Tryptone Bile Agar 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
The Oxoid Tryptone Bile Agar is used for the detection and enumeration of E.coli biotype 1 in foods.
Tryptone Bile Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 20.0 |
| Bile salts No.3 | 1.5 |
| Agar | 15.0 |
| pH 7.2 ± 0.2 @ 25°C |
Tryptone Bile Agar, Oxoid Preparation:
Suspend 36.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour 12-15ml of the medium into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Tryptone Glucose Extract Agar 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Oxoid Tryptone Glucose Extract Agar is a standard method medium for examination of water and dairy products.
Tryptone Glucose Extract Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| `Lab-Lemco’ powder | 3.0 |
| Tryptone | 5.0 |
| Glucose | 1.0 |
| Agar | 15.0 |
| pH 7.0 ± 0.2 @ 25°C |
Tryptone Glucose Extract Agar, Oxoid Preparation:
Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. When the dilution of the original specimen is greater than 1 in 10, add 10 ml of sterile 10% solution of Skim Milk Powder LP0031 per litre.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Tryptone Soya Agar 2.5kg
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Grow a wide variety of microorganisms with Thermo Scientific ™ Oxoid ™ Tryptone Soya Agar EP/USP/JP/BP (Dehydrated). A general purpose agar medium containing two peptones, supports the growth of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. Tryptone Soya Agar is recommended as a reference medium when testing selective media to measure the degree of inhibition.
| Typical Formula | gm/litre |
| Pancreatic digest of casein | 15.0 |
| Enzymatic* digest of soya bean | 5.0 |
| Sodium chloride | 5.0 |
| Agar | 15.0 |
| pH 7.3 ± 0.2 @ 25°C |
*Contains papain
Preparation of Tryptone Soya Agar, Oxoid
First, you add 40g of tryptone soya agar to 1 litre of distilled water (purified as required). You can boil to dissolve the powder completely. After dissolved, you can sterilise the medium by autoclaving at 121°C for 15 minutes.
Storage Condition and Shelf Life of Tryptone Soya Agar, Oxoid
You can store the dehydrated tryptone soya agar at 10-30°C and use before the expiry date on the label or store the prepared plates of a medium at 2-8°C.
Tryptone Soya Agar 500g, Oxoid
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Tryptone Soya Agar, Oxoid (dehydrated) is suitable to grow a wide variety of microorganisms. It’s a general-purpose agar medium containing two peptones, which supports the growth of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. Tryptone Soya Agar is recommended as a reference medium when testing selective media to measure the degree of inhibition.
| Typical Formula | gm/litre |
| Pancreatic digest of casein | 15.0 |
| Enzymatic* digest of soya bean | 5.0 |
| Sodium chloride | 5.0 |
| Agar | 15.0 |
| pH 7.3 ± 0.2 @ 25°C |
*Contains papain
Preparation of Tryptone Soya Agar, Oxoid
First, you add 40g of tryptone soya agar to 1 litre of distilled water (purified as required). You can boil to dissolve the powder completely. After dissolved, you can sterilise the medium by autoclaving at 121°C for 15 minutes.
Storage Condition and Shelf Life of Tryptone Soya Agar, Oxoid
You can store the dehydrated tryptone soya agar at 10-30°C and use before the expiry date on the label or store the prepared plates of a medium at 2-8°C.
Tryptone Soya Broth (acc. to EP/USP)
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Tryptone Soya Broth is tested according to EP/USP.
Tryptone Soya Broth, Oxoid Composition
| Formula | gm/litre |
| Pancreatic digest of casein | 17.0 |
| Enzymatic digest of soya bean* | 3.0 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 2.5 |
| Glucose | 2.5 |
| pH 7.3 ± 0.2 @ 25°C |
*Contains papain
Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.
Tryptone Soya Broth 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Grow a wide variety of microorganisms with Thermo Scientific™ Oxoid™ Tryptone Soya Broth (a.k.a Tryptic Soy Broth) EP/USP/BP/JP (Dehydrated). The general purpose medium, containing two peptones, is suitable for the cultivation of both aerobes and anaerobes. It is specified in International Pharmacopoeia and food testing methods for the growth of a wide variety of organisms.
