Supreme TC Grade Agar, Plant Cell Technology
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Plant Cell Technology
A polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae. Typical usage rate of 6 – 12 g/L medium. Supreme agar offers greater clarity of plant culture media.
Gel Strength: 1050 GM/CM2
Application:
Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.
Sxt25 Sulfamethoxazole/Trimethoprim
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Thermo ScientificTM OxoidTM
SYBR® Green II RNA gel stain, 10,000 × in DMSO (Sigma-Aldrich)
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Sigma
Description
CAS Number : 172827-25-7
Concentration : 10,000 × in DMSO
Usage : mL sufficient for 100 mini-gels
Synonyms : RNA gel dye, SYBR® RNA dye, safer gel stain
SYBR® Green II is a highly sensitive stain for post electrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels. SYBR® Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA than to double stranded DNA.
Application :
-for the quantification of ribonucleic acid (RNA) in Escherichia coli[1]
-for staining 5′-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis[2]
-to stain formaldehyde agarose gels to visualize RNA bands[3]
T.C.B.S Cholera Medium 500g
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Thermo ScientificTM OxoidTM
Selectively isolate pathogenic Vibrio species with Thermo Scientific™ Oxoid™ TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated). The medium has shown to be the most satisfactory and reproducible formula regardless of the complex composition. Unlike Lauryl Sulphate Tellurite Agar it does not require further additives or aseptic additions of blood. It also possesses superior growth characteristics for Vibrio species, compared with tellurite media. While inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios after overnight incubation at 35°C.
TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 5.0 |
| Bacteriological peptone | 10.0 |
| Sodium thiosulphate | 10.0 |
| Sodium citrate | 10.0 |
| Ox Bile | 8.0 |
| Sucrose | 20.0 |
| Sodium chloride | 10.0 |
| Ferric citrate | 1.0 |
| Bromothymol blue | 0.04 |
| Thymol blue | 0.04 |
| Agar | 14.0 |
| pH 8.6 ± 0.2 @ 25°C |
TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE.
Pour plates without further heating and dry before use.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
The identification of the various Vibrio species on TCBS Medium is presumptive and further tests are required for confirmation.
Yellow colonies on TCBS Medium will give unsatisfactory oxidase reactions.
Colonies taken from TCBS Medium are `sticky’ and react poorly in slide agglutination tests. Subculture to nutrient agar is required before slide agglutination tests can be carried out.
Some strains of Vibrio vulnificus produce better recovery at 30°C.
T.T.C. (Red Spot) Flexi-Slide
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Thermo ScientificTM OxoidTM
T.T.C. And Malt Extract Flexi-Slide
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Thermo ScientificTM OxoidTM
T.T.C. Solution 1 X 10 Vials
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Thermo ScientificTM OxoidTM
- Oxoid TTC Solution (0.125%) is used for the enumeration and identification of coliform bacteria.
- Add to Tergitol 7 Agar, Part No. CM0793B.
- Each vial for 100mL medium.
T.T.C.Solution 1% (10x5ml)
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid T.T.C. Solution is a solution for the addition to KF Streptococcus Agar for the enumeration of entercocci.
T.T.C. Solution, Oxoid Composition
| Vial contents (each vial is sufficient for 500 ml of medium) | |
| 2,3,5-Triphenyltetrazolium chloride (1%) | 5ml |
Medium Preparation:
Aseptically add the contents to 500ml of KF Streptococcus Agar CM0701 cooled to approximatly 50°C. Mix gently and pour into sterile Petri dishes.
T4 DNA Ligase, NEB
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New England Biolabs
Characteristics of T4 DNA Ligase, NEB:
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
- This enzyme will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
- T4 DNA ligase will seal nicks for these DNA substrates.
- The industry standard for performance and quality
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
TAE Buffer Solution
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
TAE buffer 50x solution: TAE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This 25x and 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.
