DMEM with High Glucose with L-Glutamine 500ml
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Lonza
** This product has been discontinued.
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.
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DMEM with High Glucose with UltraGlutamine 500ml
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Lonza
** This product has been discontinued.
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.
The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.
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DMEM with High Glucose without L-Glutamine without Phenol Red 500ml
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Lonza
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.
The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The high glucose version is well suited to high density suspension culture.
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DMEM with Low Glucose without L-Glutamine 500ml
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Lonza
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The low glucose formula is used for adherent dependent cells.
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DNA Fingerprinting – Electrophoresis Education kit
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Cleaver Scientific
Our DNA fingerprinting kit is the perfect tool to introduce students to the world of electrophoresis and forensic science. DNA fingerprinting is a laboratory technique used to establish a link between biological evidence and a suspect in a criminal investigation. A DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA profiles are a match, then the evidence came from that suspect. Conversely, if the two DNA profiles do not match, then the evidence cannot have come from the suspect.
Each kit contains DNA, agarose gel, electrophoresis buffer and DNA stain enough for 8 groups of students to cast and run the experiment and visualise the DNA separation. The gel takes approximately 5 minutes to load, 30 minutes to run and 15 minutes to stain, meaning the whole experiment can be completed in a one hour lesson, with convenient incubation periods for scientific discussion and teaching. Students will solve a crime using an unknown DNA sample from the crime scene and a series of suspects!
Key Features
- Perfect for 1 hour lessons
- Introduce students to DNA and forensic science
- Works perfectly with Cleaver Scientific equipment
DNA Kit
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Cleaver Scientific
This simple kit will enable you to teach the principles behind gel electrophoresis. The kit contains five DNA samples labelled A to E, two of which are identical, with enough reagents to run two sets of six gels. The students will be able to run their own agarose gel and view the DNA after the samples have migrated through the gel. The DNA samples can be viewed on a standard UV transilluminator or using the totally sage blue light LED devices.
DNase agar 500g
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Thermo ScientificTM OxoidTM
Detect microbial deoxyribonuclease enzymes, particularly from staphylococci, with Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated). A DNase (deoxyribonuclease) is any enzyme that catalyzes the cleavage of phosphodiester linkages of DNA leading to its degradation. The organisms producing DNase enzymes in sufficient quantities to hydrolyze DNA will show clear zones around the colonies. The medium is mainly used in the identification of staphylococci but may also be used for the detection of DNase activity in other organisms.
DNASE Agar, Oxoid Composition
| Typical Formula* |
gm/litre |
| Tryptose |
20.0 |
| Deoxyribonucleic acid |
2.0 |
| Sodium chloride |
5.0 |
| Agar |
12.0 |
| pH 7.3 + 0.2 |
|
DNASE Agar, Oxoid Preparation:
Suspend 39g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Precautions
The DNase reaction for staphylococci is an indication of pathogenicity, it cannot be used as the sole criterion for identification.
Small zones of clearing may be caused by other enzymes or organic acid production.
Other organisms than staphylococci, Serratia and aeromonads can produce DNases.
Once the hydrochloric acid has been applied to the medium the plate must be read within a few minutes and further testing cannot be carried out by re-incubation.
The methyl green must be purified by extraction with chloroform.
Toluidine blue varies in performance according to source.
Merck Toluidine blue 1273 is satisfactory. Note that this dye cannot be used for Gram positive organisms.
DNase I (RNase-free)
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New England Biolabs
- Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends
- Degrades DNA template in transcription reactions
- Removes contaminating genomic DNA from RNA samples
- Isolated from a recombinant source
Do30 Doxycycline
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Thermo ScientificTM OxoidTM
Dor10 Doripenem 10µg
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Thermo ScientificTM OxoidTM
Dot Blotters
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Cleaver Scientific
Four different sample number, size and style of Hybridisation Manifold are offered; two types of Dot Blotter and two types of Slot Blotter. Typical applications include clone screening with DNA/RNA probes in Southern/Northern blots and immunological screening with antibodies in Western blots. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lateral transfer of samples and smudging by ensuring that a complete vacuum is formed. Six thumbscrews ensure even and tight sealing for fast sample suck down.
