Microbiology

• Oxoid Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium) is used for the detection of motile Salmonella spp. from food and environmental samples.
• Also available MSRV Selective Supplement, Part No. SR0161E and Novobiocin Supplement, Part No. SR0181E.

Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium), Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Precautions
The basal medium is very hygroscopic. When handling the powder a face mask and gloves must be worn, refer to the material safety data sheet.

Enumerate heterotrophic bacteria from drinking water samples with Thermo Scientific™ Oxoid™ R2A Agar (Dehydrated). This nutritionally reduced medium was specially designed to enhance the recovery of stressed and chlorine damaged bacteria from treated water samples. Stressed or injured organisms during water treatment are unable to grow on high nutrient media since faster-growing organisms outgrow the former. Therefore the use of a low nutrient medium like R2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.

R2A Agar, Oxoid Composition

R2A Agar, Oxoid Preparation:
Suspend 18.1g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
R2A Agar should be stored tightly capped in the original container at 10-30°C. When stored as directed the medium will remain stable until the expiry date printed on the label.

The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Haemophilus Test Medium Base (HTM) is used for the susceptibility testing of Haemophilus influenzae.
• Also available HTM Supplement, Part No. SR0158E. Store at -20° to 0°C.

Haemophilus Test Medium Base (HTM), Oxoid Composition

Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
When stored as directed the supplement is stable until the expiry date stated on the label.

Oxoid Buffered Listeria Enrichment is selective enrichment medium for the detection of Listeria monocytogenes.

Buffered Listeria Enrichment Broth, Oxoid Composition

Buffered Listeria Enrichment, Oxoid Preparation:
Add 23.5g to 500ml of distilled water and mix well to dissolve. Add the contents of 1 vial of Listeria Selective Enrichment Supplement SR0141 or the alternative Modified Listeria Selective Supplement SR0213 reconstitued as directed. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and aseptically distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Buffered Listeria Enrichment Broth should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored as directed the medium will remain stable until the expiry date printed on the label.

Isolate Listeria spp. from food and environmental specimens using Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated). The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.

Fraser Broth Base, Oxoid Composition

Fraser Broth, Oxoid Preparation:
With Fraser Supplement:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

With Half Fraser Supplement:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers. Alternatively, to make 2.25 litres, use 129.0g of CM0895 and 1 vial of SR0166G.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Isolate and presumptively identify enterococci / Group D streptococci with Thermo Scientific™ Oxoid™ Bile Aesculin Agar (Dehydrated). Bile Aesculin Agar is designed to differentiate between Group D streptococci and non-Group D streptococci.

Group D streptococci hydrolyse aesculin to form aesculetin and dextrose. Aesculin hydrolysis is indicative of a positive result. Bile salts in the medium inhibit Gram-positive bacteria other than enterococci/ Group D streptococci.

Bile Aesculin Agar, Oxoid Composition

Bile Aesculin Agar, Oxoid Preparation:
Suspend 44.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

• Oxoid STAA Agar Base is used for the isolation of Brochothrix thermosphactafrom food samples.
• Also available STAA Selective Supplement, Part No. SR0151E and STA Selective Supplement, Part No. SR0162E.

STAA Agar Base, Oxoid Composition

STAA Agar, Oxoid Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add:

a) for STAA Medium the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

b) for STA Medium the contents of 1 vial of STA Selective Supplement SR0162 reconstituted as directed.
Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid PALCAM Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available PALCAM Selective Supplement, Part No. SR0150E or SR0150B.

PALCAM Agar Base, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

Precautions
Acriflavine hydrochloride is activated by light which may cause it to become inhibitory to Listeria growth.

Oxoid Azide Dextrose Broth (Rothe Broth) is used for the detection of enterococciin water, sewage, and food.

Azide Dextrose Broth (Rothe Broth), Oxoid Composition

Azide Dextrose Broth (Rothe Broth), Oxoid Preparation:
Add 35.6g to one litre of distilled water for single strength broth or 71.2g for double strength broth. Heat gently to dissolve. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This product contains less than 1% azide and has low toxicity. However, when handling the powder, wear gloves, mask and eye protection. When washing azide products down sinks, use sufficient water to prevent accumulation of azide in the plumbing.

Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated). Rappaport- Vassiliadis Soya Peptone Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated), Oxoid Composition

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated), Oxoid Preparation:
Suspend 26.75g in 1 litre of distilled water and heat gently to dissolve. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
RVS Broth should not be used if Salmonella Typhi is suspected.
In order to achieve optimum recovery it is recommended that the enrichment broth is incubated at 42 ± 1°C.

• Oxoid Listeria Enrichment Broth Base (UVM Formulation) is a two-step selective enrichment (USDA-FSIS) method.
• Also available Listeria Primary Enrichment Supplement-UVM I, Part No. SR0142E or SR0142B and Listeria Secondary Selective Enrichment Supplement-UVM II, Part No. SR0143E.
  Listeria Enrichment Broth Base (UVM formulation) is to be used with the appropriate supplement for UVMI broth or UVMII broth.

Listeria Enrichment Broth Base (UVM formulation), Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.

To Prepare Listeria Primary Selective Enrichment Medium (UVM I):
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

To Prepare Listeria Secondary Selective Enrichment Medium (UVM II):
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

• Oxoid Listeria Enrichment Broth is used for the enrichment of Listeria species.
• Also available Listeria Selective Supplement, Part No. SR0140E or SR0141E and Modified Listeria Selective Enrichment Supplement, Part No. SR0206E or SR0206B.

Listeria Enrichment Broth Base, Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 18g in 500ml of distilled water. Add the contents of one vial of Listeria Selective Enrichment Supplement (SR0141), reconstituted with 2ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140. Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against Listeria.
Supplement SR0141 used in this medium contains a toxic concentration of cycloheximide. Note the precautions to be taken under HAZARDS.

