Microbiology

Oxoid Cronobacter Sakazakii Isolation Agar (ISO) is a chromogenic medium for enrichment and isolation of Cronobacter spp. from milk and milk products in compliance with ISO/TS 22964: 2006.

E.Sak Isolation Agar Base, Oxoid Composition

E.Sak Isolation Agar, Oxoid Preparation:
Suspend 27.75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium is best used freshly prepared, but may be suitable for longer storage following in-house validation.

Selectively enrich Cronobacter spp. from milk and milk samples using Thermo Scientific™ Oxoid™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated). Modified Lauryl Sulphate Tryptose Broth Base was originally described by Guillaume-Gentil et al. for the selective enrichment of Cronobacter spp. The high salt concentration of the medium restricts the growth of other members of Enterobacteriaceae and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments.

Modified Lauryl Sulphate Tryptose Broth Base, Oxoid Composition

Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Where possible the complete medium should be prepared on the day of testing and stored for no longer than 1 day at 0-5ºC

Isolate, enumerate, and identify coagulase positive Staphylococcus aureus in food samples with selective Thermo Scientific™ Oxoid™ Baird Parker Agar (ISO) Base (Dehydrated) while adhering to ISO 6888-1:1999.

Baird Parker Agar (ISO) Base, Oxoid Composition

Baird Parker Agar (ISO) Base, Oxoid Preparation:
Suspend 63g of Baird-Parker Agar (ISO) Base in 1,000ml of distilled water. Boil to dissolve the medium and sterilise by autoclaving at 121°C for 15 minutes. Cool to 47°C and aseptically add 50ml of Egg Yolk Tellurite Emulsion (SR0054). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium is best used freshly prepared, but may be suitable for longer storage following in-house validation.

Precautions
Atypical colonies have the same size as typical colonies but can be shining black with or without a narrow white edge; the clear zone or opacity halo can also be absent or barely visible. Grey colonies, free of clear zone, can also be regarded as atypical.
Regard all typical and atypical colonies as coagulase-positive staphylococci irrespective of negative reactions on the medium and carry out further tests.
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

• Oxoid Cronobacter Screening Broth Base (CSB) is a selective enrichment broth for isolation of Cronobacter spp, from food and environmental samples.
• Requires Vancomycin Supplement, 5mg, Part No. SR0247E.

Cronobacter Screening Broth Base (CSB), Oxoid Composition

Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Store the prepared medium at 2-8°C

Selectively enrich bile tolerant Gram-negative bacteria during microbiological examination of pharmaceutical products with Thermo Scientific™ Oxoid™ Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP).

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Composition

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Preparation:
Dissolve 25.97g in 1 litre of purified water. Mix well and distribute 100ml into 250ml flasks. Heat the medium at 100°C using free flowing steam for 30 minutes only. Cool rapidly in cold running water.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-25°C.

Selectively enrich Salmonella spp. from food, animal feces, and environmental samples with Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO) (Dehydrated). The efficacy of the medium is based upon the ability of Salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms and, thus, produce opaque halos of growth.

Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO), Oxoid Composition

MSRV (ISO) Agar, Oxoid Preparation:
Suspend 31.6g of MSRV (ISO) Agar base in 1 litre of distilled water. Bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool to 50°C, and aseptically add the contents of 1 vial of Novobiocin Supplement (SR0181), reconstituted as directed. Mix well, and pour into sterile Petri dishes. This medium is semi-solid, therefore do not invert plates.

Storage conditions and Shelf life
MSRV (ISO) should be stored in the tightly capped original container at 10-30°C. Novobiocin Supplement should be stored in the dark at 2-8°C. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Precautions
MSRV (ISO) Agar is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.
Basal medium is very hygroscopic.
When handling powder a face mask and gloves must be worn. Refer to material safety data sheet for details.

Easily isolate, enumerate, and presumptively identify common organisms associated with urinary tract infections using Thermo Scientific™ Brilliance™ UTI Clarity™ Agar. Organisms are differentiated through the utilization of two chromogenic substrates and/or tryptophan.

Brilliance UTI Clarity Agar, Oxoid Composition

Brilliance UTI Clarity Agar, Oxoid Preparation:
To prepare the medium from the base, suspend 37g of Brilliance UTI Clarity Agar (CM1106) in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121ºC for 15 minutes. Cool the medium to 50ºC and pour into sterile Petri dishes.