DPPH, Free Radical (Merck)
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Merck
Description
CAS number: 1898-66-4
Linear Formula: C₁₈H₁₂N₅O₆
Purity: ≥90% by elemental analysis
DPPH, Merck is a cell-permeable, stable free radical that acts as a hydrogen radical scavenger. Useful in inducing free-radical injury to tissues and as a screening tool for detecting free radical scavenging activity of antioxidants. Absorption max: ~517 nm. Protect the DPPH from light.
DRBC (ISO) Agar Base 500g
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Thermo ScientificTM OxoidTM
Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base while adhering to ISO 21527-1: 2008. Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar is specially designed to suppress the growth of spreading mold and bacteria while supporting the growth of species that cannot be isolated on Rose Bengal Chloramphenicol Agar or Acidified Potato Dextrose Agar.
Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 5.0 |
| Glucose | 10.0 |
| Potassium dihydrogen phosphate | 1.0 |
| Magnesium sulphate | 0.5 |
| Dichloran | 0.002 |
| Rose-bengal | 0.025 |
| Agar | 15.0 |
| pH 5.6 ± 0.2 @ 25°C |
Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Preparation:
Suspend 15.75g per 500ml (31.5g/l) of distilled water and heat to dissolve completely. Reconstitute Chloramphenicol Supplement (one vial SR0078E per 500ml or one vial SR0078H per 2 litres medium), as directed. Add the vial contents to the DRBC (ISO) Agar Base. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10–30°C and use before the expiry date.
Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).
DRBC Agar Base 500g
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Thermo ScientificTM OxoidTM
- Oxoid DRBC Agar Base (Dichloran Rose-Bengal Chloramphenicol Agar Base) is a selective medium for yeast and molds associated with food spoilage.
- Also available Chloramphenicol Selective Supplement, Part No. SR0078E and Chloramphenicol Selective Supplement, Part No. SR0078H
DRBC Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre |
| Peptone |
5.0 |
| Glucose |
10.0 |
| Potassium dihydrogen phosphate |
1.0 |
| Magnesium sulphate |
0.5 |
| Dichloran |
0.002 |
| Rose-Bengal |
0.025 |
| Agar |
15.0 |
| pH 5.6 ± 0.2 @ 25°C |
DRBC Agar, Oxoid Preparation:
Suspend 15.75g in 500ml (31.5g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the DRBC Agar Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).
Dry Bath Incubator
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Biologix
This product is not available at the moment. Please contact us for more information.
The Elite Series Dry Bath Incubators feature digital microprocessor control, user temperature calibration, and user-friendly design. One-block and dual-block types are available.
Features
• PID temperature control maintains temperature accuracy
• Leak-proof molded aluminum alloy chamber with PTFE coating
• Multi-functional: can also be used as a water bath or bead bath
• Various interchangeable heating blocks available
Heating block is not included, if you wish to purchase heating block, please click here.
Dry Bath Incubator
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Biologix
This item is not available at the moment. Please contact us for more information.
This classic dry bath incubator is ideal for molecular biology, histology, and environmental, industrial, and clinical laboratories, and for applications including melting and boiling point determinations, enzyme reactions, incubation and activation of cultures, immunoassays, DNA denaturation, culture media tests, coagulation tests, blood-urea-nitrogen determinations, and in-situ hybridizations.
Characteristics
• A broad temperature range, from room temperature to 120°C
• Continuous and timed operation settings
• Over-temperature protection
• Various interchangeable heating blocks are available
dsRNA Ladder – 25 gel lanes
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New England Biolabs
The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point.
This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels.
Dulbecco’s Modified Eagle’s Medium (DMEM), powder form
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Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins which suitable for cell culture. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Refer to the table below and choose your prefered DMEM!
duoview Transilluminator (21cm x 21cm)
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Cleaver Scientific
Product discontinued.
Our new duoVIEW transilluminator offers the user the added advantage of being able to view their gels with either standard UV light or using the safer blue light technology. This means only one transilluminatior need to be kept in the lab. They can either be used as a standalone unit or with the microDOC, as part of a fully integrated gel documentation system. With a large surface area, each transilluminator serves as the perfect workstation for viewing and working with fluorescently-stained protein and nucleic acid gels.
