DMEM with High Glucose with Glutamine without Sodium Pyruvate 500ml
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Lonza
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.
The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The high glucose version is well suited to high density suspension culture.
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DMEM with High Glucose with L-Glutamine 1L
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Lonza
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.
The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.
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DMEM with High Glucose with L-Glutamine 500ml
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Lonza
** This product has been discontinued.
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.
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DMEM with High Glucose with UltraGlutamine 500ml
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Lonza
** This product has been discontinued.
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.
The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.
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DMEM with High Glucose without L-Glutamine without Phenol Red 500ml
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Lonza
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.
The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The high glucose version is well suited to high density suspension culture.
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DMEM with Low Glucose without L-Glutamine 500ml
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Lonza
Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The low glucose formula is used for adherent dependent cells.
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Dulbecco’s Modified Eagle’s Medium (DMEM), powder form
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Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins which suitable for cell culture. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Refer to the table below and choose your prefered DMEM!
Fetal Bovine Serum non-USA Origin, Sterile-filtered, Suitable for Cell Culture
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Sigma
Description
Synonyms: Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum
Fetal calf serum is a common supplement used in cell culture media to promote cell growth and proliferation. It is a non-USA origin fetal calf serum that has been sterile-filtered, making it free from contaminants and suitable for use in cell culture applications. This product is an excellent choice for those looking for a reliable and high-quality fetal calf serum that can support a wide range of cell types in culture.
MEM Eagle EBSS with L-Glutamine 1L
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Lonza
Product Overview
MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.
Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings.
EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.
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MEM Eagle EBSS with L-Glutamine 500ml
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Lonza
Product Overview
MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.
Amino acid concentrations conform closely to the protein composition of human cells.
Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.
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MEM Eagle EBSS without L-Glutamine 1L
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Lonza
Product Overview
MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.
Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.
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MEM Eagle EBSS without L-Glutamine 500ml
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Lonza
Product Overview
MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.
Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.
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Minimum Essental Medium Eagle (EMEM) with Joklik modification, powder form
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Sigma-Aldrich
EMEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. The formulation has been further modified by optional elimination of calcium to permit growth of cells in suspension culture.
Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form
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Sigma-Aldrich
This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!
Minimum Essential Medium Eagle (EMEM) with Earle’s Salt, powder form
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Sigma-Aldrich
Modification of original MEM formulation, which includes higher concentration of amino acids so the medium is more closely approximates the protein composition of cultured mammalian cells. EMEM has been used for wide variety of monolayer cells culture.
EMEM Modified with HEPES contains 25 mM HEPES to provide additional buffering capacity to the medium. A zwitterionic buffer, HEPES has a pKa of 7.3 at 37 °C, which is more compatible with most culture systems than that of sodium bicarbonate, which is usually 6.2 under similar conditions. HEPES will reduce sudden, drastic pH shifts, but as with other buffers, it will not prevent pH shifts entirely.
EMEM, which incorporates these modifications, includes 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Refer to the table below and choose your prefered EMEM!
Minimum Essential Medium Eagle (EMEM) with Hanks’ salts
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Sigma-Aldrich
Modification of the original MEM formulation. Earle’s salts have been replaced with Hanks’ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.
RPMI 1640 with L-Glutamine 500ml
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Lonza
Product Overview
RPMI 1640 Medium was developed for culturing normal human leukocytes. It can be used for suspension and monolayer mammalian cultures.
RPMI 1640 Medium will work with different cell lines, it can also be used with primary cells such as blood/hematopoietc cells.
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RPMI-1640 Medium
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Sigma-Aldrich
RPMI 1640 Medium, a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. Refer to the table below to choose the right RPMI-1640 for your cell culture!
RPMI-1640 Medium with 20 mM HEPES and L-glutamine, Sigma – Aldrich
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Sigma
Description
Form: Liquid
Synonyms: Roswell Park Memorial Institute 1640 medium
Techniques: Cell culture | mammalian: suitable
Impurities: Endotoxin Tested
pH: >7.2
Components:
HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes
RPMI 1640 Medium is a modification of McCoy’s 5A Medium designed to support lymphoblastoid cells in suspension culture. Although it was originally formulated to be used with a serum supplement, it has since been demonstrated to be capable of supporting a wide range of anchorage-dependent cells. RPMI 1640 Medium has also been extensively used in hybrid cell growth and fusion protocols. It has been shown to be suitable for the culturing of human normal and neoplastic leukocytes. This medium is based on the RPMI-1630 series of media and uses a bicarbonate buffering system with adjustments to amino acid and vitamin levels. It is also used for the growth of various cell cultures, such as fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.
TheraPEAK™ MSCGM™ CD Serum-free Mesenchymal Stem Cell Growth Medium BulletKit™
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Lonza
** This product has been discontinued.
Description
Stem cells possess the unique ability to proliferate as an undifferentiated cell type, then, when properly stimulated, differentiate into multiple lineages of cells. Lonza’s TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium is a chemically defined, serum-free, xeno-free media designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state. The medium has been specifically designed and tested to ensure that the medium neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media). Serum-free media, such as TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium, are intended to support cell growth without serum supplementation. This medium is chemically defined indicating that it contain only constituents of known molecular structure. Lonza’s TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium is of primary interest to those seeking maximal control over research and manufacturing variables. TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium’s formulation is complete and contains human albumin, recombinant human insulin, pasteurized human transferrin, HEPES, and L-glutamine. TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium does not contain phenol red or antibiotics. The basal medium (MSCBM-CD Mesenchymal Basal Medium, Chemically defined; catalog no.00190620) and the necessary supplements (MSCGM-CD™ SingleQuotsTMKit ; catalog no. 00192125) are included in this BulletKit.
Trypsin from Porcine Pancreas (Sigma)
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Sigma
Description
Product line: BioReagent
Biological source: Porcine pancreas
Specific activity: 1,000-2,000 BAEE units/mg solid
Trypsin is applicable for tissue disaggregation, due to its effective action and tolerance towards different cell types and serum-induced neutralization.[1]
X-VIVO™ 10 Serum-free Hematopoietic Cell Medium, xenofree
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Lonza
Description
The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVO™ 10 Media is available as a 1X liquid in two convenient formulations.
Key Applications:
-Proliferation of peripheral blood lymphocytes
-Proliferation of tumor infiltrating lymphocytes
-Cryopreservation of human tissue
-Cultivation of human monocytes and macrophages
-Cultivation of stem cells
-Cultivation of dendritic cells
X-VIVO™ 15, Serum free hematopoietic cell medium, xenofree
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Merck
Description
X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 Media and have been optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. X-VIVO™ 15 Media support the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells. In addition, X-VIVO™ 15 Media provide a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.
Applications:
-Proliferation of peripheral blood lymphocytes
-Proliferation of tumor infiltrating lymphocytes
-Cryopreservation of human tissue
-Cultivation of human monocytes and macrophages
-Cultivation of stem cells
-Cultivation of dendritic cells
-Cultiviation of CAR-T cells
X-VIVO™ 20 Serum-free Hematopoietic Cell Medium
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Lonza
Description
X-VIVO™ 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocytedepleted peripheral blood lymphocytes (PBL) at high density. Initial cell densities between 2.0 – 3.0 × 107 cells/ ml can be used to successfully generate LAK cells. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).
All BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures.
