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Enrich and isolate Salmonella from feces and food products with Thermo Scientific™ Oxoid™ Selenite Broth Base (Lactose).The presence of Selenium in the medium provides selectivity against non-target bacteria.

Selenite Broth Base (Lactose), Oxoid Composition

Selenite Broth Base (Lactose), Oxoid Preparation:
Dissolve 4g of sodium biselenite LP0121 in 1 litre of distilled water and then add 19g of Selenite Broth Base. Warm to dissolve, mix well and fill out into containers. Sterilise in a boiling water bath, or in free flowing steam, for 10 minutes. DO NOT AUTOCLAVE.

To minimise any possible risk of teratogenicity to laboratory workers, the sodium biselenite must be added as a solution to this medium.
Robertson1 reported miscarriages and possible teratogenic effects on pregnant laboratory assistants which may have been caused by ingested sodium biselenite. Oxoid therefore removed this substance from the powdered medium.

Although no further reports have been received sodium biselenite is now considered to be very toxic and should be handled with great care.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Discard the prepared medium if large amounts of reduced selenite can be seen as a red precipitate in the bottom of the bottles.
Do not incubate longer than 24 hours because the inhibitory effect of selenite is reduced after 6-12 hours incubation.
Take sub-cultures of broth from the upper third of the broth column, which should be at least 5 cm in depth.

Enrich and improve the isolation of salmonellae from feces and food samples with Thermo Scientific™ Oxoid™ Selenite Cystine Broth Base. This modified medium is based upon the formulation described by Leifson with added cystine.

Selenite Cystine Broth Base, Oxoid Composition

Selenite Cystine Broth, Oxoid Preparation:
Dissolve 4g of sodium biselenite LP0121 in 1 litre of distilled water and then add 19g of Selenite Cystine Broth Base. Warm to dissolve and dispense into containers. Sterilise by placing in free flowing steam for 15 minutes. DO NOT AUTOCLAVE.

Robertson reported miscarriages and possible tetragenic effects on pregnant laboratory workers which may have been caused by the ingestion of sodium biselenite. To minimise any possible risk of teratogenicity to laboratory workers the sodium biselenite is not included in the dry powder but should be prepared separately as a solution to which the Selenite Cystine Broth Base is added.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Observe the precautionary comments made about sodium biselenite in Selenite Broth Base CM0395.
Discard the prepared medium if large amounts of reduced selenite can be seen as a red precipitate in the bottom of the bottle.
Do not incubate longer than 24 hours because the inhibitory effect of selenite is reduced after 6-12 hours incubation.
Take subcultures of broth from the upper third of the broth column which should be at least 5 cm in depth.

This product has been discontinued. The alternative to this product is Sensitest Agar, 500g.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

Cultivate and isolate fastidious microorganisms from clinical samples through improved hemolytic reactions with sheep blood with Thermo Fisher™ Oxoid™ Sheep Blood Agar Base (Dehydrated) when used with sheep blood. Sheep Blood Agar Base was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of organisms without interfering with their hemolytic reactions when used with sheep blood.

Sheep Blood Agar Base (Dehydrated), Oxoid Composition

Sheep Blood Agar Base (Dehydrated), Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 7% sterile sheep blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.