Dye Kit
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Cleaver Scientific
This simple kit will enable you to teach the principles behind gel electrophoresis without the need to view the samples on a UV or blue light transilluminator. The kit contains five dye samples labelled A to E with enough reagents to run two sets of six gels. Vial A contains a dye marker consisting all 4 dyes. The other vials B to E contain a single dye. When the samples are loaded into a gel and run on an electrophoresis unit the students will be able to watch the different coloured dyes separate by the size of the molecules, so simulating how DNA separates by different molecular weight.
E.C. Broth 500g
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Thermo ScientificTM OxoidTM
Selectively detect Escherichia coli from food and environmental samples with Thermo Scientific™ Oxoid™ EC Broth (Dehydrated). EC Broth is a selective medium for the differentiation of fecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples. It is also suitable for the enumeration of presumptive E. coli in milk and milk products using the most probable number technique.
EC Broth, Oxoid Composition
| Typical Formula* |
gm/litre |
| Tryptone |
20 |
| Lactose |
5.0 |
| Bile salts No. 3 |
1.5 |
| Di-potassium phosphate |
4.0 |
| Mono-potassium phosphate |
1.5 |
| Sodium chloride |
5.0 |
| pH 6.9 ± 0.2 @ 25°C |
EC Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Dispense into final containers and sterilize by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 4 weeks.
E.C. Broth with Mug 500g
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Thermo ScientificTM OxoidTM
Oxoid EC Broth with MUG is used for the isolation and presumptive identification of E. coli.
EC Broth with MUG, Oxoid Composition
| Typical Formula* |
gm/litre |
| Tryptone |
20.0 |
| Lactose |
5.0 |
| Bile salts No. 3 |
1.5 |
| Di-potassium phosphate |
4.0 |
| Mono-potassium phosphate |
1.5 |
| Sodium chloride |
5.0 |
| 4-methylumbelliferyl-ß-D-glucuronide (MUG) |
0.05 |
| pH 6.9 ± 0.2 @ 25°C |
|
EC Broth with MUG, Oxoid Preparation:
Suspend 37g of EC Broth with MUG in 1 litre of distilled water. Mix well to dissolve and distribute into final containers containing inverted Durham tubes. Sterilise by autoclaving at 121° C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Ensure that the glass tubes used for the preparation of the medium do not auto fluoresce and take the relevant safety precautions when using a UV lamp.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give a positive result in this test.
Other enteric organisms e.g. some Shigella species are also glucuronidase positive.
E.E. Broth 500g
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Thermo ScientificTM OxoidTM
Enrich Enterobacteriaceae in bacteriological examination of foods and animal feed stuffs with Thermo Scientific™ Oxoid™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated). This medium is more inhibitory to non-Enterobacteriaceae than other non-selective media e.g. Mannitol broth or Lactose broth by virtue of the presence of brilliant green and bile salts in the preparation.
EE Broth, Oxoid Composition
| Typical Formula* |
gm/litre |
| Peptone |
10.0 |
| Glucose |
5.0 |
| Disodium hydrogen phosphate anhyd. |
6.45 |
| Potassium dihydrogen phosphate |
2.0 |
| Ox Bile purified |
20.0 |
| Brilliant green |
0.0135 |
| pH 7.2 ± 0.2 @ 25°C |
EE Broth, Oxoid Preparation:
Add 43.5g to 1 litre of distilled water. Distribute 100ml quantities in 250ml flasks and heat at 100°C for 30 minutes only. Cool rapidly in cold running water. This medium is heat sensitive. DO NOT AUTOCLAVE.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Avoid overheating the medium, especially the double-strength broth.
E.Sak Isolation Agar Base 500g
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Thermo ScientificTM OxoidTM
Oxoid Cronobacter Sakazakii Isolation Agar (ISO) is a chromogenic medium for enrichment and isolation of Cronobacter spp. from milk and milk products in compliance with ISO/TS 22964: 2006.
E.Sak Isolation Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre |
| Pancreatic digest of casein† |
7.0 |
| Yeast extract |
3.0 |
| Sodium chloride |
5.0 |
| Sodium deoxycholate†† |
0.6 |
| 5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside |
0.15 |
| Crystal violet |
0.002 |
| Agar |
12.0 |
| pH 7.0 ± 0.2 @ 25°C |
E.Sak Isolation Agar, Oxoid Preparation:
Suspend 27.75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium is best used freshly prepared, but may be suitable for longer storage following in-house validation.