Oxoid Salmonella Rapid Test Elective Medium (SRTEM) is a selective enrichment broth medium specifically formulated for use with the Oxoid Salmonella Rapid Test Kit, Part No. FT0201A.

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Composition

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Preparation:
Add 61g to 1 litre of distilled water (double strength medium). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 18-25°C and use as described in the Oxoid Salmonella Rapid Test (Code FT0201) instruction leaflet.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

• Oxoid Listeria Selective Oxford Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available Listeria Selective Supplement, Part No. SR0140E and Modified Listeria Selective Supplement, Part No. SR0206E or SR0206B.

Listeria Selective Oxford Agar Base, Oxoid Composition

Listeria Selective Oxford Agar, Oxoid Preparation:
Suspend 27.75 of the Listeria Selective Agar Base (Oxford Formulation) in 500ml of distilled water. Bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Listeria Selective Supplement (Oxford Formulation) or Modified Listeria Selective Supplement (Oxford) SR0206, SR0140 reconstituted with 5ml of 70% ethanol. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Selectively detect Escherichia coli from food and environmental samples with Thermo Scientific™ Oxoid™ EC Broth (Dehydrated). EC Broth is a selective medium for the differentiation of fecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples. It is also suitable for the enumeration of presumptive E. coli in milk and milk products using the most probable number technique.

EC Broth, Oxoid Composition

EC Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Dispense into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 4 weeks.

Cultivate lactic streptococci and their bacteriophages with Thermo Scientific™ Oxoid™ M17 Broth (Dehydrated). The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine for bacteriophage assays.

M17 Broth, Oxoid Composition

M17 Broth, Oxoid Preparation:
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Sorbitol MacConkey Agar is a selective differential medium for the detection of E. coli O157.
• Also available C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar Base, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C. Pour into sterile Petri dishes.

If required, reconstitute one vial of C-T supplement per 500ml medium, according to the instructions in the product leaflet. Aseptically add this to cooled medium before pouring into sterile Petri dishes

Storage conditions and Shelf life
Dehydrated Sorbitol MaConkey Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Oxoid Tergitol 7 Agar is used for the enumeration and identification of coliforms.

Tergitol 7 Agar, Oxoid Composition

TTC Solution (SR0148) is supplied as 2ml of filtered 0.125% aqueous solution of tri-phenyltetrazolium chloride (TTC).

Tergitol 7 Agar, Oxoid Preparation:
Suspend 54.15g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense in 100ml volumes and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the contents of 1 vial of SR0148. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Tergitol-7 Agar is designed for early detection of Escherichia coli i.e. 6-10 hours incubation.
Incubation at 44°C has been recommended.

M17 Agar, Oxoid is used for the improved growth of Lactic Streptococci and their bacteriophages and the selective enumeration of Streptococcus thermophilus from yogurt.

M17 Agar, Oxoid Composition

M17 Agar, Oxoid Preparation:
Suspend 48.25g in 950ml of distilled water and bring gently to the boil. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2µm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid MLCB Agar is used for the selective isolation of Salmonella spp., not S.Typhi or S. Paratyphi A.

MLCB Agar, Oxoid Composition

MLCB Agar, Oxoid Preparation:
Suspend 49.0g in 1 litre of distilled water. Mix and bring gently to the boil with frequent agitation to dissolve the medium completely. Cool to 50°C and pour approximately 20ml into sterile Petri dishes.
DO NOT AUTOCLAVE OR OVERHEAT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The identity of colonies presumed from their appearance to be Salmonella spp. must be confirmed by biochemical and serological testing. In common with other enteric media care must be taken to ensure the purity of colonies taken for further testing as organisms that are inhibited from developing into colonies remain viable and may accidentally be picked on sub-culture.

• Oxoid Raka-Ray Agar Base is a medium for the isolation of lactic acid bacteria in beer and brewing processes.
• Requires supplementation with phenylethanol, sorbitan mono-oleate, and cycloheximide Part No. SR0222C.

Raka-Ray Agar Base, Oxoid Composition

Raka-Ray Agar, Oxoid Preparation:
Suspend 77.1g in 1 litre of distilled water. Add 10ml of sorbitan mono-oleate and 7ml of 0.1% Cycloheximide Solution (SR0222). Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 3g of phenylethanol. Pour into sterile Petri dishes or distribute into 4ml volumes held at 55°C if the overlay technique is to be used.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Although the concentration of cycloheximide in the medium is below toxic levels, precautions should be observed as detailed under HAZARDS section.

This product has been discontinued. Please contact us for more information.

Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.

GBS Agar Base (Islam), Oxoid Composition

GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.

Isolate Campylobacter jejuni, Campylobacter coli and Compylobacter laridis using Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) when used with CCDA Selective Supplement SR0155. Campylobacter Blood-Free Selective Agar Base is based on the original formulation described by Bolton et.al which was developed to replace blood with charcoal, ferrous sulphate and sodium pyruvate. For improved selectivity cephazolin in the formulation was replaced by cefoperazone as a selective agent.

Campylobacter Blood-Free Selective Agar Base, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Achieve maximum recovery of microorganisms during dilution with this protective, isotonic Thermo Scientific™ Oxoid™ Maximum Recovery Diluent (Dehydrated).

Maximum Recovery Diluent combines the protective effect of peptone in the diluting solution1 with the osmotic support of physiological saline. The low peptone concentration in the diluent reduces organism multiplication in the sample for at least 45 minutes during the dilution stage.

Maximum Recovery Diluent, Oxoid Composition

Maximum Recovery Diluent, Oxoid Preparation:
Dissolve 9.5g in 1 litre of distilled water. Dispense into the final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.