Microbiology

• Oxoid Mycoplasma Agar Base is used for the selective isolation of Mycoplasma pneumoniae.
• Also available Mycoplasma Supplement P, Part No. SR0060C and Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Agar Base, Oxoid Composition

Mycoplasma Agar, Oxoid Preparation:
Add 35.5g to 1 litre of distilled water. Boil to dissolve the agar and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions in the HAZARDS section.
Do not use thallous acetate media to isolate Ureaplasma urealyticum.
Mycoplasmas may be suspected if (1) typical morphology (2) no growth in media without serum (3) colonies are embedded below the agar surface.

• Oxoid Mycoplasma Broth Base is used for the selective isolation of Mycoplasma species.
• Also available Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Broth Base, Oxoid Composition

Mycoplasma Broth, Oxoid Preparation:
Dissolve 25.5g in 1 litre of distilled water. Mix well and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions stated under Hazards section..
Sub-culture the broth to agar as soon as any indicator added begins to change colour before the pH change destroys the organism.

Oxoid Mycoplasma Supplement G is for the isolation of mycoplasmas.

• Add to Mycoplasma Pneumoniae Agar, Part No. CM0401B or CM0403B
• Each vial supplements 80mL of medium

Mycoplasma Supplement G, Oxoid Composition
POISON – CONTAINS THALLIUM SALT

Mycoplasma Medium Preparation:
Prepare the sterile supplement by aseptically adding 20ml sterile distilled water to the vial and mix gently. Add 1ml of Oxoid Mycoplasma Base without supplements to each of ten small bottles. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set. Aseptically add 2ml of the reconstituted Supplement-P to each bottle containing agar.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.

Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.

MYP Agar, Oxoid Composition

MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

This product is not available at the moment. Please contact us for more information.

Oxoid N-S Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

N-S Anaerobe Selective Supplement , Oxoid Composition

Selective Medium for Non-Sporing Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water containing 0.5ml `Tween 80’. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of N-S Anaerobe Supplement (SR0107) rehydrated as directed, together with 25ml of defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Oxoid Neomycin Selective Supplement (150mg/vial) allows the growth of clostridia and other anaerobes.

• Add to Blood Agar, Wilkins-Chalgren Anaerobe Agar, Part No. CM0619B, or Columbia Blood Agar, Part No. CM0331B
• Each vial supplements 2L of medium

Neomycin Selective Supplement, Oxoid Composition

Neomycin Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 2 litres of Wilkins Chalgren Agar Base CM0619 or another appropriate medium cooled to approximately 50° C. Mix well and pour into sterile Petri dishes.

Thermo Scientific™ Oxoid Nitrocefin is used for detection of beta-lactamase production.

• Reconstitutes to 500μg/mL
• Contents: 5 vials lyophilized nitrocefin (1mg) and 5 vials rehydrating fluid (1.9mL)

Oxoid Novobiocin Selective Supplement (10mg/vial) is added to EC Broth, Part No. CM0990B; Muller-Kauffmann Tetrathionate-Novobiocin Broth Base, Part No. CM0343B; Brilliant Green Agar, Part No. CM0263B; MRSV, Part No. CM0910B; Modified Tryptone Soya Broth, Part No. CM0989B; and Lysine Iron Agar, Modified, Part No. CM0886B.

• Each vial supplements 500mL of medium

Novobiocin Supplement, Oxoid Composition

Novobiocin Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to the designated medium cooled to approximately 50°C. Mix well and dispense into sterile containers.

Storage conditions and Shelf life
Novobiocin Supplement SR0181 should be stored in the dark at 2-8°C. When stored as directed, the supplement remains stable until the expiry date printed on the label.