Microbiology

• Oxoid TTC Solution (0.125%) is used for the enumeration and identification of coliform bacteria.
• Add to Tergitol 7 Agar, Part No. CM0793B.
• Each vial for 100mL medium.

Thermo Scientific™ Oxoid T.T.C. Solution is a solution for the addition to KF Streptococcus Agar for the enumeration of entercocci.

T.T.C. Solution, Oxoid Composition

Medium Preparation:
Aseptically add the contents to 500ml of KF Streptococcus Agar CM0701 cooled to approximatly 50°C. Mix gently and pour into sterile Petri dishes.

Detect and enumerate Escherichia coli in food and animal feed samples with Thermo Scientific™ Oxoid™ Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated). The medium meets the formulation and performance criterion laid down in ISO 16649 and ISO 11133:2014.

TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Composition

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Preparation:
Suspend 36.6g of TBX Medium in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour the medium into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Oxoid Tergitol 7 Agar is used for the enumeration and identification of coliforms.

Tergitol 7 Agar, Oxoid Composition

TTC Solution (SR0148) is supplied as 2ml of filtered 0.125% aqueous solution of tri-phenyltetrazolium chloride (TTC).

Tergitol 7 Agar, Oxoid Preparation:
Suspend 54.15g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense in 100ml volumes and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the contents of 1 vial of SR0148. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Tergitol-7 Agar is designed for early detection of Escherichia coli i.e. 6-10 hours incubation.
Incubation at 44°C has been recommended.

Oxoid Tetrathionate Broth is used for the selective enrichment of Salmonella spp.

Tetrathionate Broth, Oxoid Composition

Tetrathionate Broth, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water and bring to the boil. Cool to below 45°C and add 20ml of iodine-iodide solution immediately before use. Mix continuously whilst dispensing 10ml volumes into sterile tubes. Use the complete medium (with added iodine) on the day of preparation.

The base may be prepared beforehand and kept for several weeks at 4°C. Iodine-iodide solution can then be added at the time of use to the quantity of medium needed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the base broth at 2-8°C. Use the medium immediately after adding the iodine solution.

Oxoid Tetrathionate Broth Base is used for selective enrichment of Salmonella species.

Tetrathionate Broth Base, Oxoid Composition
Add 77g to 1 litre of distilled water and bring to the boil. Cool below 45°C and add 20ml of iodine solution. Mix well and tube in 10ml quantities. The prepared base will keep for several weeks at 4°C but should be used soon after the addition of the iodine solution.

Iodine Solution

Tetrathionate Broth Preparation:
Tetrathionate Broth is recommended for the selective enrichment method of isolating Salmonella typhi and other salmonellae from faeces, sewage, etc.

Organisms which reduce tetrathionate, such as salmonellae, flourish in the medium whilst many faecal organisms are inhibited. Members of the Proteus group reduce tetrathionate and may consequently impair the value of this medium for the isolation of salmonellae; this disadvantage of the medium is largely overcome by the addition of 40µg of novobiocin to each millilitre of the incomplete medium before the addition of iodine.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium (without iodine solution) at 2-8°C.

A selective supplement for the detection, enumeration and presumptive identification of Listeria
monocytogenes and other Listeria species from food, animal feed and environmental samples
according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference
methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Directions:
Aseptically add 1 vial warmed to 48°C to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Selective Supplement (SR0257E) reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life

Store the selective (SR0257)  supplements at 2-8°C away from light. Ensure all product is used before the expiry date.

Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.

Brilliance Listeria Agar (ISO), Oxoid Composition

Brilliance Listeria Agar (ISO), Oxoid Preparation:

Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance™ Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance™ Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.

Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label

Brand:

Thermo Scientific^TM Oxoid^TM

A differential supplement for the detection, enumeration, and presumptive identification of Listeria monocytogenes and other Listeria species from food, animal feed and environmental samples according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Differential Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Differential Supplement, Oxoid Directions:
Aseptically add 5ml of sterile distilled water to 1 vial and mix gently to dissolve. Aseptically add the vial contents to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Differential Supplement (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the differential supplements (SR0258) at 2-8°C away from light. Do not freeze the differential supplement solution. Ensure all product is used before the expiry date.

