Microbiology

Thermo Scientific™ Oxoid Tryptone Soya Broth (Tryptic Soy Broth) (Soybean Casein Digest Medium USP) is used for general laboratory use and sterility testing.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Oxoid Tryptone T peptone developed with Lower ion Levels is ideal for the production of toxin by Clostridium tetani.

Oxoid Tryptone Water is used for detection of indole production.

Tryptone Water, Oxoid Composition

Tryptone Water, Oxoid Preparation:
Dissolve 15g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Thermo Scientific™ Oxoid Tryptose is a mixed enzymatic hydrolysate used for the growth of many fastidious microorganisms.

Cultivate and isolate fastidious microorganisms and determine their hemolytic reactions using Thermo Scientific™ Oxoid™ Tryptose Blood Agar Base (Dehydrated) with supplementation of blood. This highly nutritious medium is specially developed by Casman for the preparation of a blood agar which will support the growth of many fastidious organisms.

The original formulation included 0.3g dextrose per litre which interfered with the hemolytic reactions. It is now used without dextrose as a standard medium.

Tryptose Blood Agar Base (Dehydrated), Oxoid Composition

Tryptose Blood Agar Preparation:
Suspend 30g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

For blood agar, cool the basal medium to 45-50°C and add 7% of sterile blood. Mix thoroughly, taking care to avoid incorporation of air bubbles, and dispense into Petri dishes or other containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

**This product is not available at the moment. Please contact us for more information.

Oxoid Universal Beer Agar is used for the isolation of beer spoilage microorganisms.

Universal Beer Agar, Oxoid Composition

Universal Beer Agar, Oxoid Preparation:
Suspend 62g in 750ml of distilled water and bring to the boil to dissolve completely. Add 250ml beer, without degassing, to the hot medium and mix gently. Distribute into final containers and sterilise by autoclaving at 121°C for 10 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.

• Oxoid Urea 40% Solution is used for addition to culture media.
• Each vial for 100mL medium.

Oxoid Urea Agar Base (Christensen Agar Base) is used for detection of urease-producing microorganisms.

Urea Agar Base, Oxoid Composition

Urea Agar Preparation:
Suspend 2.4g in 95ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C and aseptically introduce 5ml of sterile 40% Urea Solution SR0020. Mix well, distribute 10ml amounts into sterile containers and allow to set in the slope position.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The alkaline reaction produced in this medium after prolonged incubation may not be caused by urease activity. Check using medium without urea.
Do not heat or reheat the medium because urea decomposes very easily.
For the detection of urease-positive Proteae the reaction must be read within the first 2-5 hours of incubation.

Oxoid Urea Broth Base (Christensen Broth Base) is used for the detection of urease producing microorganisms.

Urea Broth Base, Oxoid Composition

Urea Broth Preparation:
Add 0.9 g to 95 ml of distilled water. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 55°C and aseptically introduce 5 ml of sterile 40% Urea Solution SR0020. Mix well and distribute 10 ml amounts into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is preferable that the medium be used on the day of preparation. If not, examine the tubes carefully to ensure sterility.
After overnight incubation other members of the Enterobacteriaceae may show alkaline reactions.

This product has been discontinued. Please contact us for more information.

Oxoid VCN Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

VCN Selective Supplement, Oxoid Composition

VCN Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Thayer Martin Medium, cooled to 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 plus the rehydrated contents of one vial of Vitox SR0090. Mix gently and pour into sterile Petri dishes.

Oxoid VCNT Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B

VCNT Selective Supplement, Oxoid Composition

VCNT Selective Medium Preparation:
Aseptically add 2ml of sterile distilled water to one vial mix gently to dissolve the powder completely. Aseptically add the contents of one vial to 500ml of sterile Thayer Martin Medium, cooled to approximately 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 and the rehydrated contents of one vial of Vitox SR0090.

Isolate and enumerate coliforms in food, animal feed and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), while adhering to ISO 4832:2006 and ISO/TS 11133-2:2003. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBL Agar (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBL Agar (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBL Agar (ISO) plates can be stored for up to 2 weeks when made from CM0968 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBL Agar (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBL Agar (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium
• Format: Freeze dried

Vancomycin Supplement, Oxoid Composition

Storage conditions and Shelf life
Vancomycin Supplement SR0186 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium

Vancomycin Supplement, Oxoid Composition

Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Thermo Scientific™ Oxoid Vegetable Peptone No. 1 is a highly nutritious, meat-free general purpose peptone for growth of bacteria and fungi.

Detect and enumerate Enterobacteriaceae in food products with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose Agar (Dehydrated). This glucose-containing selective medium gives reproducible counts of typical colonies for food safety analysis. In the examination of food stuffs, detection of the Enterobacteriaceae that ferment glucose to produce acid and/or gas has been recommended.

Violet Red Bile Glucose Agar (Dehydrated), Oxoid Composition

Violet Red Bile Glucose Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well and dispense into tubes or dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms may grow e.g. Aeromonas. The selective activity of this medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth. Medium for the pour-plate procedure should be freshly prepared, cooled to 47°C and used within 3 hours.