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The method corresponds to DIN 38405-13 and is analogous to ISO 6703, EPA 335.2, APHA 4500-CN- E, and ASTM D2036-09 D.

This Spectroquant® Cyanide Reagent Test allows the accurate quantification of the free and readily liberated cyanide content in aqueous samples.

Method applied: Cyanide ions react with a chlorinating agent to form cyanogen chloride. In a follow-up reaction with 1,3-dimethylbarbituric acid in the presence of pyridine a violet dye (König reaction) is formed which is determined photometrically (only free cyanide. For readily liberated cyanide samples must be decomposed by digestion).

The Spectroquant® Reagent Test Kits contain highly stable, ready-to-use reagent mixtures to perform the analysis according to the procedure described in the accompanying instruction leaflet.

All our Cell and Reagent Test Kits are equipped with the unique Live ID which allows seamless method recognition and contains essential information such as lot number, expiry date, and automatic calibration updates.

Description

CAS number: 110-82-7
Chemical Formula: C₆H₁₂
Molar Mass: 84.16 g/mol
Synonyms: Hexahydrobenzene, Hexamethylene, Naphthene

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter

Description

CAS number: 66-81-9
Chemical Formula: C15H23NO4
Molar Mass: 281.35 g/mol
Synonyms: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide, Actidione, Naramycin A

Application

In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment. It has been used to inhibit translation in mammalian cells and suppress fungal growth.

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Description

CAS number: 9007-43-6

Application

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c.

Biochem/physiol Actions

The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.