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Isolate Legionella from environmental samples and clinical specimens using Thermo Scientific™ Oxoid™ Legionella CYE Agar Base when used with Legionella Growth Supplement SR01110. Legionella CYE Agar Base is a non-selective medium that exhibits optimal recovery of legionellaceae in short incubation periods from both environmental samples and clinical specimens.

Legionella CYE Agar Base, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

• Oxoid Listeria Enrichment Broth is used for the enrichment of Listeria species.
• Also available Listeria Selective Supplement, Part No. SR0140E or SR0141E and Modified Listeria Selective Enrichment Supplement, Part No. SR0206E or SR0206B.

Listeria Enrichment Broth Base, Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 18g in 500ml of distilled water. Add the contents of one vial of Listeria Selective Enrichment Supplement (SR0141), reconstituted with 2ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140. Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against Listeria.
Supplement SR0141 used in this medium contains a toxic concentration of cycloheximide. Note the precautions to be taken under HAZARDS.

• Oxoid Listeria Enrichment Broth Base (UVM Formulation) is a two-step selective enrichment (USDA-FSIS) method.
• Also available Listeria Primary Enrichment Supplement-UVM I, Part No. SR0142E or SR0142B and Listeria Secondary Selective Enrichment Supplement-UVM II, Part No. SR0143E.
  Listeria Enrichment Broth Base (UVM formulation) is to be used with the appropriate supplement for UVMI broth or UVMII broth.

Listeria Enrichment Broth Base (UVM formulation), Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.

To Prepare Listeria Primary Selective Enrichment Medium (UVM I):
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

To Prepare Listeria Secondary Selective Enrichment Medium (UVM II):
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

Thermo Scientific™ Oxoid Listeria Primary Enrichment Supplement (UVMI) is a primary selective enrichment supplement for the isolation of listeria when used with Listeria Enrichment Broth Base (UVM Formulation) CM0863.

Oxoid Listeria Primary Enrichment Supplement (UVMI), Oxoid Composition

Listeria Primary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

Oxoid Listeria Secondary Selective Enrichment Supplement, UVMII is used in a two-step selective enrichment (USDS-FSIS) method for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth Base (UVM formulation), Part No. CM0863B
• Each vial supplements 500mL of medium

Listeria Secondary Selective Enrichment Supplement (UVMII), Oxoid Composition

Listeria Secondary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

• Oxoid Listeria Selective Oxford Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available Listeria Selective Supplement, Part No. SR0140E and Modified Listeria Selective Supplement, Part No. SR0206E or SR0206B.

Listeria Selective Oxford Agar Base, Oxoid Composition

Listeria Selective Oxford Agar, Oxoid Preparation:
Suspend 27.75 of the Listeria Selective Agar Base (Oxford Formulation) in 500ml of distilled water. Bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Listeria Selective Supplement (Oxford Formulation) or Modified Listeria Selective Supplement (Oxford) SR0206, SR0140 reconstituted with 5ml of 70% ethanol. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Oxoid Listeria Selective Enrichment Supplement, Modified is used for the enrichment of Listeria spp.

• Modified with 10mg/L acriflavin
• Add to Listeria Enrichment Broth, Part No. CM0862B
• Each vial supplements 2.25L of medium

Listeria Selective Enrichment Supplement (Modified), Oxoid Composition

Listeria Selective Enrichment Medium Preparation:
Aseptically add 10ml of distilled water to one vial and invert gently to dissolve. Add the vial contents to 2.25 litres of Listeria Enrichment Broth Base. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required .

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Listeria Selective Supplement (Oxford) is used for the detection of Listeria monocytogenes.
• Add to Listeria Selective Agar Base, Part No. CM0856B.

Listeria Selective Supplement (Oxford), Oxoid Composition

Listeria Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Listeria Selective Agar Base (Oxford Formulation) CM0856, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Oxoid Listeria Selective Enrichment Supplement is used for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth, Part No. CM0862B
• Each vial supplements 500mL of medium

Listeria Selective Enrichment Supplement, Oxoid Composition

Listeria Selective Enrichment Medium Preparation:
Reconstitute one vial as directed, and add the contents to 500ml of  Listeria Enrichment Broth Base (CM0862). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before distributing into sterile containers in volumes as required.

Storage conditions and Shelf life
Listeria Selective Enrichment Supplement SR0141 as supplied should be stored at 2-8°C. When stored as directed the reagents are stable until the expiry date printed on the label.

Precautions
Listeria Selective Enrichment Supplement contains cycloheximide and is toxic if swallowed, inhaled or by skin contact. As a precaution when handling wear gloves and eye/face protection.
Acriflavine is activated by light and may form compounds inhibitory for Listeria.