Brand:
Thermo ScientificTM OxoidTM
- Oxoid PALCAM Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
- Also available PALCAM Selective Supplement, Part No. SR0150E or SR0150B.
PALCAM Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Columbia Blood Agar Base |
39.0
|
| Yeast extract |
3.0
|
| Glucose |
0.5
|
| Aesculin |
0.8
|
| Ferric ammonium citrate |
0.5
|
| Mannitol |
10.0
|
| Phenol red |
0.08
|
| Lithium chloride |
15.0
|
| pH 7.2 ± 0.2 @ 25°C |
|
PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.
The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.
Precautions
Acriflavine hydrochloride is activated by light which may cause it to become inhibitory to Listeria growth.