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Oxoid Wilkins-Chalgren Anaerobe Agar is used for the growth of anaerobes and recommended for antimicrobial susceptibility testing of anaerobes.

Wilkins-Chalgren Anaerobe Agar, Oxoid Composition

Wilkins-Chalgren Anaerobe Agar, Oxoid Preparation:
Suspend 43g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Wort Agar is a general purpose mycological medium, equivalent to the medium described by Parfitt and especially suitable for the cultivation and enumeration of yeasts. The medium which duplicates the composition of natural wort, is of an acidity which is optimal for many yeasts but inhibitory to most bacteria. Parfitt investigated the relative merits of wort agar and other media for the count of yeasts and moulds in butter, and recommended the use of dehydrated whey, malt or wort agar for the purpose. Scarr employed a modified wort agar (’osmophilic agar’) for the examination of sugar products for osmophilic yeasts. Scarr’s technique is also used for the determination of osmophilic yeasts occurring in materials used in the manufacture of soft drinks.

Wort Agar, Oxoid Composition

Wort Agar, Oxoid Preparation:
Suspend 50g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
PROLONGED OR EXCESSIVE HEATING WILL DIMINISH THE GEL STRENGTH OF THE AGAR.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not remelt the solid agar, it will destroy the gel. The surface of the agar is soft but suitable for poured inocula.

Isolate Salmonella and Shigella from clinical specimens and foods with selective Thermo Scientific™ Oxoid™ XLD Agar (Dehydrated). The medium relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria. The careful addition of sodium desoxycholate inhibits the growth of coliforms without decreasing the ability to support shigellae and salmonellae.

XLD Agar (Dehydrated), Oxoid Composition

XLD Agar (Dehydrated), Oxoid Preparation:
Suspend 53g in 1 litre of distilled water. Heat with frequent agitation until the medium boils. DO NOT OVERHEAT. Transfer immediately to a water bath at 50°C. Pour into sterile Petri dishes as soon as the medium has cooled.

It is important to avoid preparing large volumes which will cause prolonged heating.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Isolate Salmonella and Shigella from clinical specimens and foods with selective Thermo Scientific™ Oxoid™ XLD Agar (Dehydrated). The medium relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria. The careful addition of sodium desoxycholate inhibits the growth of coliforms without decreasing the ability to support shigellae and salmonellae.

XLD Agar (Dehydrated), Oxoid Composition

XLD Agar (Dehydrated), Oxoid Preparation:
Suspend 53g in 1 litre of distilled water. Heat with frequent agitation until the medium boils. DO NOT OVERHEAT. Transfer immediately to a water bath at 50°C. Pour into sterile Petri dishes as soon as the medium has cooled.

It is important to avoid preparing large volumes which will cause prolonged heating.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Efficiently isolate and identify salmonellae from clinical, environmental and food samples with highly selective Thermo Scientific™ XLT-4 Agar. XLT-4 Agar contains sodium tetradecylsulfate which acts to inhibit many of the background flora organisms whose growth can cause problems with differentiation on other Salmonellae plating media. This enhanced selectivity allows a significant increase in the recovery of salmonellae, while reducing false-positive suspect colonies, saving time and reducing the costs of further identification of false-positive colonies.

XLT-4 Agar Base, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid XLT-4 Selective Supplement is a selective supplement for use with XLT-4 Agar.

XLT-4 Selective Supplement, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid Yeast and Mold Agar is used for the isolation and maintenance of yeast and molds.

Yeast and Mold Agar, Oxoid Composition

Yeast and Mold Agar, Oxoid Preparation:
Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
For in vitro diagnostic use.
Do not use beyond the stated expiry date or if the product is caked, discoloured or shows any sign of deterioration.

Oxoid Yeast Autolysate Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

Yeast Autolysate Supplement, Oxoid Composition

Yeast Autolysate Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053. Mix gently and pour into sterile Petri dishes.

Oxoid Yeast Extract Powder is a dried yeast autolysate which provides a nutritious source of nitrogen and vitamins.

• Recommended for plate count of organisms in water
• Increases yield of organisms of many media or fermentation broths

Carry out plate counts of microorganisms in water with Thermo Scientific™ Oxoid™ Yeast Extract Agar (Dehydrated). Yeast Extract Agar is made to the formula described by Windle Taylor for the plate count of microorganisms in water and is tested in accordance with ISO 1133:2014. It is a rich, nutritious medium for the recovery of a wide range of bacteria, yeast, and molds.

Yeast Extract Agar, Oxoid Composition

Yeast Extract Agar, Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Yersinia Selective Agar Base (Schiemann’s CIN Agar) is used for the isolation and enumeration of Yersinia enterocolitica from clinical specimens and foods.
• Also available Yersinia Selective Supplement, Part No. SR0109E. Industrial Reference: FDA.

Yersinia Selective Agar Base, Oxoid Composition

Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.

Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.

Oxoid Yersinia Selective Supplement is used for the isolation of Yersinia enterocolitica.

• Add to Yersinia Selective Agar Base, Part No. CM0653B
• Each vial supplements 500mL of medium

Yersinia Selective Supplement, Oxoid Composition

Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.

Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.