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Oxoid Dextrose Bacteriological is a sugar suitable for inclusion in bacteriological culture media.

Oxoid Dextrose Tryptone Agar is used for detection and enumeration of thermophilic and mesophilic microorganisms in food.

Dextrose Tryptone Agar, Oxoid Composition

Dextrose Tryptone Agar, Oxoid Preparation:
Suspend 27g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
Incubation at 55°C must be carried out under humid conditions e.g. wrapped dishes or in a high humidity environment.

Oxoid Dextrose Tryptone Broth is used for detection and enumeration of thermophilic and mesophilic microorganisms in food.

Dextrose Tryptone Broth, Oxoid Composition

Dextrose Tryptone Broth, Oxoid Preparation:
Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared broth below 25°C.

Description

This DG 18 (Dichloran glycerol chloramphenicol) agar (base) is tested according to ISO 21527 and FDA-BAM GranuCult®. DG 18 Agar is suitable to enumerate and isolate xenophilic moulds from dried and semi-dried foods such as dried fruits, spices, confectionery, cereals, nuts, and dried meat and fish products.

Diagnostic Sensitivity Test Agar was developed in Oxoid as a dual purpose medium which would satisfy both diagnostic and susceptibility requirements.

Diagnostic Sensitivity Test Agar, Oxoid Composition

Diagnostic Sensitivity Test Agar, Oxoid Preparation:
Add 40 g to 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
For blood agar, cool the base to 50°C and add 7% of Defibrinated Horse Blood SR0050. Mix with gentle rotation and pour into Petri dishes (12 ml for a 9 cm dish) or other containers.
RECONSTITUTION AND MIXING SHOULD BE PERFORMED IN A FLASK AT LEAST 2.5 TIMES THE VOLUME OF MEDIUM TO ENSURE ADEQUATE AERATION OF THE BLOOD.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
Diagnostic Sensitivity Test Agar has reduced thymidine activity and this will affect its performance as a primary isolation medium.

• Oxoid Dichloran-Glycerol (DG18) Agar Base is a selective low water activity (aw) medium for xerophilic molds from dried and semi-dried foods.
• Also available Chloramphenicol Selective Supplement, Part No. SR0078E and Chloramphenicol Selective Supplement, Part No. SR0078H.

Dichloran-Glycerol (DG18) Agar Base, Oxoid Composition

Dichloran-Glycerol (DG18) Agar, Oxoid Preparation:
Suspend 15.75g in 500ml (31.5g/l) of distilled water and heat to dissolve completely. Add 110g per 500ml (220g/l) of Glycerol (Analytical Reagent grade). Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the DG18 Agar Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).