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Listeria Selective Enrich Supplement 10×2.25 Litres

Brand:

Thermo ScientificTM OxoidTM

Oxoid Listeria Selective Enrichment Supplement, Modified is used for the enrichment of Listeria spp.

  • Modified with 10mg/L acriflavin
  • Add to Listeria Enrichment Broth, Part No. CM0862B
  • Each vial supplements 2.25L of medium

Listeria Selective Enrichment Supplement (Modified), Oxoid Composition

Vial contents (each vial is sufficient for 2,250mls of medium)
per vial
per litre
Nalidixic acid

90.0mg

40.0mg
Cycloheximide

112.5mg

50.0mg
Acriflavine hydrochloride

22.5mg

10.0mg

 

Listeria Selective Enrichment Medium Preparation:
Aseptically add 10ml of distilled water to one vial and invert gently to dissolve. Add the vial contents to 2.25 litres of Listeria Enrichment Broth Base. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required .

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Listeria Selective Supplement (Oxford) 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Listeria Selective Supplement (Oxford) is used for the detection of Listeria monocytogenes.
  • Add to Listeria Selective Agar Base, Part No. CM0856B.

Listeria Selective Supplement (Oxford), Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)

per vial

per litre
Cycloheximide
200mg
400mg

Colistin sulphate

10.0mg

20.0mg

Acriflavine

2.5mg

5.0mg

Cefotetan

1.0mg

2.0mg

Fosfomycin

5.0mg

10.0mg

 

Listeria Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Listeria Selective Agar Base (Oxford Formulation) CM0856, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Listeria Selective Supplement 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

Oxoid Listeria Selective Enrichment Supplement is used for the enrichment of Listeria spp.

  • Add to Listeria Enrichment Broth, Part No. CM0862B
  • Each vial supplements 500mL of medium

Listeria Selective Enrichment Supplement, Oxoid Composition

Vial contents: (each vial is sufficient for 500ml of medium)
per vial
per litre
Nalidixic acid

20.0mg

40.0mg
Cycloheximide

25.0mg

50.0mg
Acriflavine hydrochloride

7.5mg

15.0mg

 

Listeria Selective Enrichment Medium Preparation:
Reconstitute one vial as directed, and add the contents to 500ml of  Listeria Enrichment Broth Base (CM0862). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before distributing into sterile containers in volumes as required.

Storage conditions and Shelf life
Listeria Selective Enrichment Supplement SR0141 as supplied should be stored at 2-8°C. When stored as directed the reagents are stable until the expiry date printed on the label.

Precautions
Listeria Selective Enrichment Supplement contains cycloheximide and is toxic if swallowed, inhaled or by skin contact. As a precaution when handling wear gloves and eye/face protection.
Acriflavine is activated by light and may form compounds inhibitory for Listeria.

Litmus Milk, for Microbiology, NutriSelect™ Plus, Sigma

Brand:

Sigma-Aldrich

Application

For maintenance of Lactobacilli and for determining the action of bacteria on milk.

Typical Formula *
gm/litre
Litmus

0.5

Skim Milk Powder

100

Sodium Sulfite

0.5

Final pH: 6.8±0.2 (25 °C)

 

Liver Broth 500g

Brand:

Thermo ScientificTM OxoidTM

Cultivate and enumerate saccharolytic or putrefactive mesophilic and thermophilic anaerobes from the food samples with Thermo Scientific™ Oxoid™ Liver Broth Culture Medium (Dehydrated). The medium contains liver particles which support luxurious growth for anaerobes.

Liver Desic Bacteriological

Brand:

Thermo ScientificTM OxoidTM

Prepare Liver infusion media with Thermo Scientific™ Oxoid™ Liver Desiccated Bacteriological. Liver Desiccated is prepared by the dehydration of fresh pork livers under carefully controlled conditions designed to ensure maximum retention of nutritive properties, and is equivalent to five times its weight of fresh liver.

  • Dehydrated whole liver, specially manufactured for the preparation of infusion media.
  • Not all products are available for sale in all territories. Please inquire.
  • Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Liver Digest Neutralised 500g

Brand:

Thermo ScientificTM OxoidTM

The Oxoid Liver Digest Neutralized is a biologically standardized papaic digest of ox liver for use as a source of nutrients in microbiological culture media.

Lom10 Lomefloxacin

Brand:

Thermo ScientificTM OxoidTM

This product has been discontinued. Please contact us for more information.
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Lomefloxacin Antimicrobial Susceptibility Disks.

LongAmp Taq 2X Master Mix

Brand:

New England Biolabs

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb
  • 2X higher fidelity than Taq
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification of complex or simple templates

LongAmp® Taq PCR Kit

Brand:

New England Biolabs

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb from low complexity templates and difficult templates, such has human genomic DNA
  • 2X higher fidelity than Taq
  • Kit contains 5X Reaction Buffer for optimal performance

Low Molecular Weight DNA Ladder

Brand:

New England Biolabs

This product is available in our Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

  • Size range: 25 bp to 766 bp
  • Value pricing
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes

Low Range ssRNA Ladder

Brand:

New England Biolabs

The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing polyacrylamide-urea gels, and on denaturing or native agarose gels.

