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Thermo Scientific™ Oxoid Soy Peptone is obtained by the hydrolysis of soya flour and complies with the USP specification.

Thermo Scientific™ Remel™ Sputasol is a liquifying agent for sputum samples.

• Each vial dilutes in sterile distilled water to make 100mL

Sputasol, Oxoid Composition

Sputasol, Oxoid Preparation:
Aseptically add the contents of one vial (7.5ml) to 92.5ml of sterile distilled water. Use the working solution immediately or store at 2-8°C for upto 48 hours only.

• Oxoid STAA Agar Base is used for the isolation of Brochothrix thermosphactafrom food samples.
• Also available STAA Selective Supplement, Part No. SR0151E and STA Selective Supplement, Part No. SR0162E.

STAA Agar Base, Oxoid Composition

STAA Agar, Oxoid Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add:

a) for STAA Medium the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

b) for STA Medium the contents of 1 vial of STA Selective Supplement SR0162 reconstituted as directed.
Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid STAA Selective Supplement is used for the isolation of Brochothrix thermosphacta from food samples.

• Add to STAA Agar Base, Part No. CM0881B
• Each vial supplements 500mL of medium

STAA Selective Supplement, Oxoid Composition

STAA Selective Medium Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in contact with skin.

Thermo Scientific™ Oxoid Standard Plate Count Agar (Plate Count Agar) is a standard medium corresponding to the APHA formulation used for milk, food and dairy products.

Standard Plate Count Agar (Plate Count Agar), Oxoid Composition

Standard Plate Count Agar (Plate Count Agar), Oxoid Preparation:
Suspend 23.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into bottles and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Staphylococcus Medium No.110 is a selective medium is used for isolation and differentiation of pathogenic Staphylococci.

Staphylococcus Medium No.110, Oxoid Composition

Staphylococcus Medium No.110, Oxoid Preparation:
Suspend 150g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Disperse the precipitate by gentle agitation before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Enterococcus faecalis may grow on this medium as tiny colonies with slight mannitol fermentation.
The high salt content in Staphylococcus Medium No.110 may interfere with the coagulase reaction. Always sub-culture to a non-inhibitory medium before testing.

Oxoid Streptococcus Supplement is used for the isolation of Streptococci.

• Add to Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Streptococcus Selective Supplement, Oxoid Composition

Streptococcus Selective Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500ml of sterile Columbia Blood Agar Base containing 5% Defibrinated Horse Blood SR0050 cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Stuart Transport Medium is a semi-solid non-nutritional transport medium for fastidious, pathogenic organisms.

Stuart Transport Medium, Oxoid Composition

Stuart Transport Medium, Oxoid Preparation:
Suspend 16g in 1 litre of distilled water. Bring to the boil to dissolve completely and dispense into screw-capped 7ml bottles. Fill each bottle to the brim, tighten the cap and sterilise by autoclaving at 121°C for 15 minutes. When sufficiently cool to handle, mix by inversion.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 15-25°C.

Precautions
A small amount of blue colour at the top of the bottle indicates oxidation. If this colour extends down into the medium it should be discarded.
Avoid prolonged heating in open flasks, during the preparation of the medium, because thioglycollate is volatile.
Sodium glycerophosphate may be metabolised by some organisms and thus promote their growth.

Selectively isolate pathogenic Vibrio species with Thermo Scientific™ Oxoid™ TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated). The medium has shown to be the most satisfactory and reproducible formula regardless of the complex composition. Unlike Lauryl Sulphate Tellurite Agar it does not require further additives or aseptic additions of blood. It also possesses superior growth characteristics for Vibrio species, compared with tellurite media. While inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios after overnight incubation at 35°C.

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Composition

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE.
Pour plates without further heating and dry before use.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The identification of the various Vibrio species on TCBS Medium is presumptive and further tests are required for confirmation.
Yellow colonies on TCBS Medium will give unsatisfactory oxidase reactions.
Colonies taken from TCBS Medium are `sticky’ and react poorly in slide agglutination tests. Subculture to nutrient agar is required before slide agglutination tests can be carried out.
Some strains of Vibrio vulnificus produce better recovery at 30°C.

• Oxoid TTC Solution (0.125%) is used for the enumeration and identification of coliform bacteria.
• Add to Tergitol 7 Agar, Part No. CM0793B.
• Each vial for 100mL medium.