Yeast and Mold Agar 500g
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Yeast and Mold Agar is used for the isolation and maintenance of yeast and molds.
Yeast and Mold Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 3.0 |
| Malt extract | 3.0 |
| Peptone | 5.0 |
| Dextrose | 10.0 |
| Agar | 20.0 |
| Final pH 6.2 ± 0.2 @ 25°C |
Yeast and Mold Agar, Oxoid Preparation:
Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.
Precautions
For in vitro diagnostic use.
Do not use beyond the stated expiry date or if the product is caked, discoloured or shows any sign of deterioration.
Yeast Autolysate Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Yeast Autolysate Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.
- Add to GC Agar Base, Part No. CM0367B
- Each vial supplements 500mL of medium
Yeast Autolysate Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | |
| Yeast autolysate fractions | 5.0 g |
| Glucose | 0.5 g |
| Sodium bicarbonate | 0.075 g |
Yeast Autolysate Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053. Mix gently and pour into sterile Petri dishes.
Yeast Extract (Powder) 500g
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Thermo ScientificTM OxoidTM
Oxoid Yeast Extract Powder is a dried yeast autolysate which provides a nutritious source of nitrogen and vitamins.
- Recommended for plate count of organisms in water
- Increases yield of organisms of many media or fermentation broths
Yeast Extract Agar 500g, Oxoid
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Thermo ScientificTM OxoidTM
Carry out plate counts of microorganisms in water with Thermo Scientific™ Oxoid™ Yeast Extract Agar (Dehydrated). Yeast Extract Agar is made to the formula described by Windle Taylor for the plate count of microorganisms in water and is tested in accordance with ISO 1133:2014. It is a rich, nutritious medium for the recovery of a wide range of bacteria, yeast, and molds.
Yeast Extract Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 3.0 |
| Peptone | 5.0 |
| Agar | 15.0 |
| pH 7.2 ± 0.2 @ 25°C |
Yeast Extract Agar, Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
YeastBuster® Protein Extraction Reagent (for yeast cell lysis)
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Merck
Description
YeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
Yersinia Selective Agar Base 500g
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Thermo ScientificTM OxoidTM
- Oxoid Yersinia Selective Agar Base (Schiemann’s CIN Agar) is used for the isolation and enumeration of Yersinia enterocolitica from clinical specimens and foods.
- Also available Yersinia Selective Supplement, Part No. SR0109E. Industrial Reference: FDA.
Yersinia Selective Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Special peptone | 20.0 |
| Yeast extract | 2.0 |
| Mannitol | 20.0 |
| Sodium pyruvate | 2.0 |
| Sodium chloride | 1.0 |
| Magnesium sulphate | 0.01 |
| Sodium desoxycholate | 0.5 |
| Neutral red | 0.03 |
| Crystal violet | 0.001 |
| Agar | 12.5 |
| pH 7.4 ± 0.2 @ 25°C |
Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.
Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.
Yersinia Selective Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Yersinia Selective Supplement is used for the isolation of Yersinia enterocolitica.
- Add to Yersinia Selective Agar Base, Part No. CM0653B
- Each vial supplements 500mL of medium
Yersinia Selective Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500 ml of medium) | per vial | per litre |
| Cefsulodin | 7.5mg | 15mg |
| Irgasan | 2.0mg | 4.0mg |
| Novobiocin | 1.25mg | 2.5mg |
Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.
Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.
Zinc oxide for analysis EMSURE® ACS,Reag. Ph Eur
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Merck
Description
CAS number: 1314-13-2
Chemical Formula: ZnO
Molar Mass: 81.39 g/mol
Synonyms: –
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for Zinc oxide EMSURE® (Merck).
Zinc Oxide, CP, 500g
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Chemiz
Description
Formula: ZnO
Zinc oxide has a variety of uses including rubber and ceramic glaze manufacture, UV absorber, paint corrosion inhibitor and as nutrient supplement.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Zox30 Ceftizoxime
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Thermo ScientificTM OxoidTM
Multi Sub Horizontal Gel System
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Cleaver Scientific
Description
The new multisub Mini One is an all in one horizontal electrophoresis unit. Featuring a built in power supply with voltage options of 35V, 50V and 100V, the MSMINIONE is a versatile system suitable for a wide range of applications. A built in timer for runs from 0-99 minutes means you can set up your parameters and leave the system to complete the run automatically without fear of loosing bands.
The system comes complete with 2 gel casters for wide and mini gels as well as reversible combs for high throughput or high sample volume.
Includes multiSUB™miniONE electrophoresis system with Built in power supply 2 x MSO-UVL, 4 x MSO-UVS, 1 x MSO-GCL, 1 x MSO-GCS, 2 x MSO-1-5/9DS, 2 x MSO-1-12/22DS.
