Buffer tablets pH 6.8, Merck
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Merck
Description
Buffer tablets with a pH of 6.8 are recommended for the preparation of buffer solutions according to WEISE for the staining of blood smears. These tablets are used to make buffer solutions and pH-buffered staining solutions, specifically phosphate buffer solutions, which are commonly used in hematology. They are also used to dilute staining solutions and rinse stained samples during the staining process.
100 buffer tablets are sufficient for preparing 100 liters of buffer solution, and each solution can remain stable for up to four weeks. The buffer tablets according to WEISE are in vitro diagnostic products that are CE registered.
Buffer tablets pH 7.2, Merck
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Merck
Buffer tablets pH 7.2 – for preparing buffer solution acc. to WEISE for staining of blood smears, are used to prepare buffer solutions according to WEISE and pH-buffered staining solutions. Phosphate buffer solutions are used in hematology, for the dilution of staining solutions and for the rinsing steps in the staining process. The buffer tablets acc. to Weise will produce a buffered solution (pH6.4/pH6.8/pH7.2) that stabilizes the staining result and gives reproducible staining results. Buffer solution acc. to Weise pH 6.4 results in bright-orange stained erythrocytes and differentiated stained cells with nuclei, whereby the erythrocytes are stained orange when using pH 6.8. Buffer solution acc. to Weise pH 7.2 produces grayish stained erythrocytes and intense red-violet stained cells with nuclei.
100 tablets are sufficient for 100 l buffer solution, each produced solution can be stable up to 4 weeks. Buffer tablets acc. to Weise are IVD products and CE registered.
Buffered Listeria Enrichment Broth 500g
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Thermo ScientificTM OxoidTM
Oxoid Buffered Listeria Enrichment is selective enrichment medium for the detection of Listeria monocytogenes.
Buffered Listeria Enrichment Broth, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone soya broth | 30.0 |
| Yeast extract | 6.0 |
| Potassium di-hydrogen orthophosphate | 1.35 |
| Disodium hydrogen orthophosphate | 9.60 |
| Final pH 7.3 ± 0.2 @ 25°C |
Buffered Listeria Enrichment, Oxoid Preparation:
Add 23.5g to 500ml of distilled water and mix well to dissolve. Add the contents of 1 vial of Listeria Selective Enrichment Supplement SR0141 or the alternative Modified Listeria Selective Supplement SR0213 reconstitued as directed. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and aseptically distribute into sterile containers in volumes as required.
Storage conditions and Shelf life
Buffered Listeria Enrichment Broth should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored as directed the medium will remain stable until the expiry date printed on the label.
Buffered Peptone Water (ISO) 500g
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Thermo ScientificTM OxoidTM
Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Buffered Peptone Water (ISO), Oxoid Composition
| Typical Formula* | gm/litre |
| Enzymatic digest of casein | 10.0 |
| Sodium chloride | 5.0 |
| Disodium hydrogen phosphate (anhydrous) | 3.5 |
| Potassium dihydrogen phosphate | 1.5 |
| pH 7.0 ± 0.2 @ 25°C |
Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Buffered Peptone Water (ISO) 5kg
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Thermo ScientificTM OxoidTM
Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Buffered Peptone Water (ISO), Oxoid Composition
| Typical Formula* | gm/litre |
| Enzymatic digest of casein | 10.0 |
| Sodium chloride | 5.0 |
| Disodium hydrogen phosphate (anhydrous) | 3.5 |
| Potassium dihydrogen phosphate | 1.5 |
| pH 7.0 ± 0.2 @ 25°C |
Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Buffered Peptone Water 2.5 kg
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Thermo ScientificTM OxoidTM
Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Buffered Peptone Water, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate | 3.5 |
| Potassium dihydrogen phosphate | 1.5 |
| pH 7.2 ± 0.2 @ 25°C |
Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.
Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.
Buffered Peptone Water 5 kg
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Thermo ScientificTM OxoidTM
Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Buffered Peptone Water, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate | 3.5 |
| Potassium dihydrogen phosphate | 1.5 |
| pH 7.2 ± 0.2 @ 25°C |
Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.
Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.
Buffered Peptone Water 500g
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Thermo ScientificTM OxoidTM
Buffered Peptone Water (Dehydrated), CM0509B (Oxoid) is a non-selective pre-enrichment medium that used to pre-enrich food samples prior to the isolation of Salmonella species. It also provides conditions for the resuscitation of cells that have been injured by processes of food preservation.
Buffered Peptone Water, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate | 3.5 |
| Potassium dihydrogen phosphate | 1.5 |
| pH 7.2 ± 0.2 @ 25°C |
Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.
Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.
BugBuster® Protein Extraction Reagent
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Merck
Description
Protein stability is fundamental to all aspects of protein research, including analysis by Western blotting. Combine our gentle protein extraction kits with protease inhibitors to obtain stabilized, intact and active proteins.
BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase®Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extraction from 20 g and 100 g cell paste.
Burkholderia Cepacia Agar Base 500g
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Thermo ScientificTM OxoidTM
Isolate Burkholderia cepacia from respiratory secretions of patients with cystic fibrosis with Thermo Scientific™ Oxoid™ Burkholderia Cepacia Agar Base, which is also used for routine testing of non-sterile inorganic salt solutions in hospital environments. The slower growing B. cepacia can be missed on conventional media, such as blood or MacConkey Agar, due to overgrowth caused by other faster growing organisms found in the respiratory tract of CF patients, such as mucoid Klebsiella species,
Pseudomonas aeruginosa and Staphylococcus species.This may lead to the infection being missed or wrongly diagnosed.
Burkholderia Cepacia Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 5.0 |
| Yeast Extract | 4.0 |
| Sodium pyruvate | 7.0 |
| Potassium dihydrogen phosphate | 4.4 |
| Disodium hydrogen phosphate | 1.4 |
| Bile salts | 1.5 |
| Ammonium sulphate | 1.0 |
| Magnesium sulphate | 0.2 |
| Ammonium ferrous sulphate | 0.01 |
| Phenol red | 0.02 |
| Crystal violet | 0.001 |
| Agar | 12.0 |
| pH 6.2 ± 0.2 @ 25°C |
Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Burkholderia Cepacia Selective Supplement
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Thermo ScientificTM OxoidTM
Oxoid Burkholderia Cepacia Selective Supplement is used for the isolation of Burkholderia cepacia.
- Add to Burkholderia Cepacia Agar Base, Part No. CM0995B
- Each vial supplements 500mL of medium
Burkholderia Cepacia Selective Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Polymixin B | 75,000IU | 150,000IU |
| Gentamicin | 2.5mg | 5.0mg |
| Ticarcillin | 50.0mg | 100.0mg |
Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Butadiene sulfone, 98% (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 77-79-2
Chemical Formula: C4H6O2S
Molar Mass: 118.15 g/mol
Synonyms: 2,5-Dihydrothiophene-1,1-dioxide, 3-Sulfolene
C L E D Medium 500g
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Thermo ScientificTM OxoidTM
Isolate, enumerate and differentiate urinary pathogens with Thermo Scientific™ Oxoid™ C.L.E.D. Medium (Dehydrated). Cystine-Lactose-Electrolyte-Deficient (CLED) Medium which provides good colonial differentiation, clear diagnostic characteristics and prevents swarming of Proteus spp. due to the electrolyte deficiency in the formulation.
C.L.E.D. Medium, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 4.0 |
| `Lab-Lemco’ powder | 3.0 |
| Tryptone | 4.0 |
| Lactose | 10.0 |
| L-cystine | 0.128 |
| Bromothymol blue | 0.02 |
| Agar | 15.0 |
| pH 7.3 ± 0.2 @ 25°C |
C.L.E.D. Medium, Oxoid Preparation:
Suspend 36.2g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°Cand use before the expiry date on the label.
Store the prepared medium at 2-8°C.
