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Modified Brucella Selective Supplement

Brand:

Thermo ScientificTM OxoidTM

Oxoid Modified Brucella Selective Supplement is for the cultivation and isolation of Brucella spp.

  • Alternative supplement containing natamycin as a replacement for cycloheximide
  • Add to Blood Agar Base No. 2, Part No. CM0271B or Brucella Medium Base, Part No. CM0169B
  • Each vial supplements 500mL of medium

Modified Brucella Selective Supplement, Oxoid Composition

Vial contents (each vial is sufficient for 500 ml of medium)
Polymixin B

2,500 IU

Bacitracin

12,500 IU

Natamycin

25.0 mg

Nalidixic acid

2.5 mg

Nystatin

50,000 IU

Vancomycin

10.0 mg

 

Modified Brucella Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml Brucella Medium Base, Columbia Agar Base or Blood Agar Base No.2 together with 5-10% v/v inactivated horse serum SR0035 and 1-5% w/v sterile solution of glucose, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Modified Karmali Selective Supplement

Brand:

Thermo ScientificTM OxoidTM

Oxoid Modified Karmali Selective Supplement is for a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42° C.

  • Alternative supplement containing amphotericin B as a replacement for cycloheximide
  • Add to Campylobacter Agar Base (Karmali), Part No. CM0935B
  • Each vial supplements 500mL of medium

Modified Karmali Selective Supplement, Oxoid Composition

Vial contents: (each vial is sufficient for 500ml of medium)
per vial
per litre
Sodium pyruvate

50.0mg

100.0mg

Cefoperazone

16.0mg

32.0mg

Vancomycin

10.0mg

20.0mg

Amphotericin B

5.0mg

10.0mg

 

Campylobacter Agar (Karmali), Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Campylobacter Agar Base (Karmali) CM0935 cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Modified Lauryl Sulphate Tryptose Broth

Brand:

Thermo ScientificTM OxoidTM

Selectively enrich Cronobacter spp. from milk and milk samples using Thermo Scientific™ Oxoid™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated). Modified Lauryl Sulphate Tryptose Broth Base was originally described by Guillaume-Gentil et al. for the selective enrichment of Cronobacter spp. The high salt concentration of the medium restricts the growth of other members of Enterobacteriaceae and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments.

Modified Lauryl Sulphate Tryptose Broth Base, Oxoid Composition

Typical Formula*

gm/litre

Sodium Chloride

34.0

Enzymatic digest of animal and plant tissue

20.0

Lactose

5.0

Potassium dihydrogen phosphate

2.75

Dipotassium hydrogen phosphate

2.75

Sodium lauryl sulphate

0.1

pH 6.8 ± 0.2 @ 25°C

 

Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Where possible the complete medium should be prepared on the day of testing and stored for no longer than 1 day at 0-5ºC

Modified Listeria Selective Supplement (Oxford)

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Listeria Selective Supplement (Oxford) is used for the detection of Listeria monocytogenes.
  • Add to Listeria Selective Agar Base, Part No. CM0856B.

Modified Listeria Selective Supplement (Oxford), Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)

per vial

per litre
Amphotericin B
5.0mg
10.0mg

Colistin sulphate

10.0mg

20.0mg

Acriflavine

2.5mg

5.0mg

Cefotetan

1.0mg

2.0mg

Fosfomycin

5.0mg

10.0mg

 

Modified Listeria Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to500 ml of sterilised Listeria Selective Agar Base (Oxford Formulation) CM0856, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Modified Preston Campylobacter Supplement

Brand:

Thermo ScientificTM OxoidTM

Oxoid Modified Preston Campylobacter Selective Supplement is for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.