  • Size range: 50 bases – 1,000 bases
  • Compatible with denaturing polyacrylamide-urea gels, denaturing agarose gels and native agarose gels
  • Convenient reference band at 300 bases

Luna Cell Ready One-Step RT-qPCR Kit

Brand:

New England Biolabs

Go direct from cells to RNA quantitation without purification

  • Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)
  • Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
  • Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
  • Features Luna WarmStart® RT paired with Hot Start Taq for increased thermostability and room temperature setup
  • Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)

Luna Universal One-Step RT-qPCR Kit

Brand:

New England Biolabs

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness

Luna Universal Probe One-Step RT-qPCR Kit

Brand:

New England Biolabs

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness

Luna Universal Probe qPCR Master Mix

Brand:

New England Biolabs

Rapid, sensitive and precise probe-based qPCR detection and quantitation of target DNA and cDNA sequences.

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Luna Universal qPCR Master Mix

Brand:

New England Biolabs

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences.

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Luna® Cell Ready Lysis Module

Brand:

New England Biolabs

  • Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
  • Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
  • Minimal sample loss compared to alternative RNA extraction methods, especially at low cell input
  • Compatible with high-throughput applications: cell lysis can take place at room temperature
  • Optimal performance when paired with Luna Universal One-Step RT-qPCR kits: NEB #E3005 (dye-based) or NEB #E3006 (probe-based)
  • Complete lysis and one-step RT-qPCR workflow available: NEB #E3030 (dye-based) or NEB #E3031 (probe-based)

Luna® Cell Ready Probe One-Step RT-qPCR Kit

Brand:

New England Biolabs

Go direct from cells to RNA quantitation without purification

  • Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (probe)
  • Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
  • Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
  • Features Luna WarmStart® RT paired with Hot Start Taq for increased thermostability and room temperature setup
  • Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)

LunaScript®RT SuperMix Kit

Brand:

New England Biolabs

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

Make a simpler choice

  • Single-tube SuperMix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna® Reverse Transcriptase
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
  • Combine with Luna qPCR master mixes for robust RT-qPCR results

Experience best-in-class performance

  • Less than 15 minute first-strand cDNA synthesis protocol
  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Illuminate your two-step RT-qPCR

  • Novel, thermostable Luna Reverse Transcriptase improves performance
  • Consistent linearity, sensitivity, and capacity for reliable RNA quantification

LYSINE DECARBOXYLASE 5 x 20 tablets

Brand:

Thermo ScientificTM OxoidTM

Thermo Scientific™ Oxoid Lysine Decarboxylase Broth Tablets detect lysine decarboxylase production by Salmonella and some other Enterobacteriaceae.

Lysine Iron Agar 500g

Brand:

Thermo ScientificTM OxoidTM

Detect salmonellae including Salmonella arizonae with the diagnostic Thermo Scientific™ Oxoid™ Lysine Iron Agar (Dehydrated). This differential medium detects salmonellae (including lactose fermenting Salmonella arizonae) by lysine decarboxylase activity and H2S production.

Lysine Iron Agar, Oxoid Composition

Typical Formula*

gm/litre

Bacteriological peptone

5.0

Yeast extract

3.0

Glucose

1.0

L-lysine

10.0

Ferric ammonium citrate

0.5

Sodium thiosulphate

0.04

Bromocresol purple

0.02

Agar

14.5

pH 6.7 ± 0.2 @ 25°C

 

Lysine Iron Agar, Oxoid Preparation:
Suspend 34g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into tubes and sterilise by autoclaving at 121° C for 15 minutes. Cool the tubes in an inclined position to form slants with deep butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C.

Lysine Medium 500g

Brand:

Thermo ScientificTM OxoidTM

Isolate and enumerate wild yeasts encountered in brewing while suppressing pitching yeasts with Thermo Scientific™ Oxoid™ Lysine Medium (Dehydrated). This complex, synthetic medium is based on the original formula of Morris & Eddy which utilizes the susceptibility of pitching yeasts to lysine to differentiate it from the wild yeast.

Lysine Medium, Oxoid Composition

Typical Formula*

gm/litre

Glucose

44.5

Potassium dihydrogen phosphate

1.78

Magnesium sulphate

0.89

Calcium chloride fused

0.178

Sodium chloride

0.089

Adenine

0.00178

DL-methionine

0.000891

L-histidine

0.000891

DL-tryptophane

0.000891

Boric acid

0.0000089

Zinc sulphate

0.0000356

Ammonium molybdate

0.0000178

Manganese sulphate

0.0000356

Ferrous sulphate

0.0002225

Lysine

1.0

Inositol

0.02

Calcium pantothenate

0.002

Aneurine

0.0004

Pyridoxine

0.0004

p-aminobenzoic acid

0.0002

Nicotinic acid

0.0004

Riboflavin

0.0002

Biotin

0.000002

Folic acid

0.000001

Agar

17.8

pH (see directions)

 

Lysine Medium, Oxoid Preparation:
Suspend 6.6g in 100ml distilled water containing 1.0ml Potassium lactate SR0037. Bring to the boil to dissolve completely. Agitate frequently to prevent superheating. Cool to 50°C and add 0.1ml of lactic acid 10% SR0021 to adjust to pH 4.8 ± 0.2. Dispense into Petri dishes and remove surface moisture by drying at 37°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Lzd10 Linezolid

Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Linezolid Antimicrobial susceptibility Disks.