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EnGen sgRNA Synthesis Kit, S. pyogenes – 20 rxns

RM2,113.00
Brand:

New England Biolabs

The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.

HiScribe SP6 RNA Synthesis Kit – 50 rxns

RM1,423.00
Brand:

New England Biolabs

  • Generate over 80 μg RNA per reaction
  • Enables full substitution of NTPs for labeling and incorporation of modified bases
  • Template removal and mRNA purification reagents included
  • Linearized control template included for verification of RNA synthesis

HiScribe T7 ARCA mRNA Kit – 20 rxns

RM1,639.00
Brand:

New England Biolabs

  • Generate up to 20 μg of capped mRNA per reaction
  • mRNA capping, DNA removal, mRNA tailing and purification complete in 2 hrs. Competitive products have more pipetting steps and separate components.
  • Enables partial incorporation of 5mCTP, Pseudo-UTP and other modified CTP and UTP
  • Ultra-high quality components ensure mRNA integrity
  • ARCA-based capping in correct orientation ensures high translation efficiency
  • Template removal and mRNA purification reagents included
  • HiScribe™ kits contain twice the number of reactions as competitive products
  • Also available with tailing reagents

HiScribe T7 ARCA mRNA Kit (with Tailing) – 20 rxns

RM1,913.00
Brand:

New England Biolabs

  • Generate up to 25 μg of capped and tailed mRNA per reaction
  • mRNA capping, DNA removal, mRNA tailing and purification complete in 2 hours. Competitive products have more pipetting steps and separate components.
  • Enables partial incorporation of 5mCTP, Pseudo-UTP and other modified CTP and UTP
  • Ultra high-quality components ensure mRNA integrity
  • ARCA-based capping in correct orientation ensures high translation efficiency
  • Template removal and mRNA purification reagents included
  • HiScribe kits contain twice the number of reactions as competitive products
  • Also available without tailing reagents (NEB #E2065)

HiScribe T7 High Yield RNA Synthesis Kit – 50 rxns

RM1,169.00
Brand:

New England Biolabs

  • Up to 180 μg of RNA per reaction from 1 μg of control template
  • Enables full substitution of NTPs for labeling and incorporation of modified bases
  • Linearized control template included for verification of RNA synthesis

HiScribe T7 Quick High Yield RNA Synthesis Kit – 50 rxns

RM1,423.00
Brand:

New England Biolabs

  • Up to 180 μg of RNA generated per reaction, from 1 μg of control template
  • Master mix format reduces pipetting steps while still enabling partial substitution of NTPs for labeling and incorporation of modified bases
  • Template removal and RNA purification reagents included
  • Linearized control template included for verification of RNA synthesis

Magnetic mRNA Isolation Kit

RM1,669.00
Brand:

New England Biolabs

  • Designed to isolate intact poly(A)+ RNA from cells and tissue.
  • Automation friendly.
  • Reusable up to 3 times.

polyA Spin mRNA Isolation Kit

RM1,173.00
Brand:

New England Biolabs

This kit contains an affinity matrix used for the isolation of mRNA containing polyadenylic (poly A) regions (1) from purified total RNA. This matrix consists of oligo (dT)25 covalently coupled to a cross-linked cellulose bead. This product is guaranteed for twenty-four months when stored at 4°C in the supplied storage buffer.

  • A rapid and convenient alternative to traditional column chromatography for the isolation of full-length poly(A)+ eukaryotic messenger (mRNA) from samples of Total RNA.
  • Shorter purification time: Prepare full-length mRNA in as little as 40 minutes.
  • Maintains Resolution: When compared to a 100 mg column of high quality oligo (dT) cellulose in 1.0 mg total RNA sample range.

Random Primer 6

RM529.00
Brand:

New England Biolabs

  • Random Primer 6 is a mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA.
  • Ensures the presence of virtually all sequence combination of hexamer primers.
  • Used to screen gene libraries.
  • Used to probe northern and southern blots.
  • Used for in situ hybridizations.
  • Sequence: 5′ d(N6 ) 3′ [N=A,C,G,T].

Random Primer 9

RM529.00
Brand:

New England Biolabs

A mixture of random nonanucleotides used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). The primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is “oligo-labelled” by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N9) primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity (1,2).

Oligolabeling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).

Sequence

5′ d(N9) 3′ [N = A,C,G,T].

Random Primer Mix

RM529.00
Brand:

New England Biolabs

  • Random priming is widely used in first strand cDNA synthesis.
  • Random Primer Mix is a ready-to-use optimized mixture of hexamers and anchored-dT primer (dT23VN).
  • A mixture of hexamers and anchored-dT primer provides even and consistent coverage of the RNA template population across a wide range of RNA template concentration. In contrast, traditional random priming by hexamer has poor coverage of 3´ end of RNA templates (1,2,3).