  • Alternative supplement containing amphotericin B as a replacement for cycloheximide
  • Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
  • Each vial supplements 500mL of medium

Modified Preston Campylobacter Selective Supplement, Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)
per vial
per litre
Polymyxin B
2,500IU

5,000IU

Rifampicin
5.0mg

10.0mg

Trimethoprim
5.0mg

10.0mg

Amphoteracin B
5.0mg

10.0mg

 

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Modified Semi-Solid Rappaport Vassiliadis Medium

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium) is used for the detection of motile Salmonella spp. from food and environmental samples.
  • Also available MSRV Selective Supplement, Part No. SR0161E and Novobiocin Supplement, Part No. SR0181E.

Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium), Oxoid Composition

Typical Formula*

gm/litre

Tryptose

4.59

Casein hydrolysate

4.59

Sodium chloride

7.34

Potassium dihydrogen phosphate

1.47

Magnesium chloride (anhydrous)

10.93

Malachite green oxalate

0.037

Agar

2.7

pH 5.4 ± 0.2 @ 25°C

 

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Precautions
The basal medium is very hygroscopic. When handling the powder a face mask and gloves must be worn, refer to the material safety data sheet.

Modified Tryptone Soya Broth 500g

Brand:

Thermo ScientificTM OxoidTM

Selectively enrich and isolate Escherichia coli O157 from food or fecal samples with Thermo Scientific™ Oxoid™ Modified Tryptone Soya Broth (Dehydrated). The modifications to the original medium include an increase in the concentration of the buffer, dipotassium hydrogen phosphate and bile salts. The bile salts inhibit the growth of spore formers and enterococci but allow the growth of Escherichia coli.

Monarch DNA Gel Extraction Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify DNA from agarose gels with high yields.

  • Elute in as little as 6 μl.
  • Prevent buffer retention and salt carry-over with optimized column design.
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol.
  • Buffers and columns available separately.
  • Significantly less plastic used when compared with other kits.
  • Responsibly-sourced and recyclable packaging.

** Tips for using Monarch DNA Gel Extraction kit

Monarch PCR and DNA Cleanup Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify high-quality DNA from PCR and other enzymatic reactions.

  • Elute in as little as 6 μl.
  • Prevent buffer retention and salt carry-over with optimized column design.
  • Save time with fast, user-friendly protocols.
  • No need to monitor pH.
  • Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments.
  • Buffers and columns available separately.
  • Significantly less plastic used when compared with other kits.
  • Responsibly-sourced and recyclable packaging.

** Tips for using Monarch PCR and DNA Cleanup Kit

Monarch Plasmid Miniprep Kit

Brand:

New England Biolabs

The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of up to 20 μg of high quality plasmid DNA.

  • Elute in as little as 30 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Reduce hands on time with faster protocols and less spin time
  • Monitor completion of certain steps using colored buffer system
  • No need to add RNase before starting
  • Easily label columns using tab and frosted surfaces
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging
  • No hazardous materials fees

** Tips for using Monarch Plasmid Miniprep Kit

Monarch RNA Cleanup Kit (10 µg), NEB

Brand:

New England Biolabs

The Monarch RNA Cleanup Kit (10 µg) enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
  • Elute in ≥ 6 µl for concentrated RNA
  • 70-100% RNA recovery, even with inputs < 20 ng
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
  • Simplified workflow with a single wash buffer
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications

** Tips for using Monarch RNA Cleanup Kit

Monarch RNA Cleanup Kit (500 µg), NEB

Brand:

New England Biolabs

The Monarch RNA Cleanup Kit (500 µg), NEB enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.

  • Ideal for purification of synthesized RNA following high-yield in vitro transcription reactions (alternative to MEGAclear™)
  • Optimized for the cleanup of RNA after enzymatic treatments including DNase I, Proteinase K, labeling and capping
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Elute in ≥ 50 µl for concentrated RNA 70-100% RNA recovery
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Simplified workflow with a single wash buffer
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications, including microinjection and transfection.

