Shop

Oxoid Streptococcus Supplement is used for the isolation of Streptococci.

• Add to Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Streptococcus Selective Supplement, Oxoid Composition

Streptococcus Selective Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500ml of sterile Columbia Blood Agar Base containing 5% Defibrinated Horse Blood SR0050 cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Stuart Transport Medium is a semi-solid non-nutritional transport medium for fastidious, pathogenic organisms.

Stuart Transport Medium, Oxoid Composition

Stuart Transport Medium, Oxoid Preparation:
Suspend 16g in 1 litre of distilled water. Bring to the boil to dissolve completely and dispense into screw-capped 7ml bottles. Fill each bottle to the brim, tighten the cap and sterilise by autoclaving at 121°C for 15 minutes. When sufficiently cool to handle, mix by inversion.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 15-25°C.

Precautions
A small amount of blue colour at the top of the bottle indicates oxidation. If this colour extends down into the medium it should be discarded.
Avoid prolonged heating in open flasks, during the preparation of the medium, because thioglycollate is volatile.
Sodium glycerophosphate may be metabolised by some organisms and thus promote their growth.

Selectively isolate pathogenic Vibrio species with Thermo Scientific™ Oxoid™ TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated). The medium has shown to be the most satisfactory and reproducible formula regardless of the complex composition. Unlike Lauryl Sulphate Tellurite Agar it does not require further additives or aseptic additions of blood. It also possesses superior growth characteristics for Vibrio species, compared with tellurite media. While inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios after overnight incubation at 35°C.

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Composition

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE.
Pour plates without further heating and dry before use.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The identification of the various Vibrio species on TCBS Medium is presumptive and further tests are required for confirmation.
Yellow colonies on TCBS Medium will give unsatisfactory oxidase reactions.
Colonies taken from TCBS Medium are `sticky’ and react poorly in slide agglutination tests. Subculture to nutrient agar is required before slide agglutination tests can be carried out.
Some strains of Vibrio vulnificus produce better recovery at 30°C.

• Oxoid TTC Solution (0.125%) is used for the enumeration and identification of coliform bacteria.
• Add to Tergitol 7 Agar, Part No. CM0793B.
• Each vial for 100mL medium.

Thermo Scientific™ Oxoid T.T.C. Solution is a solution for the addition to KF Streptococcus Agar for the enumeration of entercocci.

T.T.C. Solution, Oxoid Composition

Medium Preparation:
Aseptically add the contents to 500ml of KF Streptococcus Agar CM0701 cooled to approximatly 50°C. Mix gently and pour into sterile Petri dishes.