A stabilised emulsion of egg yolk for use in culture media. It may be added directly to nutrient media for the identification of Clostridium, Bacillus and Staphylococcus species by their lipase activity.

Egg Yolk Emulsion, Oxoid Directions:
Suspend 21.5g of MYP Agar (CM0929) in 450ml of distilled water and bring gently to the boil to
dissolve. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and
aseptically add 50ml of Egg Yolk Emulsion (SR0047C) and the contents of one vial of Polymyxin B
Supplement (SR0099E) reconstituted as directed. Mix well and pour into sterile Petri dishes.
Alternatively, suspend 8g of Blood Agar Base (CM0055) in 200ml of distilled water and add 2g of
sodium chloride. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and
aseptically add 20ml Egg Yolk Emulsion (SR0047C). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Keep at temperatures between 2° and 8 °C. Use before expiry date on the label.

Precautions
On this medium Bacillus cereus is indistinguishable from Bacillus thuringiensis.
Identify Bacillus cereus by colony form, colour, egg yolk hydrolysis and confirm with cell and spore morphology.
Occasional strains of Bacillus cereus show weak or negative egg yolk reactions.

Thioglycollate Medium USP – Alternative, is intended for sterility testing with certain biological products that are turbid or otherwise do not lend themselves readily to culturing in Thioglycollate Medium USP (CM0173) because of its viscosity.

Thioglycollate Medium USP – Alternative, Oxoid Composition

Thioglycollate Medium USP – Alternative, Oxoid Preparation:
Suspend 29g in 1 litre of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilise by autoclaving at 121°C for 15 minutes.
PREPARE FRESHLY OR BOIL AND COOL THE MEDIUM JUST BEFORE USE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Whilst storage of the prepared medium is not recommended, autoclaved volumes of Thioglycollate Medium USP – Alternative, should be held at 20-30°C in the dark. Storage at lower temperatures increases oxygen absorption.

Precautions
This medium lacks agar and reducing indicator, therefore it is essential that the medium is freshly prepared and used within four hours of preparation.
Thioglycollate media should not be reheated more than once because toxic oxygen radicles are formed on reheating.

Cultivate aerobic and anaerobic organisms in the performance of sterility tests with Thermo Scientific™ Oxoid™ Thioglycollate Medium (USP) (Dehydrated). The medium is prepared according to the formula specified in the US Pharmacopoeia for the performance of sterility tests, and conforms to formulations detailed in the British and European Pharmacopoeia.

Thioglycollate Medium (USP) (Dehydrated), Oxoid Composition

Thioglycollate Medium (USP) (Dehydrated), Oxoid Preparation:
Suspend 29.75 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Distribute into final containers, sterilise by autoclaving at 121°C for 15 minutes. Mix well and cool to room temperature.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away from light at room temperature.

Precautions
If the upper portion of the medium is red because of oxidation, anaerobic conditions can be restored by reheating for 10 minutes in boiling water or steam. Do not reheat more than once.
Following reheating, if more than one-third of the medium is oxidised then it should be discarded.
Some glucose-fermenting organisms which are able to reduce the pH of the medium to a critical level may not survive in this medium. Early subculture is necessary to isolate these organisms.

Thermo Scientific™ Oxoid Thioglycollate Medium is tested according to EP/USP.

Thioglycollate Medium, Oxoid Composition

Thioglycollate Medium, Oxoid Preparation:
Suspend Thioglycollate medium (29.75 grams/ 1itre) in de-ionised water. Heat to dissolve. Cool and dispense 100ml into final containers, 100ml DIN bottles. Sterilise at 121°C for 15 minutes. When cooled label each bottle and pack in units of 10 into labelled boxes.

Storage conditions and Shelf life
Store away from light between 2-25ºC. Product shelf life from date of production is 1 year.