Brilliant Green Bile 2% Broth 500g, Oxoid

Oxoid Brilliant Green Bile Broth 2% is used for detection or confirmation of presence of members of coli-aerogenes group.

• Recommended for 44°C confirmatory test for Escherichia coli

Brilliant Green Bile Broth 2%, Oxoid Composition

Brilliant Green Bile Broth 2%, Oxoid Preparation:
Dissolve 40g in 1 litre of distilled water. Mix well, distribute into containers fitted with Durham’s tubes and sterilise by autoclaving at 121°C for 15 minutes.

Do not autoclave double stregnth medium. The alternative procedure is to heat the dissolved broth at 100°C for 30 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared tubes of broth at 2-8°C.

Precautions
Do not autoclave double-strength broth.
Gram-positive sporing organisms may produce gas if the bile/brilliant green inhibition is attenuated by food material.