** Tips for using Monarch RNA Cleanup Kit

Monarch® DNA/RNA Protection Reagent

Brand:

New England Biolabs

  • Preserves and stabilizes RNA and DNA
  • A component of the Monarch® Total RNA Miniprep Kit (NEB #T2010)
  • Supplied as a 2X concentrate
  • Supplied in 2 bottles, each containing 28 ml

Monarch® Genomic DNA Purification Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify high quality, high molecular weight gDNA from multiple sample types, all with one kit!

  • For use with a broad range of sample types, such as cells, blood, and tissues (including fatty and fibrous tissues)
  • Also works with tough to lyse samples (e.g., bacteria, yeast)
  • Suitable for clinically-relevant samples (e.g., saliva, cheek)
  • Obtain excellent yields of highly-pure DNA
  • Extremely low residual RNA contamination (typically <1%)
  • Isolates high molecular weight gDNA (peak size typically ≥ 50 kb)
  • Use DNA directly in downstream applications, including PCR, qPCR and NGS
  • Fast, user-friendly protocols with short lysis times and minimal hands-on time
  • Can also be used to clean up previously extracted gDNA
  • Includes RNase A
  • Kit components available separately

** Tips on how to maximize DNA yield using Monarch Genomic DNA Purification kit

Monarch® HMW DNA Extraction Kit for Cells & Blood

Brand:

New England Biolabs

  • For the extraction of HMW DNA from cells and blood
  • Isolate DNA into the Mb size range
  • Easily tune the size of isolated DNA by adjusting the agitation speed during lysis
  • Fast, user-friendly workflows (30 minutes for cells, 60 minutes for blood)
  • Obtain best-in-class yields of highly-pure and intact DNA
  • Effective RNA removal
  • Rapid and efficient elution
  • Includes RNase A and Proteinase K
  • Ideal for long-read sequencing applications

Monarch® HMW DNA Extraction Kit for Tissue

Brand:

New England Biolabs

  • For the extraction of HMW DNA from a variety of tissues, bacteria and other samples (yeast, insect, amphibian)
  • Isolate DNA into the Mb size range from soft organ tissues and bacteria
  • For some samples, the size of isolated DNA can be tuned by adjusting the agitation speed during lysis
  • Fast, user-friendly workflows (90 minutes for tissues and bacteria)
  • Obtain excellent yields of highly-pure and intact DNA
  • Rapid and efficient elution
  • Ideal for long-read sequencing applications
  • Includes RNase A and Proteinase K, as well as microtube pestles for convenient sample homogenization

Monarch® RNA Cleanup Kit (50µg), NEB

Brand:

New England Biolabs

The Monarch RNA Cleanup Kit (50 µg), NEB enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labelling, capping or in vitro transcription (IVT)
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Elute in ≥ 20 µl for concentrated RNA
  • 70-100% RNA recovery
  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
  • Simplified workflow with a single wash buffer
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications, including transfection

** Tips for using Monarch RNA Cleanup Kit

Monarch® Total RNA Miniprep Kit

Brand:

New England Biolabs

Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with Monarch Total RNA Miniprep kit!

  • For use with blood, cells and tissues
  • Also works with tough to lyse samples (bacteria, yeast, plant)
  • Effectively purifies total RNA of all sizes, including small RNAs >20 nt
  • Efficient genomic DNA removal (column and DNase I-based)
  • Contains Proteinase K for processing of tissues and blood samples
  • Includes RNA Protection Reagent for sample preservation
  • Excellent value
  • Kit components available separately

** You may also interest in RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit.

** Tips for using Monarch RNA Cleanup Kits 

MOPS-BLEB Base (BAX-17710676)

Brand:

Thermo ScientificTM OxoidTM

Thermo Scientific™ Oxoid MOPS-BLEB Broth Base is a medium for using with BAX Listeria species.

