Microbiology

Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with the Oxoid CampyGen 3.5L Sachet.

• No water or catalyst required
• No hydrogen gas produced
• For use with Thermo Scientific Oxoid 3.5L Anaerobic Jar, Part No. HP0011A, HP0031A

Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.

• Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with the Oxoid CampyGen Compact Sachet.
• For use in Compact Plastic Pouch.
• Each pouch holds 2 standard size petri dishes.
• No water or catalyst required.
• No hydrogen gas produced.
• Use with Compact Plastic Pouch Part No. AG0020C and AnaeroGen™ Compact Sealing Clip, Part No. AN0005C.

Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated) while adhering to BS ISO 21527-2: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), Oxoid Composition

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base, Oxoid Preparation:
Suspend 15.8g per 500ml (31.6g/l) of distilled water and heat to dissolve completely. Before autoclaving add 220g of Glycerol per 1 litre of medium. Heat the complete solution gently to aid its dissolution of the glycerol. This has been found to be a key procedure to follow to allow proper dissolution of the glycerol. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Where bacterial overgrowth occurs from certain foods (raw meats) chlortetracycline hydrochloride at 50mg/L (dissolved in water and filter sterilised) is recommended.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DG18 (ISO) Agar (CM1151) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated) while adhering to ISO 21527-1: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated), Oxoid Composition

Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Preparation:
Suspend 15.75g per 500ml (31.5g/l) of distilled water and heat to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes. It is advised to dispense the powdered medium into containers under an extraction hood due to the chloramphenicol in the medium base.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date

Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DRBC (ISO) Agar (CM1149) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.

Cultivate fastidious organisms with highly nutritious Thermo Scientific™ Oxoid™ Brain Heart Infusion Agar (Dehydrated). Brain Heart Infusion Agar is recommended for the cultivation of Streptococci, Neisseria and other fastidious organisms.

Brain Heart Infusion Agar, Oxoid Composition

Brain Heart Infusion Agar, Oxoid Preparation:
Suspend 47g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes. For blood agar, cool to 50°C and enrich with 10% v/v sterile defibrinated blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The medium is tested for compatibility using 7% v/v oxalated horse blood, defibrinated horse blood or defibrinated sheep blood. There should be no evidence of lysis or darkening after incubation at 37°C, 25°C or 4°C for 72 hours.
When using this medium to isolate Histoplasma capsulatum, Coccidioides immitis or other pathogenic fungi which can produce free infective spores, extreme care must be taken to avoid dissemination of infective particles in the laboratory. The cultures should be examined only in a closed, filtered air cabinet.

This medium is unsuitable for the screening of vancomycin resistant enterococci (VRE). More suitable and superior media for the screening of VRE are available; Brilliance VRE PO1226A or Remel BHI with Vancomycin RO1176.

Cultivate streptococci, Neisseria and other fastidious organisms with Thermo Scientific™ Oxoid™ Brain Infusion Broth (Dehydrated). The medium is highly nutritious and well buffered to support the growth of wide variety of organisms. It is recommended for blood culture work for the isolation and cultivation of pathogenic fungi.

Brain Infusion Broth, Oxoid Composition

Brain Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.