Tryptone Soya Broth, Oxoid Composition
| Formula | gm/litre |
| Pancreatic digest of casein | 17.0 |
| Enzymatic digest of soya bean* | 3.0 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 2.5 |
| Glucose | 2.5 |
| pH 7.3 ± 0.2 @ 25°C |
*Contains papain
Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.
Tryptone Soya Broth 5kg
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Tryptone Soya Broth (Tryptic Soy Broth) (Soybean Casein Digest Medium USP) is used for general laboratory use and sterility testing.
Tryptone Soya Broth, Oxoid Composition
| Formula | gm/litre |
| Pancreatic digest of casein | 17.0 |
| Enzymatic digest of soya bean* | 3.0 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 2.5 |
| Glucose | 2.5 |
| pH 7.3 ± 0.2 @ 25°C |
*Contains papain
Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.
Tryptone T 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Oxoid Tryptone T peptone developed with Lower ion Levels is ideal for the production of toxin by Clostridium tetani.
Tryptone Water 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Oxoid Tryptone Water is used for detection of indole production.
Tryptone Water, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 10.0 |
| Sodium chloride | 5.0 |
| pH 7.5 ± 0.2 @ 25°C |
Tryptone Water, Oxoid Preparation:
Dissolve 15g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.
Tryptose 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Tryptose is a mixed enzymatic hydrolysate used for the growth of many fastidious microorganisms.
Tryptose Blood Agar Base 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Cultivate and isolate fastidious microorganisms and determine their hemolytic reactions using Thermo Scientific™ Oxoid™ Tryptose Blood Agar Base (Dehydrated) with supplementation of blood. This highly nutritious medium is specially developed by Casman for the preparation of a blood agar which will support the growth of many fastidious organisms.
The original formulation included 0.3g dextrose per litre which interfered with the hemolytic reactions. It is now used without dextrose as a standard medium.
Tryptose Blood Agar Base (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptose | 10.0 |
| `Lab-Lemco’ powder | 3.0 |
| Sodium chloride | 5.0 |
| Agar | 12.0 |
| pH 7.2 ± 0.2 @ 25°C |
Tryptose Blood Agar Preparation:
Suspend 30g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
For blood agar, cool the basal medium to 45-50°C and add 7% of sterile blood. Mix thoroughly, taking care to avoid incorporation of air bubbles, and dispense into Petri dishes or other containers.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Tryptose Phosphate Broth 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Tryptose Phosphate Broth is recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms. Tryptose Phosphate Broth with added agar and sodium azide is recommended for the isolation of pathogenic streptococci from cheese and other dairy products.
Tube Racks
Login to see pricesBrand:
Biologix
This item is not available at the moment. Please contact us for more information.
Characteristics
● Made of high quality medical grade polypropylene
● 50-well racks hold 15ml centrifuge tubes
● 25-well racks hold 50ml centrifuge tubes
● Sturdy design keeps tubes upright
● 25-well racks hold 50ml centrifuge tubes’:
●Microtube racks hold 1.5/2ml microcentrifuge tubes
TWEEN® 20 (Sigma-Aldrich)
Login to see pricesBrand:
Sigma-Aldrich
Description
CAS number: 9005-64-5
Suitability: Suitable for DNA extraction, RNA extraction, cell culture and protein purification
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme-linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)
• emulsifying agent for the preparation of stable oil-in-water emulsions.
• pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• lysing mammalian cells at a concentration of 0.005 to 0.5%
TWEEN® 20 BioXtra (Sigma-Aldrich)
Login to see pricesBrand:
Sigma-Aldrich
Description
CAS number: 9005-64-5
Suitability: Suitable for blocking, cell culture, hybridization, protein purification, transformation
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 is a non-ionic detergent that has been used in a variety of applications, including:
- Post-hybridization wash buffer after in situ hybridization.