C10 Chloramphenicol
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Thermo ScientificTM OxoidTM
C30 Chloramphenicol
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Thermo ScientificTM OxoidTM
C50 Chloramphenicol
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Thermo ScientificTM OxoidTM
Cadmium Standard Solution, traceable to SRM from NIST (Merck)
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Merck
CAS no.: 7697-37-2, 10325-94-7
Traceability: Traceable to SRM from NIST Cd(NO₃)₂ in HNO₃ 0.5 mol/l 1000 mg/l Cd Certipur®
Synonym: –
Quality level: MQ300
Product specification and SDS for Cadmium Standard Solution.
Calcium chloride dihydrate, AR, 500g
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Chemiz
Description
Formula: CaCl2 · 2H2O
In cell culture laboratory, calcium chloride dihydrate is used as a calcium supplement in DMEM. It can also be used to prepare synthetic brine solution. In addition, it can also be used as a desiccant.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Calcium Standard Solution, traceable to SRM from NIST (Merck)
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Merck
CAS no.: 10124-37-5, 7697-37-2
Traceability: Traceable to SRM from NIST Ca(NO₃)₂ in HNO₃ 0.5 mol/l 1000 mg/l Ca Certipur®
Synonym: –
Quality level: MQ300
Product specification and SDS for Calcium Standard Solution.
CampyGen 2.5L (10 pack)
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Thermo ScientificTM OxoidTM
- Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with Oxoid CampyGen 2.5L Sachet.
- No water or catalyst required.
- No hydrogen gas produced.
- For AnaeroJar™ 2.5L, Part No. AG0025A.
Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.
Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.
CampyGen 3.5L (10/Pack)
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Thermo ScientificTM OxoidTM
Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with the Oxoid CampyGen 3.5L Sachet.
- No water or catalyst required
- No hydrogen gas produced
- For use with Thermo Scientific Oxoid 3.5L Anaerobic Jar, Part No. HP0011A, HP0031A
Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.
Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.
CampyGen Compact 20/Pck
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Thermo ScientificTM OxoidTM
- Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with the Oxoid CampyGen Compact Sachet.
- For use in Compact Plastic Pouch.
- Each pouch holds 2 standard size petri dishes.
- No water or catalyst required.
- No hydrogen gas produced.
- Use with Compact Plastic Pouch Part No. AG0020C and AnaeroGen™ Compact Sealing Clip, Part No. AN0005C.
Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.
Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.
Campylobacter Agar Base 500g
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Thermo ScientificTM OxoidTM
- Oxoid Campylobacter Agar Base is used for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian and environmental specimens.
- Also available Campylobacter Selective Supplement (Blaser-Wang), Part No. SR0098E NMKL; Bolton Broth Selective Supplement, Part No. SR0183E; Modified Bolton Broth Selective Supplement, Part No. SR0208E; Modified Preston Campylobacter Selective Supplement, Part No. SR0204E; and Campylobacter Selective Supplement Preston, Part No. SR0117E.
Campylobacter Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| `Lab-Lemco’ powder | 10.0 |
| Peptone | 10.0 |
| Sodium chloride | 5.0 |
| Agar | 12.0 |
| pH 7.5 ± 0.2 @ 25°C |
Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.
Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Campylobacter Growth Supplement (Liquid)
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Thermo ScientificTM OxoidTM
Oxoid Campylobacter Growth Supplement is a liquid supplement for the enhanced growth and aerotolerance of Campylobacter species.
- For the preparation of Blaser-Wang Medium, Bolton Selective Enrichment Broth, Butzler Selective Medium, and Preston Selective Medium
- Each vial supplements 500mL of medium
Campylobacter Growth Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500 ml of medium) | |
| Sodium pyruvate | 0.125 g |
| Sodium metabisulphite | 0.125 g |
| Ferrous sulphate (hydrated salt) | 0.125 g |
| Water | 2.0 ml |
Campylobacter Growth Medium, Oxoid Preparation:
Add the contents of one vial (2 ml) to 500 ml of a sterile nutrient medium cooled to 50-55°C prepared from Oxoid Columbia Agar, Blood Agar Base No.2, or Campylobacter Agar Base, with 5-7% lysed defibrinated horse or sheep blood, and the rehydrated contents of one vial of Campylobacter Antibiotic Supplement SR0069, SR0085 or SR0098. Mix gently and pour aseptically into sterile Petri dishes.
