Polyvinylpyrrolidone, K90 (Sigma-Aldrich)
RM989.00Brand:
Sigma-Aldrich
Description
CAS number: 9003-39-8
Chemical Formula: (C6H9NO)n
Synonym: PVP, Polyvidone, Povidone
Application
Polyvinylpyrrolidone (PVP), also known as K90 is a water-soluble polymer with good bio-stability. It is chemically stable, has low toxicity and is biocompatible. Hence, it is useful in a variety of applications such as cosmetics, tissue engineering, and biomedical engineering.
Other Notes
Polyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.
Potassium Iodide for analysis EMSURE® ISO,Reag. Ph Eur (Merck)
Price range: RM375.00 through RM2,886.00Brand:
Merck
CAS no.: 7681-11-0
Grade: For analysis EMSURE® ISO,Reag. Ph Eur
Chemical Formula: KI
Synonym: –
Quality level: MQ300
Product specification and SDS for Potassium Iodide for analysis EMSURE®.
Potassium Lactate 50%
RM372.00Brand:
Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Potassium Lactate is a solution for addition to culture media, Lysine Medium CM0191 for example.
Potassium Nitrate for Analysis EMSURE® ISO,Reag. Ph Eur
RM242.00Brand:
Merck
Description
CAS number: 7757-79-1
Chemical Formula: KNO₃
Molar Mass: 101.10 g/mol
Synonyms: Nitric acid potassium salt
Specifications:
| Assay (alkalimetric) | ≥ 99.0 % |
| pH-value (5 %; water) | 5.0 – 7.5 |
| Chloride (Cl) | ≤ 0.001 % |
| Iodate (IO₃) | ≤ 0.0005 % |
| Nitrite (NO₂) | ≤ 0.001 % |
| Phosphate (PO₄) | ≤ 0.0005 % |
| Sulfate (SO₄) | ≤ 0.003 % |
| Heavy metals (as Pb) | ≤ 0.0005 % |
| Ca (Calcium) | ≤ 0.001 % |
| Cu (Copper) | ≤ 0.0001 % |
| Fe (Iron) | ≤ 0.0003 % |
| Mg (Magnesium) | ≤ 0.0015 % |
| Na (Sodium) | ≤ 0.02 % |
| NH₄ (Ammonium) | ≤ 0.001 % |
| Pb (Lead) | ≤ 0.0001 % |
| Corresponds to ISO,Reag. Ph Eur | |
Potassium Tellurite 3.5%
RM319.00Brand:
Thermo ScientificTM OxoidTM
Oxoid Potassium Tellurite (3.5%) is a solution for addition to culture media.
Ordering Information:
Supplied 2mL/vial
Potato Dextrose Agar 500g, Oxoid
RM253.00Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate yeasts and molds from dairy and food products and prepare Aspergillus niger for the Harmonised Microbial Limit Tests from international pharmacopoeia with Thermo Scientific™ Oxoid™ Potato Dextrose Agar (Dehydrated). This medium is suitable for the detection and enumeration of heat resistant molds in thermally processed fruits and fruit products.
Pigment production is a critical part of the identification of the fungus. The agar used in the medium is carefully screened to ensure correct pigment production by fungi such as Fusaria spp.
Composition of Potato Dextrose Agar
| Typical Formula * | gm/litre |
| Potato Extract | 4.0 |
| Glucose | 20.0 |
| Agar | 15.0 |
| pH 5.6 ± 0.2 @ 25°C |
Preparation of Potato Dextrose Agar, Oxoid
Suspend 39g in 1 litre of purified water and heat up to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Ensure the solution is well mixed before pouring.
To suppress the growth of bacteria, you can acidify the medium to pH 3.5 by adding 1 ml of lactic acid 10% SR0021 to each sterilized medium at 50°C. Note that the medium must not be heated after the addition of acid as it will destroy the gelling properties and results in hydrolysis of agar.
Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Potato Dextrose Broth 500g
RM314.00Brand:
Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Potato Dextrose Broth is a general broth for yeasts and molds.
PowerPro 3 Amp
RM0.00Brand:
Cleaver Scientific
At 300V, 3000mA, 300W, the PowerPro 3AMP is designed for virtually all high current electrophoresis applications. The PowerPro 3AMP’s higher current output capability is perfect for electroblotting units with high-intensity plate electrodes, particularly Cleaver Scientific omniBLOT maxi, VS20 WAVE and semi dry blotting systems.
PowerPro 300
RM0.00Brand:
Cleaver Scientific
The PowerPro 300 is a versatile multi-function power supply suitable for a wide range of electrophoresis applications. At 300V, 700mA and 150W the PowerPro 300 has nearly twice the current and power of the market leading equivalent unit. Perfectly suited for use with Cleaver Scientific’s multiSUB range of horizontal electrophoresis systems and omniPAGE Mini vertical electrophoresis systems. The PowerPro 300 even has built in pre-set protocols from running each of Cleaver Scientific’s electrophoresis systems, and allows users to create and save protocols for their own systems.
The PowerPro 300 is the ideal power supply for:
- Routine horizontal electrophoresis using multiSUB Mini, Midi and Choice.
- Vertical Electrophoresis using omniPAGE Mini.
PowerPro 500
RM0.00Brand:
Cleaver Scientific
With a maximum 500V, 800mA and 300W voltage, current and power output, the PowerPro 500 is an excellent general-purpose power supply that fits the broadest range of electrophoresis applications, and can run as many as four units under a constant or programmable setting. It has the capacity to store up to 30 programmed files, each with 6 steps.
Pre-Supplemented DG18 (ISO) Agar
RM1,208.00Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated) while adhering to BS ISO 21527-2: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.
Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Casein Enzymatic digest | 5.0 |
| Glucose | 10.0 |
| Potassium dihydrogen phosphate | 1.0 |
| Magnesium sulphate | 0.5 |
| Dichloran (2,6-dichloro-4-nitroaniline) | 0.002 |
| Agar | 15 |
| Chloramphenicol | 0.1 |
| pH 5.6 ± 0.2 @ 25°C |
Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base, Oxoid Preparation:
Suspend 15.8g per 500ml (31.6g/l) of distilled water and heat to dissolve completely. Before autoclaving add 220g of Glycerol per 1 litre of medium. Heat the complete solution gently to aid its dissolution of the glycerol. This has been found to be a key procedure to follow to allow proper dissolution of the glycerol. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.
Where bacterial overgrowth occurs from certain foods (raw meats) chlortetracycline hydrochloride at 50mg/L (dissolved in water and filter sterilised) is recommended.
Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date.
Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).
Pre-supplemented DG18 (ISO) Agar (CM1151) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.
Pre-Supplemented DRBC (ISO) Agar
RM1,131.00Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated) while adhering to ISO 21527-1: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.
Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 5.0 |
| Glucose | 10.0 |
| Potassium dihydrogen phosphate | 1.0 |
| Magnesium sulphate | 0.5 |
| Dichloran | 0.002 |
| Rose-bengal | 0.025 |
| Agar | 15.0 |
| Chloramphenicol | 0.1 |
| pH 5.6 ± 0.2 @ 25°C |
Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Preparation:
Suspend 15.75g per 500ml (31.5g/l) of distilled water and heat to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes. It is advised to dispense the powdered medium into containers under an extraction hood due to the chloramphenicol in the medium base.
Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date
Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).
Pre-supplemented DRBC (ISO) Agar (CM1149) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.
Preston Campylobacter Selective Supplement 1 x 10 Vials
RM0.00Brand:
Thermo ScientificTM OxoidTM
This product has been discontinued. The recommended alternative is Modified Preston Campylobacter Supplement, SR0204E.
Oxoid Preston Campylobacter Selective Supplement is used for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.
- Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
- Each vial supplements 500mL of medium
Preston Campylobacter Selective Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Polymyxin B | 2,500IU | 5,000IU |
| Rifampicin | 5.0mg | 10.0mg |
| Trimethoprim | 5.0mg | 10.0mg |
| Cycloheximide | 50.0mg | 100.0mg |
Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.
Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Prl100 Piperacillin
RM80.00Brand:
Thermo ScientificTM OxoidTM
Prl30 Piperacillin
RM109.00Brand:
Thermo ScientificTM OxoidTM
Prl75 Piperacillin
RM0.00Brand:
Thermo ScientificTM OxoidTM
Pro Disk w/Reagent 25TST/KT
RM0.00Brand:
Thermo Scientific™ Remel™
Thermo Scientific™ Remel PRO Disc w/Reagent is used for identification of yeast and anaerobic microorganisms primarily Candida albicans and Clostridium difficile.
proBLUE DNA Stain
RM0.00Brand:
Cleaver Scientific
Agarose electrophoresis of DNA requires a staining step in order to visualise the DNA fragments. This can be done safely using methylene blue solution. Methylene blue binds to negatively charged DNA making DNA visible as blue bands within agarose following electrophoresis. Methylene blue solution is provided as a 10x concentrate. To use, dilute 10x concentrate by mixing one part of methylene blue with nine parts of water (Eg. 100 ml of 10x concentrate added to 900 ml water).
Proteinase K, Molecular Biology Grade – 2 ml
RM0.00Brand:
New England Biolabs
Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates.
- Highly characterized for more consistent performance
- Optimal activity and stability for up to 24 months
- Active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0.
- No detectable endonuclease, exonuclease, DNase or RNase contaminating activities
ProteoExtract® Native Membrane Protein Extraction Kit
RM2,877.00Brand:
Merck
Description
A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications.
Note: 1 kit is sufficient for up to 20 sample extractions.
ProteoExtract® Subcellular Proteome Extraction Kit
RM3,190.00Brand:
Merck
Description
Fast and reproducible extraction of subcellular proteomes from mammalian cells
ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells. The S-PEK takes advantage of the different solubilities of certain subcellular compartments in the four selected reagents. In the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. Cells or the parts of the cells remain attached to the plate during sequential extraction of subcellular compartments, until the appropriate extraction reagent is used. Thus, the early destruction of the cellular structure by enzymatic or mechanical detachment of cells from the tissue culture plate and any mixing of different subcellular compartments is prevented. For suspension-grown cells, extraction starts with gentle sedimentation and washing of the cells. The stepwise extraction delivers four distinct protein fractions from one sample:
– Cytosolic fraction (F1)
– Membrane/organelle protein fraction (F2)
– Nucleic protein fraction (F3)
– Cytoskeletal fraction (F4)
Proteins are obtained in the native state making the S-PEK suitable for many downstream applications such as 1D and 2D gel electrophoresis, immunoblotting, enzyme activity assays, and protein microarrays.
Sample size: 3-5×106 or 25-50 mg tissue.
ProteoExtract® Transmembrane Protein Extraction Kit
RM3,010.00Brand:
Merck
Description
The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
Proteose Peptone 500g
RM594.00Brand:
Thermo ScientificTM OxoidTM
Oxoid Proteose Peptone is a speciality peptone containing proteoses as defined in the USP.
- Particularly suitable for use in toxin production and in culture media for bacteria with wide-ranging nutritional requirements
ProtoScript II First Strand cDNA Synthesis Kit
Brand:
New England Biolabs
The ProtoScript® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA.
- Recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability (active up to 48°C)
- Generates cDNA up to 10 kb
- Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free water