- Used along with PBS (phosphate-buffered saline) as the washing buffer for ELISA and immunostaining.
- Acted as an emulsifying agent for the preparation of stable oil-in-water emulsions
- Used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
- Acted as a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%.
- Used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
TWEEN® 20 Detergent (Merck)
Login to see pricesBrand:
Merck
Description
CAS number: 9005-64-5
Synonyms: Polysorbate 20, Polyoxyethylene (20) sorbitan monolaurate, PEG(20)sorbitan monolaurate
Form: Pale yellow liquid
Non-ionic detergent used for immunoprecipitation and for the solubilization of membrane-bound proteins.
Product Specification and MSDS for TWEEN® 20 Detergent (Merck).
TWEEN® 20 for Molecular Biology (Sigma-Aldrich)
Login to see pricesBrand:
Sigma-Aldrich
Description
CAS number: 9005-64-5
Suitability: Suitable for molecular biology
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 is a non-ionic detergent that widely used in biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%
• Along with PBS in the dilution of antibodies in immunohistochemistry technique
• For washing cells in FISH (Fluorescence in situ hybridization) technique
TZP110 Piperacillin/Tazobactam
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
TZP85 Piperacillin/Tazobactam
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Ub30 Flumequine
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Universal Beer Agar 500g
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
**This product is not available at the moment. Please contact us for more information.
Oxoid Universal Beer Agar is used for the isolation of beer spoilage microorganisms.
Universal Beer Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptonised milk | 15.0 |
| Yeast extract | 6.1 |
| Glucose | 16.1 |
| Tomato supplement | 12.2 |
| Dipotassium hydrogen phosphate | 0.31 |
| Potassium dihydrogen phosphate | 0.31 |
| Sodium chloride | 0.006 |
| Iron (II) sulphate † | 0.006 |
| Manganese (II) sulphate | 0.006 |
| Magnesium sulphate | 0.12 |
| Agar | 12.0 |
| pH 6.1 ± 0.2 @ 25°C |
Universal Beer Agar, Oxoid Preparation:
Suspend 62g in 750ml of distilled water and bring to the boil to dissolve completely. Add 250ml beer, without degassing, to the hot medium and mix gently. Distribute into final containers and sterilise by autoclaving at 121°C for 10 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.
Unstained Protein Standard, Broad Range (10-200 kDa)
Login to see pricesBrand:
New England Biolabs
This product is a direct replacement of NEB# P7704 (Unstained Protein Standard, Broad Range 10-200 kDa).
The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 or other commercially available, ready-to-use protein stains.
– Allows accurate molecular weight determination when performing SDS-PAGE analysis.
– Direct loading, additional loading buffer and heat incubation not required
– Recombinant proteins with no detectable protease contaminating activities
– Optimal stability for up to 24 months
Urea 40%
Login to see pricesBrand:
Thermo Scientific™ Oxoid™
- Oxoid Urea 40% Solution is used for addition to culture media.
- Each vial for 100mL medium.
Urea Agar Base 500g, Oxoid
Login to see pricesBrand:
Thermo ScientificTM OxoidTM
Oxoid Urea Agar Base (Christensen Agar Base) is used for detection of urease-producing microorganisms.
Urea Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 1.0 |
| Glucose | 1.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate | 1.2 |
| Potassium dihydrogen phosphate | 0.8 |
| Phenol red | 0.012 |
| Agar | 15.0 |
| pH 6.8 ± 0.2 |
Urea Agar Preparation:
Suspend 2.4g in 95ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C and aseptically introduce 5ml of sterile 40% Urea Solution SR0020. Mix well, distribute 10ml amounts into sterile containers and allow to set in the slope position.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
The alkaline reaction produced in this medium after prolonged incubation may not be caused by urease activity. Check using medium without urea.
Do not heat or reheat the medium because urea decomposes very easily.
For the detection of urease-positive Proteae the reaction must be read within the first 2-5 hours of incubation.
