Potassium Nitrate for Analysis EMSURE® ISO,Reag. Ph Eur
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Merck
Description
CAS number: 7757-79-1
Chemical Formula: KNO₃
Molar Mass: 101.10 g/mol
Synonyms: Nitric acid potassium salt
Specifications:
| Assay (alkalimetric) | ≥ 99.0 % |
| pH-value (5 %; water) | 5.0 – 7.5 |
| Chloride (Cl) | ≤ 0.001 % |
| Iodate (IO₃) | ≤ 0.0005 % |
| Nitrite (NO₂) | ≤ 0.001 % |
| Phosphate (PO₄) | ≤ 0.0005 % |
| Sulfate (SO₄) | ≤ 0.003 % |
| Heavy metals (as Pb) | ≤ 0.0005 % |
| Ca (Calcium) | ≤ 0.001 % |
| Cu (Copper) | ≤ 0.0001 % |
| Fe (Iron) | ≤ 0.0003 % |
| Mg (Magnesium) | ≤ 0.0015 % |
| Na (Sodium) | ≤ 0.02 % |
| NH₄ (Ammonium) | ≤ 0.001 % |
| Pb (Lead) | ≤ 0.0001 % |
| Corresponds to ISO,Reag. Ph Eur | |
ProteoExtract® Native Membrane Protein Extraction Kit
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Merck
Description
A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications.
Note: 1 kit is sufficient for up to 20 sample extractions.
ProteoExtract® Subcellular Proteome Extraction Kit
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Merck
Description
Fast and reproducible extraction of subcellular proteomes from mammalian cells
ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells. The S-PEK takes advantage of the different solubilities of certain subcellular compartments in the four selected reagents. In the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. Cells or the parts of the cells remain attached to the plate during sequential extraction of subcellular compartments, until the appropriate extraction reagent is used. Thus, the early destruction of the cellular structure by enzymatic or mechanical detachment of cells from the tissue culture plate and any mixing of different subcellular compartments is prevented. For suspension-grown cells, extraction starts with gentle sedimentation and washing of the cells. The stepwise extraction delivers four distinct protein fractions from one sample:
– Cytosolic fraction (F1)
– Membrane/organelle protein fraction (F2)
– Nucleic protein fraction (F3)
– Cytoskeletal fraction (F4)
Proteins are obtained in the native state making the S-PEK suitable for many downstream applications such as 1D and 2D gel electrophoresis, immunoblotting, enzyme activity assays, and protein microarrays.
Sample size: 3-5×106 or 25-50 mg tissue.
ProteoExtract® Transmembrane Protein Extraction Kit
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Merck
Description
The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
SignalBoost™ Immunoreaction Enhancer Kit
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Merck
Description
SignalBoost™ Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies. It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required.
• Increases signal: minimizes background resulting in high signal to noise ratios
• Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems
• Convenient: ready-to-use format, no dilution necessary
Silica gel 60 (0.040-0.063 mm) for Column Chromatography (Merck)
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Merck
Description
Silica gel is a type of stationary phase material used in column chromatography, a separation technique that separates components of a mixture based on their chemical properties. The silica gel 60 offered by Merck has a particle size of 0.040-0.063 mm, which is equivalent to 230-400 mesh according to the ASTM standard. This makes it suitable for use in column chromatography applications where a fine, uniform particle size is required for efficient separation of target compounds. Merck’s Silica Gel 60 is a high-quality product that is widely used in research and industrial laboratories for various chromatography applications.
Silica gel 60 (0.063-0.200 mm) for column chromatography (70-230 mesh ASTM)-Merck
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Merck
Description
Cas Number : 7631-86-9
Molecular mass : 60.08 g/mol
Chemical formula : SiO₂
Silica gel 60 (0.063-0.200 mm) for column chromatography (70-230 mesh ASTM). CAS No. 7631-86-9, EC Number 231-545-4.