MRS (ISO) Agar 500g

Brand:

Thermo ScientificTM OxoidTM

Isolate and enumerate lactic acid bacteria from food with Thermo Scientific™ Oxoid™ MRS (ISO) Agar (DeMan, Rogosa and Sharpe) (Dehydrated) while adhering to ISO 15214: 1998. This medium is designed to support the growth of all strains of Lactobacilli, even the difficult and slow-growing strains of Lactobacillus brevis and Lactobacillus fermenti.

MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Composition

Typical Formula *
gm/litre
Enzymatic digest of casein

10

Meat extract

10

Yeast extract

4

Tri-ammonium citrate

2

Sodium acetate

5

Magnesium sulphate heptahydrate

0.2

Manganese sulphate tetrahydrate

0.05

Di-potassium hydrogen phosphate

2

Sorbitan mono-oleate

1.08

Glucose

20

Agar

12.4

TOTAL66.73g/L

 

MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Suspend 66.73g in 1 litre of distilled water. Boil to dissolve the medium completely. Dispense into tubes, bottles or flasks and sterilize by autoclaving at 121°C for 15 minutes. Selectivity can be altered by pH adjustment.

Storage conditions and Shelf life
Store the dehydrated medium at 10–30°C and use before the expiry date on the label.
Store the prepared plates at 2–8°C.

Precautions
Sterilization of this medium in some autoclaves, at 121°C for 15 minutes, may cause the medium in some cases to fall outside the specified pH limit of 5.7 ± 0.1. Adjustment of the medium in these rare cases is recommended using acetic acid or sodium hydroxide.

MRS Agar 500g, Oxoid

Brand:

Thermo ScientificTM OxoidTM

Oxoid MRS Agar (de Man, Rogosa, Sharpe) (Dehydrated) is used for the growth of Lactobacilli.

Composition of MRS Agar, Oxoid

Typical Formula *
gm/litre
Peptone

10.0

`Lab-Lemco’ powder

8.0

Yeast Extract

4.0

Glucose

20.0

Sorbitan mono-oleate

1ml

Dipotassium hydrogen phosphate

2.0

Sodium acetate 3H2O

5.0

Triammonium citrate

2.0

Magnesium sulphate 7H2O

0.2

Manganese sulphate 4H2O

0.05

Agar

10.0

pH 6.2 ± 0.2 @ 25°C

 

Preparation of MRS Agar, Oxoid

Suspend 62g of your MRS Agar in 1 litre of distilled water. You can boil to dissolve the powder completely. After a dissolve, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Conditions and Shelf Life of MRS Agar, Oxoid

You can store your dehydrated MRS Agar at 10-30°C and use before the expiry date on the label. You can store the prepared plates at 2-8°C.

MRSV Selective Supplement 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

Oxoid MRSV Selective Supplement is used for the detection of Salmonella spp. from food and environmental samples.

  • Add to MSRV Medium Base, Part No. CM0910B and Lysine Iron Agar, Modified (MLIA), Part No. CM0886B
  • Each vial supplements 500mL of medium

MRSV Selective Supplement, Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)
per vial
per litre
Novobiocin

10.0mg

20.0mg

 

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

MRVP Medium 500g, Oxoid

Brand:

Thermo ScientificTM OxoidTM

Oxoid MRVP Medium. A glucose-phosphate medium is used for the differentiation of the coli-aerogenes group by the Methyl Red and Voges-Proskauer tests.

MRVP Medium, Oxoid Composition

Typical Formula*
gm/litre
Peptone

7.0

Glucose

5.0

Phosphate buffer

5.0

pH 6.9 ± 0.2

 

MRVP Medium, Oxoid Preparation:
Add 17g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-6°C.

Precautions
The MR-VP reactions are only part of the tests required to identify organisms.
Each laboratory should standardise on the inoculum density, volume of broth and the test container size.
MR tests require a minimum incubation of 48 hours before the pH indicator is added.
When using Barritt’s reagents add a-naphthol first and KOH second; do not reverse this order.
Vaughn et al. warned of false positive VP reactions if the completed tests are left standing for over an hour.