Q5 Hot Start High-Fidelity 2X Master Mix
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New England Biolabs
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Q5 Hot Start High-Fidelity DNA Polymerase
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New England Biolabs
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) – 10 rxns
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New England Biolabs
The Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
- Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
- Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
- Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
Q5® High-Fidelity DNA Polymerase, NEB
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New England Biolabs
Q5® High-Fidelity DNA Polymerase, NEB sets a new standard for both fidelity and robust performance. With the highest-fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Features of Q5® High-Fidelity DNA Polymerase, NEB:
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Q5U® Hot Start High-Fidelity DNA Polymerase, NEB
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New England Biolabs
Q5U® Hot Start High-Fidelity DNA Polymerase is a modified version of Q5® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U® contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
- Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
- Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
- Generate higher assembly efficiency and improve accuracy in USER® cloning
- Enable room temperature setup with aptamer-based hot start formulation
- Utilize optimized protocols for recommended applications
Qd15 Quinupristin/Dalfopristin
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Thermo ScientificTM OxoidTM
Quercetin, ≥95% (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 117-39-5
Chemical Formula: C15H10O7
Molar Mass: 302.24g/mol
Synonyms: 2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one, 3,3′,4′,5,6-Pentahydroxyflavone
Application
• Quercetin has been used as an antioxidant which reversed the immunosuppressive effects of high glucose and hyperglycemic sera in type 2 diabetic patients.
• It has been used as a detoxifying phytochemical in Apis mellifera.
• It has been used as a positive control in DPPH (2,2- diphenyl-1-picryhydrazyl) radical scavenging assay. It has also been used for the preparation of calibration curve to determine total flavonoid content.
Quick CIP, NEB
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New England Biolabs
Quick CIP from NEB is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.
Features of Quick CIP, NEB:
- Rapid and irreversible heat inactivation eliminates the unwanted activity
- Improved storage stability versus native enzyme
- Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
- Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
- Less enzyme required (high specific activity), resulting in a lower cost per reaction
- No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
- Active on unincorporated dNTPs in PCR products – improve DNA sequencing and SNP analysis
- Recombinant for purity, consistency and value
Quick Ligation Kit
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New England Biolabs
The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C )
- Fast – 5 minutes for cohesive or blunt ends
- Convenient – ligation performed at room temperature
- Flexible – suitable for all common ligation reactions
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Quick-Load 1 kb DNA Ladder
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New England Biolabs
This product is available in Quick-Load Purple format – enjoy sharper bands, no UV shadow, and a lower price.
- Size range: 500 bp to 10 kb
- Convenient, ready-to-load format
- Formulated with bromophenol blue dye
- Easy to identify reference bands
Quick-Load 1 kb Extend DNA Ladder – 125 gel lanes
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New England Biolabs
- Size range: 0.5 kb to 48.5 kb
- Convenient, ready-to-load format
- Formulated with bromophenol blue dye
- Easy to identify reference bands
- Suitable for 125 gel lanes
Quick-Load 100 bp DNA Ladder
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New England Biolabs
This product is available in Quick-Load Purple format – enjoy sharper bands, no UV shadow, and a lower price.
- Size range: 100 bp to 1,517 bp
- Convenient, ready-to-load format
- Formulated with bromophenol blue dye
- Easy to identify reference bands
- Small size suitable for 125 gel lanes; large size suitable for 375 gel lanes
Quick-Load 1kb Plus DNA Ladder – 250 gel lanes
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New England Biolabs
This product was previously named the Quick-Load® 2-Log DNA Ladder. There have been no changes to product formulation or specifications.This product is available in Quick-Load Purple format – enjoy sharper bands, no UV shadow, and a lower price.
- Size range: 100 bp to 10 kb
- Convenient, ready-to-load format
- Formulated with bromophenol blue dye
- Easy to identify reference bands
- Suitable for 250 gel lanes
Quick-Load Purple 1 kb DNA Ladder – 125 gel lanes, NEB
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New England Biolabs
Quick-Load Purple 1kb DNA Ladder, NEB is a molecular weight DNA marker with one tracking dye. It is a pre-mixed and a ready-to-load DNA ladder, which provides convenience to you.
Features of Quick-Load Purple 1kb DNA Ladder, NEB:
- Convenient, ready-to-load format utilizing NEB’s proprietary purple loading dye
- Size range: 500 bp to 10 kb
- Sharp bands
- No UV Shadow
- Easy to identify reference bands
- Now supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Quick-Load Purple 100 bp DNA Ladder – 125 gel lanes
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New England Biolabs
Quick-Load® Purple 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.
- Convenient, ready-to-load format with NEB’s proprietary purple loading dye
- Size range: 100 bp to 1,517 bp
- Sharp bands
- No UV Shadow
- Easy to identify reference bands
- Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Quick-Load Purple 1kb Plus DNA Ladder (0.1-10.0 kb) – 250 gel lanes
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New England Biolabs
This product was previously named the Quick-Load® Purple 2-Log DNA Ladder. There have been no changes to product formulation or specifications.
- Convenient, ready-to-load format with NEB’s proprietary purple loading dye
- Size range: 100 bp to 10 kb (100 bp and 1 kb ladder in one!)
