DNase I (RNase-free)
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New England Biolabs
- Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends
- Degrades DNA template in transcription reactions
- Removes contaminating genomic DNA from RNA samples
- Isolated from a recombinant source
dsRNA Ladder – 25 gel lanes
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New England Biolabs
The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point.
This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels.
Dye Kit
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Cleaver Scientific
This simple kit will enable you to teach the principles behind gel electrophoresis without the need to view the samples on a UV or blue light transilluminator. The kit contains five dye samples labelled A to E with enough reagents to run two sets of six gels. Vial A contains a dye marker consisting all 4 dyes. The other vials B to E contain a single dye. When the samples are loaded into a gel and run on an electrophoresis unit the students will be able to watch the different coloured dyes separate by the size of the molecules, so simulating how DNA separates by different molecular weight.
ElectroLigase – 50 rxns
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New England Biolabs
ElectroLigase® combines T4 DNA Ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG. This combination is specifically formulated to promote robust ligation of all types of DNA ends (e.g., blunt, sticky, TA). It is directly compatible with electrocompetent cells used for transformation by electroporation, without desalting or purification.
- Directly compatible with electrocompetent cells
- No thawing needed as it maintains liquid form at -20°C
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Exo-CIP™ Rapid PCR Cleanup Kit, NEB
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New England Biolabs
- Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification
- Reaction complete in 4 minutes
- Thermolabile formulation can be heat inactivated in 1 minute at 80°C
- PCR product can be used directly in downstream applications
- Compatible with commonly-used reaction buffers
FastDNA 50ml Spin Kit for Soil
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MP Biomedicals
Up to 5 g of soil are processed by the FastPrep®-24 or FastPrep®-96 instrument with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter columns and removes humic acids/polyphenols that can inhibit PCR. The resulting pure DNA is suitable for PCR analysis and other downstream applications. Research use only. Key applications include isolation of bacterial, fungi, plant and genomic DNA from soil and evnironmental samples.
FastDNA-96 Soil Microbe DNA Kit
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MP Biomedicals
The FastDNA-96™ Soil Microbe DNA Kit quickly and efficiently isolates humic-free, PCR-ready genomic DNA from soil samples in approximately 50 minutes. Gram(+/-) bacteria, fungi, plant and animal tissues, algae, spores and many other members of a soil population including clay, sandy, silty, peaty, chalky, and loamy soils, are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Research use only. Key applications include isolation of PCR-Ready Genomic DNA from Soil Samples.
FastDNA™ Spin Kit
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MP Biomedicals
The FastDNA™ SPIN Kit, an updated version of the well-known FastDNA™ Kit, is used with any FastPrep® Instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 30 minutes. Features: Isolation of genomic DNA from plants, animals, bacteria, yeast, algae, and fungi using a silica spin fi lter method: Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument, Lyse and isolate DNA in less than 30 minutes from a variety of sample types, Flexible format eliminates need for separate DNA isolation kits, No hazardous organic reagents required, SPIN Filters are included to streamLine silica handling. Research Use Only. Key applications include DNA extraction and purification from virtually and sample.
FastDNA™ Spin Kit for Soil
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MP Biomedicals
The FastDNA® SPIN Kit is designed for use with any FastPrep® Instrument, plant and animal tissues, bacteria, algae, fungi spores and other members of a soil population are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by simultaneous impaction with lysing matrix particles. Features: Isolation of DNA from cells present in soil or other environmental samples, Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument, Easily isolate DNA from a variety of organisms in many different types of soil, Lyse and isolate DNA in less than 60 minutes, No hazardous organic reagents required. Research Use Only. Key applications include isolation of bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
FlashGel™ DNA System
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Lonza
Description
5 minute DNA analysis
The FlashGel™ System is the fastest way to separate DNA and the only way to watch DNA migration as it happens. This revolutionary new tool separates DNA in 2 – 7 minutes. Monitor gel runs right at the bench, without UV light. The highly sensitive system allows a 5-20X reduction in DNA levels – saving both time and money.
Please contact us for pricing and availability.
Gel Loading Dye, Purple (6X), no SDS – 4.0 ml, NEB
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New England Biolabs
Gel Loading Dye, Purple (6X), NEB is the premier purple loading dye for sharp, tight bands.
