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Thermo Scientific™ Oxoid Horse Serum is an extract of horse blood for addition to culture media.

Isolate Listeria spp. from food and environmental specimens using Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated). The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.

Fraser Broth Base, Oxoid Composition

Fraser Broth, Oxoid Preparation:
With Fraser Supplement:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

With Half Fraser Supplement:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers. Alternatively, to make 2.25 litres, use 129.0g of CM0895 and 1 vial of SR0166G.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Fraser Supplement is used for the isolation of Listeria species.

• Add to Fraser Broth Base, Part No. CM0895B
• Each vial supplements 500mL of medium

Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Selectively isolate Neisseria species with Thermo Scientific™ Oxoid™ GC Agar Base (Dehydrated). GC Agar Base has been formulated to include Special Peptone LP0072 which is a mixture of meat and plant enzymatic digests. The presence of starch in the medium ensures that toxic metabolites produced by Neisseria are absorbed. Phosphate buffers are included to prevent changes in pH due to amine production that would affect the survival of the organism

GC Agar Base, Oxoid Composition

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Oxoid G-N Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

G-N Anaerobe Selective Supplement, Oxoid Composition

Selective Medium for Gram-Negative Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of G-N Anaerobe Supplement (SR0108) rehydrated as directed, together with 25ml defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

This product has been discontinued. Please contact us for more information.

Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.

GBS Agar Base (Islam), Oxoid Composition

GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.