Silver Nitrate for Analysis EMSURE® ACS,ISO,Reag. Ph Eur (Merck)
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Merck
Description
CAS Number: 7761-88-8
Chemical Formula: AgNO₃
Molecular Weight: 169.87 g/mol
Quality Level: MQ300
Silver Nitrate for analysis EMSURE® ACS,ISO,Reag. Ph Eur (Merck)
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Merck
CAS no.: 7761-88-8
Grade: For analysis EMSURE® ACS,ISO,Reag. Ph Eur
Chemical Formula: AgNO₃
Synonym: –
Quality level: MQ300
Product specification and SDS for Silver Nitrate for analysis EMSURE®.
Sodium carbonate anhydrous for analysis EMSURE® ISO (Merck)
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Merck
Description
CAS number: 497-19-8
Chemical Formula: Na₂CO₃
Molar Mass: 105.99 g/mol
Synonyms: anhydrous soda
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for Sodium carbonate EMSURE® (Merck).
Sodium chloride for Analysis EMSURE® ACS,ISO,Reag. Ph Eur (Merck)
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Merck
Description
CAS number: 7647-14-5
Chemical Formula: NaCl
Molar Mass: 58.44 g/mol
Sodium sulfate anhydrous for analysis EMSURE® ACS,ISO,Reag. Ph Eur (Merck)
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Merck
Description
CAS number: 7757-82-6
Chemical Formula: Na₂SO₄
Molar Mass: 142.04 g/mol
Synonyms: –
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for Sodium sulfate EMSURE® (Merck).
tert-Butyl methyl ether for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 1634-04-4
Chemical Formula: (CH₃)₃COCH₃
Molar Mass: 88.15 g/mol
Synonyms: MTBE, MTB, 2-Methoxy-2-methylpropane, Methyl tert-butyl ether
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter
Tetrahydrofuran for analysis EMSURE® ACS,Reag. Ph Eur (Merck)
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Merck
Description
CAS number: 109-99-9
Chemical Formula: C₄H₈O
Molar Mass: 72.11 g/mol
Synonyms: THF, Tetramethylene oxide, Oxolane, Butylene oxide
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for Tetrahydrofuran EMSURE® (Merck).
Tetrahydrofuran for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 109-99-9
Chemical Formula: C₄H₈O
Molar Mass: 72.11 g/mol
Synonyms: THF, Tetramethylene oxide, Oxolane, Butylene oxide
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter
TLC Silica gel 60 F254 (5×10 cm) Merck
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Merck
Description
For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 50 and measure 5×10 cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.
TLC Silica gel 60 F254, (20×20 cm) – Merck
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Merck
Description
For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 25 and measure 20x20cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.
TLC Silica gel 60 F254, (20×20 cm) – Merck
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Merck
Description
For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 25 and measure 20x20cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.
Toluene for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 108-88-3
Chemical Formula: C₆H₅CH₃
Molar Mass: 92.14 g/mol
Synonyms: Toluol, Methylbenzene
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter.
Trichloroacetic acid for Analysis EMSURE® ACS,Reag. Ph Eur (Merck)
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Merck
Description
CAS number: 76-03-9
Chemical Formula: CCl₃COOH
Molar Mass: 163.38 g/mol
Product Specification and MSDS for Trichloroacetic acid (Merck).
TWEEN® 20 Detergent (Merck)
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Merck
Description
CAS number: 9005-64-5
Synonyms: Polysorbate 20, Polyoxyethylene (20) sorbitan monolaurate, PEG(20)sorbitan monolaurate
Form: Pale yellow liquid
Non-ionic detergent used for immunoprecipitation and for the solubilization of membrane-bound proteins.
Product Specification and MSDS for TWEEN® 20 Detergent (Merck).
Water for chromatography (LC-MS Grade) LiChrosolv®
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Merck
Description
CAS number: 7732-18-5
Chemical Formula: H₂O
Molar Mass: 18.02 g/mol
Synonyms: Deionized water
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved.
YeastBuster™ Protein Extraction Reagent (for yeast cell lysis)
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Merck
Description
YeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
Zinc oxide for analysis EMSURE® ACS,Reag. Ph Eur
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Merck
Description
CAS number: 1314-13-2
Chemical Formula: ZnO
Molar Mass: 81.39 g/mol
Synonyms: –
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for Zinc oxide EMSURE® (Merck).
