Microbiology

• Oxoid Ampicillin Selective Supplement is used for the isolation of Aeromonas hydrophila.
• Add to to Aeromonas Medium Base, Part No. CM0833B.

Ampicillin Selective Supplement, Oxoid Preparation:

Suspend 29.5g in 500ml of distilled water. Bring gently to the boil. DO NOT AUTOCLAVE. Cool to 50°C and aseptically add one vial of Ampicillin Selective Supplement SR0136 reconsituted as directed. Mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium 2-8°C.

• Oxoid Anaerobe Basal Agar is used for growth of fastidious and other anaerobes.
• Also available N-S Anaerobe Selective Supplement, Part No. SR0107B, for use for nonsporforming anaerobes and G-N Anaerobe Selective Supplement, Part No. SR0108B, for use for gram-negative anaerobes.

Anaerobe Basal Agar, Oxoid Composition

Anaerobe Basal Agar, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium for upto 3 weeks at 2-8°C.

Cultivate anaerobic microorganisms, particularly Bacteroides spp. and other fastidious anaerobes with Thermo Scientific™ Oxoid™ Anaerobe Basal Agar (Dehydrated). Anaerobe Basal Broth is formulated from a range of nutrients which have been selected to optimize the recovery and growth of the majority of anaerobic organisms of clinical importance.

Anaerobe Basal Broth, Oxoid Composition

Anaerobe Basal Broth, Oxoid Preparation:
Suspend 35.4g of Anaerobe Basal Broth in 1 litre of distilled water. Bring to the boil to dissolve. Distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Anaerobe Basal Broth should be stored tightly capped in the original container at 10-30°C. When stored as directed, the medium will remain stable until the expiry date printed on the label.

This product has been discontinued. The alternative to this product is Peptone Water.

Oxoid Peptone Water Andrade’s is a nutrient base containing Andrade’s indicator to which carbohydrates and other diagnostic reagents may be added for use in fermentation studies.

Peptone Water Andrade’s, Oxoid Composition

Peptone Water Andrade’s, Oxoid Preparation:
Suspend 15 g in 1 litre of distilled water. Mix well to dissolve, distribute into tubes or bottles, containing Durham tubes and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium in the dark at room temperature.

• Oxoid Arcobacter Broth Base is used for the selective growth of Arcobacter spp.
• Also available CAT Supplement, Part No. SR0174E and CCDA Selective Supplement, Part No. SR0155E and SR0155H.

Arcobacter Broth Base, Oxoid Composition

Arcobacter Broth Base, Oxoid Preparation:

Dissolve 12g of Arcobacter Broth in 500ml of distilled water. Sterilise at 121°C for 15 minutes and allow to cool to below 50°C

For use with CAT supplement (SR0174)

Add one vial per 500ml medium of CAT Selective Supplement reconstituted as directed. Dispense into sterile containers.

For use with CCDA supplement (SR0155)

Add one vial per 500ml medium of CCDA Selective Supplement reconstituted as directed. Dispense into sterile containers.

Inoculated plates should be incubated aerobically, at 30°C, for 24 hours.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Oxoid Azide Blood Agar Base is a selective media for the detection and isolation of streptococci and staphylococci from feces, sewage and other specimens.

Azide Blood Agar Base, Oxoid Composition

Azide Blood Agar Base Oxoid Preparation:
Suspend 30g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. For azide blood agar, cool to 45-50°C and add 5% of sterile blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared blood agar plates of medium at 2-8°C.

Precautions
Proteus and Escherichia species may not always be inhibited on the Edward’s formulation.
Always use a light inoculum for best selective results.
Anaerobic incubation will enhance haemolytic reactions.
Haemolytic reactions will not be typical on Packer’s modification of Azide Blood Agar Base. Streptococcus lactis will not grow on Packer’s modification with 5% sheep blood.
Read the section on Hazard Precautions for azide-containing media.

Oxoid Azide Dextrose Broth (Rothe Broth) is used for the detection of enterococciin water, sewage, and food.

Azide Dextrose Broth (Rothe Broth), Oxoid Composition

Azide Dextrose Broth (Rothe Broth), Oxoid Preparation:
Add 35.6g to one litre of distilled water for single strength broth or 71.2g for double strength broth. Heat gently to dissolve. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This product contains less than 1% azide and has low toxicity. However, when handling the powder, wear gloves, mask and eye protection. When washing azide products down sinks, use sufficient water to prevent accumulation of azide in the plumbing.

Selectively isolate and enumerate Bacillus cereus from food samples with Thermo Scientific™ Oxoid™ Bacillus cereus Selective Agar Base (Dehydrated). Bacillus cereus Selective Agar Base meets the requirements for a medium that is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants. The medium is also sufficiently diagnostic that colonies of Bacillus cereus are readily identified and confirmed by microscopic examination.

Bacillus cereus Selective Agar Base, Oxoid Composition

Bacillus cereus Selective Agar Base, Oxoid Preparation:
Suspend 20.5g in 475ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Polymyxin B Supplement (SR0099) reconstituted as directed, then add 25ml of sterile Egg Yolk Emulsion (SR0047). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and Polymixin B Supplement at 2-8°C. Use before the expiry date on the label.
The prepared medium may be stored at 2-8°C.

Precautions
On this medium Bacillus cereus is indistinguishable from Bacillus thuringiensis.
Identify Bacillus cereus by colony form, colour, egg yolk hydrolysis and confirm with cell and spore morphology.
Occasional strains of Bacillus cereus show weak or negative egg yolk reactions.