- Sharp bands
- No UV Shadow
- Easy to identify reference bands
- Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Quick-Load Purple 50 bp DNA Ladder – 250 gel lanes
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New England Biolabs
- Convenient, ready-to-load format with NEB’s proprietary purple loading dye
- Size range: 50 bp to 1,350 bp
- Sharp bands
- No UV Shadow
- Easy to identify reference bands
- Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- Suitable for 250 gel lanes
Quick-Load Purple Low Molecular Weight DNA Ladder – 125 gel lanes
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New England Biolabs
- Convenient, ready-to-load format with NEB’s proprietary purple loading dye
- Size range: 25bp to 766 bp
- Sharp bands
- No UV Shadow
- Easy to identify reference bands
- Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- Suitable for 125 gel lanes
Quick-Load Taq 2X Master Mix – 500 rxns
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New England Biolabs
The industry standard
- Exceptional value in terms of cost per unit
- 2X master mix format for easy reaction setup – just add template DNA and primers
- Offered with inert tracking dyes for easy and direct loading of PCR products onto gels
R.P.F. Supplement, Oxoid
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Thermo ScientificTM OxoidTM
Oxoid RPF Supplement (Rabbit Plasma Fibrinogen) is used for the isolation and enumeration of coagulase positive staphylococci from food.
- Use only with Baird-Parker Agar Base, RPF, Part No. CM0961B
- Each vial supplements 100mL of medium
RPF Supplement (Rabbit Plasma Fibrinogen), Oxoid Composition
| Vial contents (each vial is sufficient for 100ml of medium) | |
| Fibrinogen | 0.375 g |
| Rabbit plasma | 2.5 ml |
| Trypsin inhibitor | 2.5 mg |
| Potassium tellurite | 2.5 mg |
RPF Supplement Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90 ml of sterile Baird-Parker Agar Base (RPF) CM0961 cooled to 48°C. Mix well and use immediately.
Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.
R2A Agar 500g
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Thermo ScientificTM OxoidTM
Enumerate heterotrophic bacteria from drinking water samples with Thermo Scientific™ Oxoid™ R2A Agar (Dehydrated). This nutritionally reduced medium was specially designed to enhance the recovery of stressed and chlorine damaged bacteria from treated water samples. Stressed or injured organisms during water treatment are unable to grow on high nutrient media since faster-growing organisms outgrow the former. Therefore the use of a low nutrient medium like R2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.
R2A Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 0.5 |
| Proteose peptone | 0.5 |
| Casein hydrolysate | 0.5 |
| Glucose | 0.5 |
| Starch | 0.5 |
| Di-potassium phosphate | 0.3 |
| Magnesium sulphate | 0.024 |
| Sodium pyruvate | 0.3 |
| Agar | 15.0 |
| pH 7.2 ± 0.2 @ 25°C |
R2A Agar, Oxoid Preparation:
Suspend 18.1g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
R2A Agar should be stored tightly capped in the original container at 10-30°C. When stored as directed the medium will remain stable until the expiry date printed on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.
Raka-Ray Medium 500g
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Thermo ScientificTM OxoidTM
- Oxoid Raka-Ray Agar Base is a medium for the isolation of lactic acid bacteria in beer and brewing processes.
- Requires supplementation with phenylethanol, sorbitan mono-oleate, and cycloheximide Part No. SR0222C.
Raka-Ray Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 5.0 |
| Tryptone | 20.0 |
| Liver concentrate | 1.0 |
| Maltose | 10.0 |
| Fructose | 5.0 |
| Glucose | 5.0 |
| Betaine HCL | 2.0 |
| Diammonium hydrogen-citrate | 2.0 |
| L-Aspartic acid† | 2.5 |
| L-Glutamic acid†† | 2.5 |
| Magnesium sulphate. 7H2O | 2.0 |
| Manganese (II) sulphate. 4H2O | 0.66 |
| Potassium phosphate | 2.0 |
| N-Acetyl glucosamine | 0.5 |
| Agar | 17.0 |
| pH 5.4 ± 0.2 @ 25°C |
Raka-Ray Agar, Oxoid Preparation:
Suspend 77.1g in 1 litre of distilled water. Add 10ml of sorbitan mono-oleate and 7ml of 0.1% Cycloheximide Solution (SR0222). Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 3g of phenylethanol. Pour into sterile Petri dishes or distribute into 4ml volumes held at 55°C if the overlay technique is to be used.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Although the concentration of cycloheximide in the medium is below toxic levels, precautions should be observed as detailed under HAZARDS section.
RapID ANA II System 20/KT
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Thermo Scientific™ Remel™
Identify over 90 medically important anaerobes in just four hours using the Thermo Scientific™ RapID™ ANA II System, an identification system based on enzyme technology.
Requires:
RapID Spot Indole, Part No. R8309002, and RapID Inoculation Fluid, Part No. R8325102
RapID CB PLUS System 20/KT
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Thermo Scientific™ Remel™
Identify Corynebacterium and other gram-positive coryneform bacilli in just four hours using the Thermo Scientific™ RapID™ CB PLUS System, an identification system based on enzyme technology.
Requires:
RapID Spot Indole, Part No. R8309002, and RapID Inoculation Fluid, Part No. R8325102