Storage conditions and Stability
Dehydrated Brilliance UTI Clarity Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Brilliance UTI Clarity Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM1106 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
Brilliance UTI Clarity Agar is for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration.

It should be noted that, as with all chromogenic media, organisms with atypical enzyme patterns may give anomalous reactions. The medium should be validated with locally prevalent strains to confirm sensitivity and specificity in the end-users hands, under local conditions.

A presumptive E. coli identification can be confirmed using DMAC indole reagent (do not use Kovac’s reagent as the colour of the pink colonies may be mistaken for the red colour of a positive indole result). The test should be performed on filter paper, not directly on the plate.

Sterilise specimens, equipment and media properly after use.

Thermo Scientific™ Brilliance Salmonella Agar Base is a chromogenic medium (formerly OSCM II) for isolation and presumptive identification of Salmonella spp. from food samples.

Brilliance Salmonella Agar Base, Oxoid Composition

 Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Brilliance Salmonella Agar should be stored in the tightly capped original container at 10-30ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Precautions
Brilliance Salmonella Agar is for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration.
Sterilise specimens, equipment and media properly after use.

Enrich and recover Salmonella spp. from food samples in a single step with Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Base, when prepared with Thermo Scientific Oxoid ONE Broth-Salmonella Selective Supplement (SR0242).

ONE Broth-Salmonella Base, Oxoid Composition

ONE Broth-Salmonella Base, Oxoid Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
ONE Broth-Salmonella should be stored tightly capped in the original container at 10-30ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
ONE Broth-Salmonella medium and Brilliance Salmonella Agar are for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration. Sterilize specimens, equipment and media properly after use; dispose waste in accordance with local rules and regulations. Directions should be read and followed carefully.

This product has been discontinued, please refer to CM1212B

Isolate, enumerate and differentiate Listeria species and Listeria monocytogenes from food samples with Thermo Scientific™ Oxoid™ Chromogenic Listeria Agar (ISO) (Dehydrated). Chromogenic Listeria Agar (ISO) contains lithium chloride, polymyxin B, nalidixic acid, and amphotericin which act to inhibit non-target organisms. A combination of chromogenic and phospholipase enzyme reactions easily differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).

Chromogenic Listeria Agar (ISO), Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This item is not available at the moment. Please refer to the alternative, Violet Red Bile Glucose Agar (CM0485B). However, please note that this product does not conform to ISO.

Selectively enumerate Enterobacteriaceae in food, animal feed, and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated) while adhering to ISO 21528 and ISO 11133:2014. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBGA (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBGA (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBGA (ISO) plates can be stored for up to 2 weeks when made from CM1082 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBGA (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBGA (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

This product has been discontinued, please refer to CM1212B
Brilliance Listeria Agar Base is a chromogenic medium for the isolation, enumeration, and presumptive identification of Listeria spp., including Listeria monocytogenes. Also available Chromogenic Listeria Selective Supplement, Part No. SR0227E and Chromogenic Listeria Differential Supplement, Part No. SR0228E.

Brilliance Listeria Agar Base, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between E. coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies.

ONE Broth-Listeria Base, Oxoid Composition

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Base must be stored tightly capped in the original container at 10-30°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Precautions
ONE Broth-Listeria Base and ONE Broth Selective Supplement are for Laboratory Use Only.
ONE Broth-Listeria Base is designated as “Irritant” and ONE Broth-Listeria Selective Supplement is designated as “Harmful”. For a full Material Safety Data Sheet (MSDS).
Do not use ONE Broth-Listeria Base or ONE Broth-Listeria Selective Supplement beyond the stated expiry dates, or if the products show any sign of deterioration.

Efficiently isolate and identify salmonellae from clinical, environmental and food samples with highly selective Thermo Scientific™ XLT-4 Agar. XLT-4 Agar contains sodium tetradecylsulfate which acts to inhibit many of the background flora organisms whose growth can cause problems with differentiation on other Salmonellae plating media. This enhanced selectivity allows a significant increase in the recovery of salmonellae, while reducing false-positive suspect colonies, saving time and reducing the costs of further identification of false-positive colonies.

