OEM SeaKem® LE Agarose
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Lonza
Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
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Applications
Content & Storage
OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5, 1L
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Merck
** This product has been discontinued.
OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.
It is suitable to be used in:
- Cell Biology
- Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
- Diluent for live cells staining
- Sterile solution preparation Microbiology Sample Preparation
- Diluent and rinsing reagent in Microbial Membrane Filtration
Key Specification:
- Sterile by USP Standard
- DNase, RNase, Protease-Free
- < 500 ppb Total Organic Carbon
- < 0.025 EU/ml Bacterial Endotoxin
OneTaq 2X Master Mix with Standard Buffer
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New England Biolabs
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT / GC content
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
OneTaq DNA Polymerase
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New England Biolabs
The ONE polymerase for your endpoint PCR needs. OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- 2X higher fidelity than Taq
- Ideal for routine, AT- or GC-rich templates
- Hot start and master mix versions available
OneTaq Hot Start 2X Master Mix with GC Buffer
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New England Biolabs
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- Obtain high yields with GC rich templates (>65%)
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
OneTaq Hot Start 2X Master Mix with Standard Buffer
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New England Biolabs
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT / GC content
- 2X higher fidelity than Taq
- Hot Start feature enables room temperature reaction setup
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
OneTaq Hot Start DNA Polymerase
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New England Biolabs
The ONE polymerase for your endpoint PCR needs
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step
- Robust yield with minimal optimization
- Ideal for routine, AT- or GC-rich templates
- Room temperature reaction setup
OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer
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New England Biolabs
OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases for use with routine and difficult PCR experiments.
• Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
• Obtain high yields with GC rich templates (>65%)
• Offered with inert tracking dyes for easy and direct loading of PCR products onto gels
OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer
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New England Biolabs
The ONE polymerase for your endpoint PCR needs
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- Obtain high yields across a wide range of AT / GC content
- Offered with inert tracking dyes for easy and direct loading of PCR products onto gels
OneTaq One-Step RT-PCR Kit – 30 rxns
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New England Biolabs
Easy and efficient One-Step RT-PCR
- Optimized reaction mix for cDNA synthesis and PCR amplification in one step
- Hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- Offered with Quick-Load® dyes for sample tracking and direct loading of PCR products onto gels
OneTaq Quick-Load 2X Master Mix with Standard Buffer
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New England Biolabs
The ONE polymerase for your endpoint PCR needs
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT- and GC- content
- 2X higher fidelity than Taq
- Master mix format contains inert tracking dye for easy and direct loading of PCR products onto gels
OneTaq Quick-Load DNA Polymerase
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New England Biolabs
Limited-time promotion! Buy M0509Sx5 (5 tubes of OneTaq Quick-Load DNA Polymerase, 100 units) for RM500 only! Before 13th November 2020!
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Includes green Quick-Load reaction buffer for easy and direct loading of PCR products onto gels
- 2X higher fidelity than Taq
- Compatible with standard Taq protocols
OneTaq® RT-PCR Kit
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New England Biolabs
Easy and efficient two-step RT-PCR
- Optimized reaction mixes for cDNA synthesis and PCR amplification
- Hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- OneTaq® DNA Polymerase delivers robust amplification across a wide range of templates
Paternity Testing – Electrophoresis Education kit
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Cleaver Scientific
In this experiment, DNA from a mother, child and 2 possible fathers is seperated by electrophoresis to determine the paternity of the child. The human DNA is simulated with a series of unqiue fragments that form a profile for each sample, allowing students to identify the father based on the mother and childs seperation profiles.
This kit includes DNA, Agarose, electrophoresis buffer and DNA stain for 8 student groups.
Key Features
- Perfect for 1 hour lessons
- Introduce students to DNA and genetics
- Works perfectly with Cleaver Scientific equipment
PCR Marker
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New England Biolabs
- Size range: 50 bp to 766 bp
- Value pricing
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
Phosphate Buffered Saline Powder, pH 7.4, For Preparing 1 L solutions, Sigma
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Sigma-Aldrich
Application
• Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
• It has been used for the suspension of bacterial cells.
• It has been used during the fixation and immunostaining procedures.
Reconstitution
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate-buffered saline (NaCl 0.138 M; KCl – 0.0027 M); pH 7.4, at 25 °C.
Phusion High-Fidelity DNA Polymerase
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust reactions – maximal success with minimal optimization
- Offered with multiple buffers for customized reaction setup for different DNA templates
Phusion High-Fidelity PCR Kit
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New England Biolabs
Ideal for high speed and high-performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific®Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust Reactions – maximal success with minimal optimization
- Kit includes everything needed for a PCR reaction, including controls
Phusion High-Fidelity PCR Master Mix with GC Buffer
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust reactions – maximal success with minimal optimization
- Master mix format with GC buffer ideal for long, difficult, or GC-rich DNA templates
Phusion High-Fidelity PCR Master Mix with HF Buffer
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity compared to Taq
- Robust reactions – maximal success with minimal optimization
- Master mix format with HF buffer ideal for a wide range of AT/GC content
Phusion Hot Start Flex 2X Master Mix
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Hot start formulation reduces non-specific amplification
- Master mix format for ease of use and optimized performance
Phusion Hot Start Flex DNA Polymerase
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust reactions with minimal optimization
- Hot start formulation enables room temperature reaction setup and reduces non-specific amplification
- High yields with shorter extension times
proBLUE DNA Stain
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Cleaver Scientific
Agarose electrophoresis of DNA requires a staining step in order to visualise the DNA fragments. This can be done safely using methylene blue solution. Methylene blue binds to negatively charged DNA making DNA visible as blue bands within agarose following electrophoresis. Methylene blue solution is provided as a 10x concentrate. To use, dilute 10x concentrate by mixing one part of methylene blue with nine parts of water (Eg. 100 ml of 10x concentrate added to 900 ml water).
Proteinase K, Molecular Biology Grade – 2 ml
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New England Biolabs
Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates.
- Highly characterized for more consistent performance
- Optimal activity and stability for up to 24 months
- Active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0.
- No detectable endonuclease, exonuclease, DNase or RNase contaminating activities
