Shop

• Oxoid EC Broth, Reduced Bile Salts is a selective enrichment broth for the growth of Escherichia coli O157 from food and environmental samples.
• Also available Novobiocin Selective Supplement, Part No. SR0181E.

EC Broth (Reduced Bile Salts), Oxoid Composition

EC Broth (Reduced Bile Salts), Oxoid Preparation:
Dissolve 18.3g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool the EC Broth (Reduced Bile Salts) to 50°C and aseptically add the contents of 1 vial of Novobiocin Supplement SR0181, reconsituted as directed. Dispense into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
When stored as directed, the supplement remains stable until the expiry date printed on the label.

Oxoid Edwards Medium, Modified is used for selective isolation of Streptococcus agalactiae.

Edwards Medium, Modified, Oxoid Composition

Edwards Medium, Modified, Oxoid Preparation:
Suspend 41g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C, add 5-7% of sterile bovine or sheep blood, mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C. and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Egg Yolk Tellurite Emulsion is an emulsion of egg yolk containing potassium tellurite for use in Baird-Parker Medium CM0275 and Baird-Parker Medium (ISO) CM1127.

A modified medium requiring the addition of basic fuchsin to form Endo Agar.

• Oxoid Endo Agar is an APHA recommended medium for the confirmation of coliforms.
• Also available Basic Fuchsin Indicator, Part No. BR0050A.

Endo Agar Base, Oxoid Composition

Endo Agar, Oxoid Preparation:
Suspend 36g in 1 litre of distilled water. Add 6ml (or as directed by the supplier) of a 10% w/v solution of basic fuchsin BR0050 (50:50 ethanol/distilled water). Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. Plates should be stored in the dark to preserve their pale pink colour.

BASIC FUCHSIN IS A POTENTIAL CARCINOGEN AND CARE SHOULD BE TAKEN TO AVOID INHALATION OF THE POWDERED DYE AND CONTAMINATION OF THE SKIN.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
Weigh out the basic fuchsin (BR0050) in a fume cupboard and avoid inhalation of the powder or contamination of the skin.
Keep the prepared medium away from light to avoid photo-oxidation.
Endo Agar is quoted by the American Public Health Association as a `Standard Methods’ medium for use in water¹ and dairy products. Windle Taylor recommended the medium for the isolation and differentiation of coli-aerogenes bacteria from water.

Selectively enrich bile tolerant Gram-negative bacteria during microbiological examination of pharmaceutical products with Thermo Scientific™ Oxoid™ Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP).

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Composition

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Preparation:
Dissolve 25.97g in 1 litre of purified water. Mix well and distribute 100ml into 250ml flasks. Heat the medium at 100°C using free flowing steam for 30 minutes only. Cool rapidly in cold running water.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-25°C.

Detect, enumerate and differentiate the members of coliform group with Thermo Scientific™ Oxoid™ Eosin Methylene Blue Agar (Modified) Levine (Dehydrated). This versatile medium, modified by Levine is used for the differentiation of Escherichia coli and Enterobacteria aerogenes and also for rapid identification of Candida albicans. The medium is prepared to the formula specified by the APHA for the detection and differentiation of the coliform group of organisms.

Eosin Methylene Blue Agar (Modified) Levine, Oxoid Composition

Eosin Methylene Blue Agar (Modified) Levine, Oxoid Preparation:
Suspend 37.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidise the methylene blue (i.e. restore its blue colour) and to suspend the precipitate which is an essential part of the medium.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
Further tests are required to confirm the presumptive identity of organisms isolated on this medium. Some strains of Salmonella and Shigella species will not grow in the presence of eosin and methylene blue. Store the medium away from light to prevent photo-oxidation.