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This product has been discontinued, please refer to SR0258E

Thermo Scientific™ Oxoid OCLA (ISO) Differential Supplement is a differential supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Differential Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Thermo Scientific™ Oxoid OCLA (ISO) Selective Supplement is a selective supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Selective Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued. Please contact us for more information.

Oxoid ORSAB Selective Supplement (Oxacillin Resistance Screening Agar Base Selective Supplement) is added to Oxacillin Resistance Screening Agar Base, Part No. CM1008B.

• Add to Oxacillin Resistance Screening Agar Base, Part No. CM1008B
• Each vial supplements 500mL of medium

ORSAB Selective Supplement, Oxoid Composition

ORSAB Selective Medium Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
ORSAB Selective Supplement should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

• Oxoid Oxacillin Resistance Screening Agar Base (ORSAB) is a medium for screening clinical samples for the presence of oxacillin-resistant Staphylococci.
• Also available ORSAB Selective Supplement, Part No. SR0195E.

Oxacillin Resistance Screening Agar Base (ORSAB), Oxoid Composition

Oxacillin Resistance Screening Agar, Oxoid Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Peptone water (CM0009B), Oxoid, is a basal medium that used as a growth medium or as the basis of carbohydrate fermentation media. A pure culture in Peptone Water is a convenient inoculum for a series of fermentation tubes or other diagnostic media.

Directions

Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Isolate and enumerate yeasts and molds from dairy and food products and prepare Aspergillus niger for the Harmonised Microbial Limit Tests from international pharmacopoeia with Thermo Scientific™ Oxoid™ Potato Dextrose Agar (Dehydrated). This medium is suitable for the detection and enumeration of heat resistant molds in thermally processed fruits and fruit products.

Pigment production is a critical part of the identification of the fungus. The agar used in the medium is carefully screened to ensure correct pigment production by fungi such as Fusaria spp.

Composition of Potato Dextrose Agar

Preparation of Potato Dextrose Agar, Oxoid
Suspend 39g in 1 litre of purified water and heat up to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Ensure the solution is well mixed before pouring.

To suppress the growth of bacteria, you can acidify the medium to pH 3.5 by adding 1 ml of lactic acid 10% SR0021 to each sterilized medium at 50°C. Note that the medium must not be heated after the addition of acid as it will destroy the gelling properties and results in hydrolysis of agar.

Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product is temporarily unavailable. Please contact us for more information.

Oxoid Saline Tablets is used for preparation of 500mL of 0.85% isotonic saline. For Laboratory Use Only.

This product has been discontinued. The alternative to this product is Sensitest Agar, 500g.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

A selective supplement for the detection, enumeration and presumptive identification of Listeria
monocytogenes and other Listeria species from food, animal feed and environmental samples
according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference
methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Directions:
Aseptically add 1 vial warmed to 48°C to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Selective Supplement (SR0257E) reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life

Store the selective (SR0257)  supplements at 2-8°C away from light. Ensure all product is used before the expiry date.

Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.

Brilliance Listeria Agar (ISO), Oxoid Composition

Brilliance Listeria Agar (ISO), Oxoid Preparation:

Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance™ Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance™ Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.

Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label

Brand:

Thermo Scientific^TM Oxoid^TM

A differential supplement for the detection, enumeration, and presumptive identification of Listeria monocytogenes and other Listeria species from food, animal feed and environmental samples according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Differential Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Differential Supplement, Oxoid Directions:
Aseptically add 5ml of sterile distilled water to 1 vial and mix gently to dissolve. Aseptically add the vial contents to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Differential Supplement (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the differential supplements (SR0258) at 2-8°C away from light. Do not freeze the differential supplement solution. Ensure all product is used before the expiry date.

A stabilised emulsion of egg yolk for use in culture media. It may be added directly to nutrient media for the identification of Clostridium, Bacillus and Staphylococcus species by their lipase activity.

Egg Yolk Emulsion, Oxoid Directions:
Suspend 21.5g of MYP Agar (CM0929) in 450ml of distilled water and bring gently to the boil to
dissolve. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and
aseptically add 50ml of Egg Yolk Emulsion (SR0047C) and the contents of one vial of Polymyxin B
Supplement (SR0099E) reconstituted as directed. Mix well and pour into sterile Petri dishes.
Alternatively, suspend 8g of Blood Agar Base (CM0055) in 200ml of distilled water and add 2g of
sodium chloride. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and
aseptically add 20ml Egg Yolk Emulsion (SR0047C). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Keep at temperatures between 2° and 8 °C. Use before expiry date on the label.

Precautions
On this medium Bacillus cereus is indistinguishable from Bacillus thuringiensis.
Identify Bacillus cereus by colony form, colour, egg yolk hydrolysis and confirm with cell and spore morphology.
Occasional strains of Bacillus cereus show weak or negative egg yolk reactions.

This product has been discontinued. Please contact us for more information.

Oxoid Tinsdale Supplement is used for the cultivation of Corynebacterium diphtheriae.

• A supplement for Tinsdale Medium, Part No. CM0487B
• Each vial supplements 200mL of medium

Tinsdale Supplement, Oxoid Composition

Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.

Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).

This product has been discontinued. Please contact us for more information.

Oxoid VCN Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

VCN Selective Supplement, Oxoid Composition

VCN Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Thayer Martin Medium, cooled to 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 plus the rehydrated contents of one vial of Vitox SR0090. Mix gently and pour into sterile Petri dishes.

This item is not available at the moment. Please refer to the alternative, Violet Red Bile Glucose Agar (CM0485B). However, please note that this product does not conform to ISO.

Selectively enumerate Enterobacteriaceae in food, animal feed, and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated) while adhering to ISO 21528 and ISO 11133:2014. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBGA (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBGA (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBGA (ISO) plates can be stored for up to 2 weeks when made from CM1082 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBGA (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBGA (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

This product has been discontinued. Please contact us for more information.

• Oxoid VRE Broth Base (Vancomycin Resistant Enterococci Broth Base) is for selective isolation of vancomycin-resistant enterococci and high Level aminoglycoside-resistant enterococci.
• Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Broth Base, Oxoid Composition

VRE Broth, Oxoid Preparation:
Suspend 37.0g of VRE Broth Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then mix well and distribute into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.