- No UV shadow
- Contains Ficoll® for brighter, tighter bands
- Contains EDTA to stop enzymatic reactions
- Compatible with agarose and non-denaturing polyacrylamide gels
- No UV shadow allows for publication grade images
- Brighter, sharper bands vs. glycerol-based loading dyes
- Stops enzyme reactions
GelStarTM Nucleic Acid Gel Stain, 10,000X (2 x 250 uL)
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Lonza
GelStarTM Nucleic Acid Gel Stain is a highly sensitive fluorescent stain for detecting both DNA and RNA. You can choose to either add GelStarTM Stain to your agarose solution prior to casting or to post stain your gels. GelStarTM Nucleic Acid Gel Stain exhibits exceptional signal-to-noise ratio with minimal background. Maximum sensitivity, detect as little as 20 pg of dsDNA or 3 ng of RNA.
Benefits
Applications
Hi-T4™ DNA Ligase
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New England Biolabs
Characteristics of Hi-T4 DNA Ligase:
- Hi-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved thermostability compared to T4 DNA Ligase
- Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids
- Supplied with both T4 DNA Ligase Buffer and StickTogether™ DNA Ligase Buffer
- Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Instant Sticky-end Ligase Master Mix and Blunt/TA Ligase Master Mix
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart
Hot Start Taq 2X Master Mix
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New England Biolabs
The industry standard with the hot start peformance
- Ideal for routine PCR applications
- Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
- 2X master mix format for easy reaction setup – just add template DNA and primers
Hot Start Taq DNA Polymerase
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New England Biolabs
The industry standard with hot start performance
- Ideal for routine PCR applications and molecular diagnostics
- High specificity and sensitivity
- Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
Immobilized T4 DNA Ligase
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New England Biolabs
Characteristics of Immobilized T4 DNA Ligase:
- Immobilized T4 DNA Ligase (IM T4 DNA Ligase) consists of T4 DNA Ligase covalently bound to magnetic beads.
- Easily remove enzyme from reaction using a magnet
- Avoid heat inactivation step, which may interfere with downstream applications
- Re-use immobilized enzymes
- Enable new workflows and applications
Instant Sticky-end Ligase Master Mix
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New England Biolabs
Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.
- No incubation time necessary to achieve ligation
- Efficiencies sufficient for successful sub-cloning of sticky-end substrates
- No thawing of the master mix is required as liquid state is maintained when stored at -20°C
- Simplified reaction setup with optimized ratio of enzyme and buffer components
- Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
LongAmp Taq 2X Master Mix
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New England Biolabs
Exceptional performance with long amplicons
- Optimized enzyme blend for amplification up to 30 kb
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification of complex or simple templates
LongAmp® Taq PCR Kit
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New England Biolabs
Exceptional performance with long amplicons
- Optimized enzyme blend for amplification up to 30 kb from low complexity templates and difficult templates, such has human genomic DNA
- 2X higher fidelity than Taq
- Kit contains 5X Reaction Buffer for optimal performance
Low Molecular Weight DNA Ladder
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New England Biolabs
This product is available in our Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
- Size range: 25 bp to 766 bp
- Value pricing
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
Low Range ssRNA Ladder
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New England Biolabs
The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing polyacrylamide-urea gels, and on denaturing or native agarose gels.
- Size range: 50 bases – 1,000 bases
- Compatible with denaturing polyacrylamide-urea gels, denaturing agarose gels and native agarose gels
- Convenient reference band at 300 bases
Luna Cell Ready One-Step RT-qPCR Kit
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New England Biolabs
Go direct from cells to RNA quantitation without purification
- Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)
- Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
- Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
- Features Luna WarmStart® RT paired with Hot Start Taq for increased thermostability and room temperature setup
- Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)
Luna Universal One-Step RT-qPCR Kit
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New England Biolabs
Make a simpler choice
- One product per application simplifies selection
- Convenient master mix formats and user-friendly protocols simplify reaction setup
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Experience best-in-class performance
Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase
Luna Universal Probe One-Step RT-qPCR Kit
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New England Biolabs
Make a simpler choice
- One product per application simplifies selection
- Convenient master mix formats and user-friendly protocols simplify reaction setup
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Experience best-in-class performance
Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase
