Pseudomonas Agar Base 500g

Selectively isolate Pseudomonas species from a range of samples with Thermo Scientific™ Oxoid™ Pseudomonas Agar Base (Dehydrated). The medium is modification of King’s A Medium and contains magnesium chloride and potassium sulphate for improved pigment production and recovery of Pseudomonas aeruginosa while strongly suppressing Klebsiella, Proteus and Providencia spp.

Pseudomonas Agar Base (Dehydrated), Oxoid Composition

Agar Preparation:
To Prepare the Agar Base:
Suspend 24.2g of the agar base, in 500ml of distilled water. Add 5ml of glycerol. Bring to the boil to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes. Allow the medium to cool to 50°C.

To Prepare Pseudomonas CN Agar:
To 500ml of agar base cooled to 50°C add the contents of 1 vial of Pseudomonas CN Supplement (SR0102) rehydrated as directed. Mix well and pour into sterile Petri dishes.

To Prepare Pseudomonas CFC Agar:
To 500ml of agar base cooled to 50°C add the contents of 1 vial of Pseudomonas CFC Supplement (SR0103) rehydrated as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Fresh media should be prepared as required. Molten agar should not be kept longer than 4 hours. Medium should not be stored and remelted. If swarming colonies of Proteus species are a problem in food samples then the incubation temperature can be lowered to 20°C for a period of 3-5 days. Chilled foods may carry a wide range of pseudomonads and the colonies on CFC Medium, incubated at lower temperatures, may be Pseudomonas fluorescens or Pseudomonas putida as well as Pseudomonas aeruginosa. Aeromonas species will also appear as pink/brown colonies, particularly from fish products.