SeaKem® LE Agarose
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Lonza
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID).
Benefits
Applications
Shrimp Alkaline Phosphatase (rSAP)
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New England Biolabs
Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source.
- Rapid and irreversible heat inactivation eliminates unwanted activity
- Improved storage stability versus native enzyme
- Faster reaction setup (no supplemental additives like zinc required)
- Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
- Less enzyme required (high specific activity), resulting in a lower cost per reaction
- No need for multiple phosphatases (rSAP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs )
- Active on unincorporated dNTPs in PCR products – improves DNA sequencing and SNP analysis
- Recombinant for purity, consistency and value
ssRNA Ladder – 25 gel lanes
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New England Biolabs
The ssRNA Ladder is a set of 7 RNA molecules produced by in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000, 1000 and 500 bases. The 3000 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing or native agarose gels.
Supercoiled DNA Ladder – 100 gel lanes
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New England Biolabs
The Supercoiled DNA ladder contains 9 proprietary supercoiled plasmids, ranging in size from 2 to 10 kb, that are suitable for use as supercoiled molecular weight standards for agarose electrophoresis. The 5 kb plasmid has an increased intensity to serve as a reference band.
- Recommended gel percentage range: 0.6-1%
- Optimum separation on 0.8%
- Recommended migration conditions: no more than 5V/cm (distance between electrodes), for no more than 60 minutes
T4 DNA Ligase, NEB
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New England Biolabs
Characteristics of T4 DNA Ligase, NEB:
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
- This enzyme will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
- T4 DNA ligase will seal nicks for these DNA substrates.
- The industry standard for performance and quality
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
TAE Buffer Solution
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
TAE buffer 50x solution: TAE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This 25x and 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.
Taq 2X Master Mix – 500 rxns
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New England Biolabs
Taq DNA Polymerase is the industry standard
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- 2X master mix format for easy reaction setup – just add template DNA and primers
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
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New England Biolabs
The industry standard
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- Mg-free buffer offers flexibility to adjust magnesium concentrations for use with microarray or DHPLC applications
Taq DNA Polymerase with Standard Taq Buffer, NEB
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New England Biolabs
Taq DNA Polymerase, NEB is a thermostable DNA polymerase that possesses 5′ to 3′ polymerase activity, that makes it an excellent industry standard for PCR.
Key features of Taq DNA Polymerase, NEB:
- Isolated from a recombinant source
- Robust and reliable reaction
- Incorporates dUTP, dITP and fluorescently -labeled nucleotides
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- Hot start version available
Taq DNA Polymerase, NEB is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
Taq DNA Polymerase with ThermoPol Buffer
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New England Biolabs
Taq DNA Polymerase is the industry standard for PCR
- Ideal for routine PCR applications
- ThermoPol Buffer delivers high product yield under demanding conditions
- Exceptional value in terms of cost per unit
Taq PCR Kit
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New England Biolabs
The industry standard
- Ideal for routine PCR applications
- Exceptional value in terms of cost per unit
- Kit format includes optimized reagents and enables flexible experimental design for 200 PCR reactions
TE Buffer
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
‘TE is deriving from ‘Tris’, a common pH buffer and ‘EDTA’, a cation chelators like Mg2+. TE buffer is normally used in molecular biology to solubilize DNA, RNA or cDNA while protecting it from degradation, or used in the cell culture. Here, we provide TE buffer in 10x or 100x with pH ranging from 7.4 to 8.
Thermostable RNase H, NEB
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New England Biolabs
Thermostable RNase H from NEB specifically recognizes and cleaves the phosphodiester bonds of an RNA strand in an RNA-DNA hybrid while leaving the DNA strand intact. This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures.
Features of Thermostable RNase H, NEB
- Recombinant enzyme supplied with 10X Reaction Buffer
- Does not digest single or double-stranded DNA
- Active and stable above 37°C over a broad temperature range, with activity increasing with temperature
- Requires magnesium chloride for activity
- Inactivated with proteinase K treatment or addition of excess EDTA
Trypsin-digested BSA MS Standard (CAM-modified) – 500 pmol
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New England Biolabs
This standard is a complex mixture of peptides produced by the tryptic digest of reduced and alkylated Bovine Serum Albumin (BSA).
- Used to standardize MALDI-TOF or ESI mass spectrometers (TOF, Q-TOF, Ion Trap, or Orbitrap)
- Standardization range of 500-2400 Da
- Free of salts, glycerol and detergents
- Supplied lyophilized
- Optimal stability for up to 24 months
Trypsin-ultra Mass Spectrometry Grade – 100 ug
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New England Biolabs
Trypsin-ultra, Mass Spectrometry Grade is a serine endopeptidase, which selectively cleaves peptide bonds C-terminal to lysine and arginine residues. Trypsin-ultra cleaves at Lys-Pro and Arg-Pro bonds at a much slower rate than when Lys and Arg are N-terminal to other residues.
- Analyze complex proteomes with minimal autolysis
- Compatible for simultaneous co-digestion with PNGase F (NEB# P0709)
- Compatible for simultaneous co-digestion with Endoproteinase LysC (NEB# P8109)
- Suitable for both in-gel and solution digests
- Supplied lyophilized
- Optimal activity and stability for up to 12 months
Unstained Protein Standard, Broad Range (10-200 kDa)
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New England Biolabs
This product is a direct replacement of NEB# P7704 (Unstained Protein Standard, Broad Range 10-200 kDa).
The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 or other commercially available, ready-to-use protein stains.
– Allows accurate molecular weight determination when performing SDS-PAGE analysis.
– Direct loading, additional loading buffer and heat incubation not required
– Recombinant proteins with no detectable protease contaminating activities
– Optimal stability for up to 24 months
WarmStart Colorimetric LAMP 2X Master Mix (DNA and RNA)
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New England Biolabs
Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
- Fast, clear pink-to-yellow visible detection of amplification
- WarmStart® feature inhibits activity at room temperature
WarmStart LAMP Kit (DNA and RNA)
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New England Biolabs
- Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
- Compatible with multiple detection methods
- WarmStart® technology inhibits activity at room temperature
