Nucleic Acid Purification

• Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification
• Reaction complete in 4 minutes
• Thermolabile formulation can be heat inactivated in 1 minute at 80°C
• PCR product can be used directly in downstream applications
• Compatible with commonly-used reaction buffers

Quickly and easily purify DNA from agarose gels with high yields.

• Elute in as little as 6 μl.
• Prevent buffer retention and salt carry-over with optimized column design.
• Save time with fast, user-friendly protocols
• No need to monitor pH or add isopropanol.
• Buffers and columns available separately.
• Significantly less plastic used when compared with other kits.
• Responsibly-sourced and recyclable packaging.

** Tips for using Monarch DNA Gel Extraction kit

Quickly and easily purify high-quality DNA from PCR and other enzymatic reactions.

• Elute in as little as 6 μl.
• Prevent buffer retention and salt carry-over with optimized column design.
• Save time with fast, user-friendly protocols.
• No need to monitor pH.
• Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments.
• Buffers and columns available separately.
• Significantly less plastic used when compared with other kits.
• Responsibly-sourced and recyclable packaging.

** Tips for using Monarch PCR and DNA Cleanup Kit

The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of up to 20 μg of high quality plasmid DNA.

• Elute in as little as 30 μl
• Prevent buffer retention and salt carry-over with optimized column design
• Reduce hands on time with faster protocols and less spin time
• Monitor completion of certain steps using colored buffer system
• No need to add RNase before starting
• Easily label columns using tab and frosted surfaces
• Buffers and columns available separately
• Significantly less plastic used when compared with other kits
• Responsibly-sourced and recyclable packaging
• No hazardous materials fees

** Tips for using Monarch Plasmid Miniprep Kit

The Monarch RNA Cleanup Kit (10 µg) enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

• Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
• Elute in ≥ 6 µl for concentrated RNA
• 70-100% RNA recovery, even with inputs < 20 ng
• Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
• Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
• Simplified workflow with a single wash buffer
• Unique column design prevents buffer carryover and elution of silica particulates
• Columns and buffers are available separately
• Purified RNA is ready for use in a wide variety of downstream applications

** Tips for using Monarch RNA Cleanup Kit

The Monarch RNA Cleanup Kit (500 µg), NEB enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.

• Ideal for purification of synthesized RNA following high-yield in vitro transcription reactions (alternative to MEGAclear™)
• Optimized for the cleanup of RNA after enzymatic treatments including DNase I, Proteinase K, labeling and capping
• Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
• Elute in ≥ 50 µl for concentrated RNA 70-100% RNA recovery
• Unique column design prevents buffer carryover and elution of silica particulates
• Simplified workflow with a single wash buffer
• Columns and buffers are available separately
• Purified RNA is ready for use in a wide variety of downstream applications, including microinjection and transfection.

** Tips for using Monarch RNA Cleanup Kit

Quickly and easily purify high quality, high molecular weight gDNA from multiple sample types, all with one kit!

• For use with a broad range of sample types, such as cells, blood, and tissues (including fatty and fibrous tissues)
• Also works with tough to lyse samples (e.g., bacteria, yeast)
• Suitable for clinically-relevant samples (e.g., saliva, cheek)
• Obtain excellent yields of highly-pure DNA
• Extremely low residual RNA contamination (typically <1%)
• Isolates high molecular weight gDNA (peak size typically ≥ 50 kb)
• Use DNA directly in downstream applications, including PCR, qPCR and NGS
• Fast, user-friendly protocols with short lysis times and minimal hands-on time
• Can also be used to clean up previously extracted gDNA
• Includes RNase A
• Kit components available separately

** Tips on how to maximize DNA yield using Monarch Genomic DNA Purification kit

• For the extraction of HMW DNA from cells and blood
• Isolate DNA into the Mb size range
• Easily tune the size of isolated DNA by adjusting the agitation speed during lysis
• Fast, user-friendly workflows (30 minutes for cells, 60 minutes for blood)
• Obtain best-in-class yields of highly-pure and intact DNA
• Effective RNA removal
• Rapid and efficient elution
• Includes RNase A and Proteinase K
• Ideal for long-read sequencing applications

• For the extraction of HMW DNA from a variety of tissues, bacteria and other samples (yeast, insect, amphibian)
• Isolate DNA into the Mb size range from soft organ tissues and bacteria
• For some samples, the size of isolated DNA can be tuned by adjusting the agitation speed during lysis
• Fast, user-friendly workflows (90 minutes for tissues and bacteria)
• Obtain excellent yields of highly-pure and intact DNA
• Rapid and efficient elution
• Ideal for long-read sequencing applications
• Includes RNase A and Proteinase K, as well as microtube pestles for convenient sample homogenization

The Monarch RNA Cleanup Kit (50 µg), NEB enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

• Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labelling, capping or in vitro transcription (IVT)
• Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
• Elute in ≥ 20 µl for concentrated RNA
• 70-100% RNA recovery
• Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
• Simplified workflow with a single wash buffer
• Unique column design prevents buffer carryover and elution of silica particulates
• Columns and buffers are available separately
• Purified RNA is ready for use in a wide variety of downstream applications, including transfection

** Tips for using Monarch RNA Cleanup Kit

Description

Synonyms: RNA preservation solution, RNA protection solution, RNA protector, RNA stabilizer, RNA storage solution, RNA transportation solution

RNAlater® is an RNA stabilization solution that is designed to quickly permeate and stabilize RNA in tissues and cells after collection. It allows for long-term storage of samples at different temperatures, including room temperature, without compromising RNA integrity. It preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.