Tannic acid, ACS reagent (Sigma-Aldrich)
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Sigma-Aldrich
Description:
CAS number: 1401-55-4
Chemical Formula: C76H52O46
Molar Mass: 1701.20 g/mol
Synonyms: Gallotannin, Tannin
General description
Tannic acid (TA) is a natural, non-toxic organic acid with corrosion inhibitive property for metals and alloys. A chemiluminescence (CL) system of KIO4 (potassium periodate)–H2O2 (hydrogen peroxide)–Tween40 has been employed in the detection of tannic acid in industrial wastewater. Tannic acid hydrolyzes to form mainly glucose and gallic acid. Its astringent property is due to the presence of polyphenolic groups.
Application
Tannic acid may be used in the following processes:
• Synthesis of ferric tannate complexes by reacting with metallic iron.
• As a protein deproteinizing agent in genomic and plasmid DNAs preparation.
• As a reductant in the synthesis of metal nanoparticles.
Taq 2X Master Mix – 500 rxns
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New England Biolabs
Taq DNA Polymerase is the industry standard
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- 2X master mix format for easy reaction setup – just add template DNA and primers
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
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New England Biolabs
The industry standard
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- Mg-free buffer offers flexibility to adjust magnesium concentrations for use with microarray or DHPLC applications
Taq DNA Polymerase with Standard Taq Buffer, NEB
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New England Biolabs
Taq DNA Polymerase, NEB is a thermostable DNA polymerase that possesses 5′ to 3′ polymerase activity, that makes it an excellent industry standard for PCR.
Key features of Taq DNA Polymerase, NEB:
- Isolated from a recombinant source
- Robust and reliable reaction
- Incorporates dUTP, dITP and fluorescently -labeled nucleotides
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- Hot start version available
Taq DNA Polymerase, NEB is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
Taq DNA Polymerase with ThermoPol Buffer
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New England Biolabs
Taq DNA Polymerase is the industry standard for PCR
- Ideal for routine PCR applications
- ThermoPol Buffer delivers high product yield under demanding conditions
- Exceptional value in terms of cost per unit
Taq PCR Kit
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New England Biolabs
The industry standard
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- Kit format includes optimized reagents and enables flexible experimental design for 200 PCR reactions
TB Decolorizer (3% acid alc) BTLx5/250ml
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Thermo Scientific™ Remel™
Thermo Scientific™ Remel TB Decolorizer is for TB Kinyoun and Ziehl-Neelsen stains.
TB Kinyoun Carbolfuchsin BTL/250ml
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Thermo Scientific™ Remel™
Thermo Scientific™ Remel TB Kinyoun Carbolfuchsin is a primary stain for AFB and Cryptosporidium modified acid-fast staining procedures.
TB Methylene Blue BTL/250ml
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Thermo Scientific™ Remel™
Thermo Scientific™ Remel TB Methylene Blue is a counterstain for AFB and Cryptosporidium modified acid-fast stain using TB Kinyoun or TB Ziehl-Neelsen stains.
TBX Medium 500g
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Thermo ScientificTM OxoidTM
Detect and enumerate Escherichia coli in food and animal feed samples with Thermo Scientific™ Oxoid™ Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated). The medium meets the formulation and performance criterion laid down in ISO 16649 and ISO 11133:2014.
TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.
Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 20.0 |
| Bile Salts No. 3 | 1.5 |
| Agar | 15.0 |
| X-glucuronide | 0.075 |
| pH 7.2 ± 0.2 @ 25°C |
Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Preparation:
Suspend 36.6g of TBX Medium in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour the medium into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
TE Buffer
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
‘TE is deriving from ‘Tris’, a common pH buffer and ‘EDTA’, a cation chelators like Mg2+. TE buffer is normally used in molecular biology to solubilize DNA, RNA or cDNA while protecting it from degradation, or used in the cell culture. Here, we provide TE buffer in 10x or 100x with pH ranging from 7.4 to 8.
TE Buffer Solution pH 8.0 (Merck)
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Merck
Description
1X TE Solution, pH 8.0 (10mM TRIS-HCl, 1mM EDTA). Molecular Biology grade.
Specifications:
Conductivity at 25°C: 775-975 µmho
DNase: None detected
pH of solution at 25°C: 7.90-8.10
Protease: None detected
RNase: None detected
Te10 Tetracycline
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Thermo ScientificTM OxoidTM
Te30 Tetracycline
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Thermo ScientificTM OxoidTM
