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• Oxoid Sorbitol MacConkey Agar is a selective differential medium for the detection of E. coli O157.
• Also available C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar Base, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C. Pour into sterile Petri dishes.

If required, reconstitute one vial of C-T supplement per 500ml medium, according to the instructions in the product leaflet. Aseptically add this to cooled medium before pouring into sterile Petri dishes

Storage conditions and Shelf life
Dehydrated Sorbitol MaConkey Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

• Oxoid Sorbitol MacConkey Agar w/BCIG is a selective medium containing the chromogenic substrate BCIG.
• Can be used with C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar w/BCIG, Oxoid Composition

Sorbitol MacConkey Agar w/BCIG, Oxoid Preparation:
Suspend 51.6g of SMAC with BCIG in 1 litre of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Soy Peptone is obtained by the hydrolysis of soya flour and complies with the USP specification.

Thermo Scientific™ Remel™ Sputasol is a liquifying agent for sputum samples.

• Each vial dilutes in sterile distilled water to make 100mL

Sputasol, Oxoid Composition

Sputasol, Oxoid Preparation:
Aseptically add the contents of one vial (7.5ml) to 92.5ml of sterile distilled water. Use the working solution immediately or store at 2-8°C for upto 48 hours only.

• Oxoid STAA Agar Base is used for the isolation of Brochothrix thermosphactafrom food samples.
• Also available STAA Selective Supplement, Part No. SR0151E and STA Selective Supplement, Part No. SR0162E.

STAA Agar Base, Oxoid Composition

STAA Agar, Oxoid Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add:

a) for STAA Medium the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

b) for STA Medium the contents of 1 vial of STA Selective Supplement SR0162 reconstituted as directed.
Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid STAA Selective Supplement is used for the isolation of Brochothrix thermosphacta from food samples.

• Add to STAA Agar Base, Part No. CM0881B
• Each vial supplements 500mL of medium

STAA Selective Supplement, Oxoid Composition

STAA Selective Medium Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in contact with skin.

Thermo Scientific™ Oxoid Standard Plate Count Agar (Plate Count Agar) is a standard medium corresponding to the APHA formulation used for milk, food and dairy products.

Standard Plate Count Agar (Plate Count Agar), Oxoid Composition

Standard Plate Count Agar (Plate Count Agar), Oxoid Preparation:
Suspend 23.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into bottles and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Staphylococcus Medium No.110 is a selective medium is used for isolation and differentiation of pathogenic Staphylococci.

Staphylococcus Medium No.110, Oxoid Composition

Staphylococcus Medium No.110, Oxoid Preparation:
Suspend 150g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Disperse the precipitate by gentle agitation before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Enterococcus faecalis may grow on this medium as tiny colonies with slight mannitol fermentation.
The high salt content in Staphylococcus Medium No.110 may interfere with the coagulase reaction. Always sub-culture to a non-inhibitory medium before testing.

Oxoid Streptococcus Supplement is used for the isolation of Streptococci.

• Add to Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Streptococcus Selective Supplement, Oxoid Composition

Streptococcus Selective Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500ml of sterile Columbia Blood Agar Base containing 5% Defibrinated Horse Blood SR0050 cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Stuart Transport Medium is a semi-solid non-nutritional transport medium for fastidious, pathogenic organisms.

Stuart Transport Medium, Oxoid Composition

Stuart Transport Medium, Oxoid Preparation:
Suspend 16g in 1 litre of distilled water. Bring to the boil to dissolve completely and dispense into screw-capped 7ml bottles. Fill each bottle to the brim, tighten the cap and sterilise by autoclaving at 121°C for 15 minutes. When sufficiently cool to handle, mix by inversion.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 15-25°C.

Precautions
A small amount of blue colour at the top of the bottle indicates oxidation. If this colour extends down into the medium it should be discarded.
Avoid prolonged heating in open flasks, during the preparation of the medium, because thioglycollate is volatile.
Sodium glycerophosphate may be metabolised by some organisms and thus promote their growth.