XLT-4 Agar Base, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with Thermo Scientific™ Brilliance™ Cronobacter Sakazakii Agar (Dehydrated). A chromogenic substrate present in the medium detects a-glucosidase reaction in Cronobacter sakazakii. The enzyme a-glucosidase, present in Cronobacter sakazakii, hydrolyses the substrate producing blue-green colonies on this pale yellow medium.

Brilliance Cronobacter Sakazakii Agar, Oxoid Composition

Brilliance Cronobacter Sakazakii Agar, Oxoid Preparation:
Suspend 43.1g of Brilliance Cronobacter sakazakii Agar in 1 litre of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water (ISO), Oxoid Composition

Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Selectively isolate Salmonella species from food samples with Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn) for food testing in compliance with ISO 6579:2002.

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Composition

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Preparation:
Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and bring to the boil. Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution prepared by dissolving 25g of potassium iodide in 10ml of water, adding 20g of iodine and then diluting to 100ml with sterile water. Also add the contents of four vials of Oxoid Novobiocin Selective Supplement SR0181 reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at room temperature. The prepared medium in its final form containing iodine/iodide solution + Novobiocin Selective Supplement SR0181 should be used immediately.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between Escherichia coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies

Brilliance E. coli/coliform Selective Agar, Oxoid Composition

Brilliance E. coli/coliform Selective Agar, Oxoid Preparation:
Suspend 28.1g of Brilliance E. coli/coliform Selective Agar in 1 litre of distilled water. Bring the medium gently to the boil, to dissolve completely. Either pour the medium into sterile Petri dishes or keep molten at 45ºC for pour plate technique.

Storage conditions and Shelf life
Dehydrated medium: store tightly capped in the original container at 10-30°C
Prepared medium will be stable for up to 2 weeks when stored at 2-8ºC

Brilliance Bacillus Cereus Agar Base, Oxoid is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

• Also available Chromogenic Bacillus Cereus Selective Supplement, Part No. SR0230E.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

Oxoid Heart Infusion Broth is a highly nutritious liquid medium recommended for the cultivation of fastidious microorganisms.

Heart Infusion Broth, Oxoid Composition

Heart Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121ºC for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium in the dark below 20°C.

Precautions
Heart Infusion Broth should only be used for in vitro diagnostic purposes.

Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration with Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated). The presence of lauryl sulphate and lactose with phenol red allow the selective isolation and differentiation of Escherichia coli and coliforms respectively.

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Composition

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 week at 2-8°C.

Enrich and recover Vibrio species from food, water, and clinical samples with Thermo Scientific™ Oxoid™ Alkaline Peptone Water. This medium is selective by exploiting the ability of Vibrio species to tolerate alkaline conditions and high sodium chloride levels. The peptone content provides the conditions for growth and recovery of Vibrio species, supplying vitamins, minerals, and nitrogen in the form of amino acids.

Alkaline Saline Peptone Water, Oxoid Composition

Alkaline Saline Peptone Water, Oxoid Preparation:

Add 30g to 1 litre of distilled water. Mix well, distribute into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 month at room temperature.

Numerate all culturable microorganisms from water using colony count technique with Thermo Scientific™ Oxoid™ Water Plate Count Agar (Dehydrated). This nutritious, non-selective medium conforms to ISO 6222: 1999 (E) Water quality ― Enumeration of culturable microorganisms ― Colony count by inoculation in a nutrient agar culture medium1.

Water Plate Count Agar (Dehydrated), Oxoid Composition

Water Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 24g of Water Plate Count Agar (ISO) in 1 litre of distilled water and bring gently to the boil to dissolve. If desired, distribute volumes of 15ml to 20ml in tubes, bottles or other containers. Sterilise by autoclaving at 121°C for 15 minutes. After sterilisation, allow to cool and maintain at 45 ± 1°C using a water bath.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

• Oxoid Oxacillin Resistance Screening Agar Base (ORSAB) is a medium for screening clinical samples for the presence of oxacillin-resistant Staphylococci.
• Also available ORSAB Selective Supplement, Part No. SR0195E.

Oxacillin Resistance Screening Agar Base (ORSAB), Oxoid Composition

Oxacillin Resistance Screening Agar, Oxoid Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.