2,395.00
Brand:

New England Biolabs

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

RM595.00RM2,015.00
Brand:

New England Biolabs

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) (NEB #7335S/L) includes 12 indices.

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

RM595.00RM2,015.00
Brand:

New England Biolabs

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) (NEB #7335S) includes 12 indices.

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

RM595.00RM2,015.00
Brand:

New England Biolabs

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) (NEB #7335) includes 12 indices.

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

RM595.00RM2,015.00
Brand:

New England Biolabs

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) ( NEB #7335) includes 12 indices.

NEBNext® Poly(A) mRNA Magnetic Isolation Module

RM385.00RM1,300.00
Brand:

New England Biolabs

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.

NEBNext® RNA Depletion Core Reagent Set

RM1,330.00RM4,680.00
Brand:

New England Biolabs

Introducing a new solution for customized depletion of abundant RNAs.

In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.

The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.

  • Compatible with a broad range of input amounts: 10 ng – 1µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available with RNAClean® beads.

NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads

RM1,370.00RM4,875.00
Brand:

New England Biolabs

Introducing a new solution for customized depletion of abundant RNAs.

In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.

The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.

  • Compatible with a broad range of input amounts: 10 ng – 1µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available without RNAClean® beads.

NEBNext® RNA First Strand Synthesis Module

RM875.00RM2,670.00
Brand:

New England Biolabs

This module is optimized for use in the original Ultra™ workflow, for Illumina®-compatible RNA library construction. The original Ultra workflow enables library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol.

However, we now recommend use of the more recent NEBNext Ultra II Directional RNA and RNA (non-directional) workflows, which have been further optimized to provide increased yields of high quality libraries from a broader range of input amounts, and a NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) is available.

NEBNext® rRNA Depletion Kit (Bacteria)

RM1,545.00RM5,460.00
Brand:

New England Biolabs

Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse – efficient and specific removal of bacterial rRNA – is necessary.

The NEBNext® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).

  • Efficient, specific depletion of bacterial rRNA (5S, 16S, 23S)
  • Compatible with both gram-positive and gram-negative organisms
  • Effective with monocultures and mix of bacterial species (e.g., metatranscriptome)
  • Compatible with a broad range of input amounts: 10 ng – 1 µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available with RNAClean® beads.

NEBNext® rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads

RM1,585.00RM5,685.00
Brand:

New England Biolabs

Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse – efficient and specific removal of bacterial rRNA – is necessary.

The NEBNext® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).

For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt® RNAClean® XP beads from Beckman Coulter).

  • Efficient, specific depletion of bacterial rRNA (5S, 16S, 23S)
  • Compatible with both gram-positive and gram-negative organisms
  • Effective with monocultures and mix of bacterial species (e.g., metatranscriptome)
  • Compatible with a broad range of input amounts: 10 ng – 1 µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available without RNAClean® XP beads.

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

RM1,760.00RM6,240.00
Brand:

New England Biolabs

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples, but this can be especially challenging with low quality RNA (e.g. FFPE RNA) and with low input amounts. The NEBNext rRNA Depletion kit employs the efficient RNase H method, as well as complete probe tiling of rRNA, thereby ensuring that even degraded rRNA is hybridized and subsequently removed.

  • Suitable for low-quality (e.g. FFPE) or high-quality RNA
  • Compatible with a broad range of input amounts: 10 ng – 1 μg
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

RM1,810.00RM6,500.00
Brand:

New England Biolabs

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method (1), (2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt® RNAClean® XP beads from Beckman Coulter).

NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)

RM1,545.00RM5,460.00
Brand:

New England Biolabs

This product provides superior performance compared to the original E6310 kit. Note that RNA Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext rRNA Depletion Kit v2 with Sample Purification Beads (NEB #E7405). 

The abundance of ribosomal RNAs (rRNAs), constituting 80–90% of total RNA, necessitates removal upstream of next generation sequencing and other applications. This second generation rRNA depletion kit incorporates reagent, probe and protocol improvements, resulting in superior depletion performance. The efficient RNAse-H-based workflow, and close spacing of probes, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.

  • Superior depletion of rRNA from human, mouse and rat RNA.
  • Depletion of cytoplasmic (5S, 5.8S, 18S, 28S, human ITS, ETS) and mitochondrial (12S and 16S) rRNA
  • Compatible with a broad range of input amounts: 10 ng – 1 µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads

RM1,585.00RM5,685.00
Brand:

New England Biolabs

The abundance of ribosomal RNAs (rRNAs), constituting 80–90% of total RNA, necessitates removal upstream of next generation sequencing and other applications. This second generation rRNA depletion kit incorporates reagent, probe and protocol improvements, resulting in superior depletion performance. The efficient RNAse-H-based workflow, and close spacing of probes, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.

For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt® RNAClean® XP beads from Beckman Coulter).

  • Superior depletion of rRNA from human, mouse and rat RNA
  • Depletion of cytoplasmic (5S, 5.8S, 18S, 28S, human ITS, ETS) and mitochondrial (12S and 16S) rRNA
  • Compatible with a broad range of input amounts: 10 ng – 1 µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time