Microbiology

Thermo Scientific™ Oxoid Tryptone Soya Broth (Tryptic Soy Broth) (Soybean Casein Digest Medium USP) is used for general laboratory use and sterility testing.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Grow a wide variety of microorganisms with Thermo Scientific™ Oxoid™ Tryptone Soya Broth (a.k.a Tryptic Soy Broth) EP/USP/BP/JP (Dehydrated). The general purpose medium, containing two peptones, is suitable for the cultivation of both aerobes and anaerobes. It is specified in International Pharmacopoeia and food testing methods for the growth of a wide variety of organisms.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Oxoid Tryptone Glucose Extract Agar is a standard method medium for examination of water and dairy products.

Tryptone Glucose Extract Agar, Oxoid Composition

Tryptone Glucose Extract Agar, Oxoid Preparation:
Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. When the dilution of the original specimen is greater than 1 in 10, add 10 ml of sterile 10% solution of Skim Milk Powder LP0031 per litre.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Charcoal Agar Base (Bordetella Selective Medium) is used for selective isolation of B. pertussis and B. parapertussis.
• Requires addition of Bordetella Selective Supplement, Part No. SR0082E.

Charcoal Agar Base, Oxoid Composition

Charcoal Agar, Oxoid Preparation:
Suspend 51g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, add 10% of defibrinated blood and mix gently. The medium is made selective for the isolation of Bordetella pertussis and Bordetella parapertussis by the addition of Bordetella Selective Supplement SR0082.

To one vial add 2ml of sterile distilled water and dissolve the contents completely. Add this solution to 500ml of sterile, molten Charcoal Agar, cooled to 50°C, together with 10% v/v defibrinated horse blood SR0050. Mix well before pouring into sterile Petri dishes.
For Haemophilus influenzae, omit the selective agents and convert to `chocolate’ agar.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Stuart’s transport medium or similar formulation media should not be used for Bordetella-containing specimens.
Two pernasal swabs should be taken from each patient, one through each nostril.
Make sure the charcoal remains in suspension when dispensing the medium by gently swirling the flask.
Lysed horse blood is used in the transport medium but whole blood is used in the isolation medium.
Most naso-pharyngeal flora are inhibited by cephalexin but Pseudomonas aeruginosa and some fungi may grow through. Amphotericin B can be added (12mg/ml) as an antifungal agent to prevent the growth of filamentous fungi. However, this level of amphotericin B can be inhibitory to Bordetella pertussis and should not be used routinely.

Detect and enumerate coliform organisms, including Escherichia coli, while isolating and detecting Salmonella and Shigella spp. in both clinical and food specimens using Thermo Scientific™ Oxoid™ MacConkey Agar No.3. A modification of the original MacConkey medium by the addition of selected bile salts, the medium gives improved differentiation of coliforms and non-lactose fermenting organisms while completely inhibiting Gram-positive cocci.

MacConkey Agar No.3, Oxoid Composition

MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium at 2-8°C.

**This product is not available at the moment. Please contact us for more information.

Oxoid Tomato Juice Agar is used for cultivation of lactobacilli.

Tomato Juice Agar, Oxoid Composition

Tomato Juice Agar, Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the plates of prepared medium at 2-8°C.

Oxoid Stuart Transport Medium is a semi-solid non-nutritional transport medium for fastidious, pathogenic organisms.

Stuart Transport Medium, Oxoid Composition

Stuart Transport Medium, Oxoid Preparation:
Suspend 16g in 1 litre of distilled water. Bring to the boil to dissolve completely and dispense into screw-capped 7ml bottles. Fill each bottle to the brim, tighten the cap and sterilise by autoclaving at 121°C for 15 minutes. When sufficiently cool to handle, mix by inversion.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 15-25°C.

Precautions
A small amount of blue colour at the top of the bottle indicates oxidation. If this colour extends down into the medium it should be discarded.
Avoid prolonged heating in open flasks, during the preparation of the medium, because thioglycollate is volatile.
Sodium glycerophosphate may be metabolised by some organisms and thus promote their growth.

Oxoid MacConkey Agar No. 2 is a modification of MacConkey Agar No. 3 with addition of Bile Salts No. 2 for detection of Enterococci.

MacConkey Agar No. 2, Oxoid Composition

MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Detect and enumerate coliform organisms in water, food and dairy products with Thermo Scientific™ Oxoid™ Violet Red Bile Agar (Dehydrated). This lactose containing selective medium has been used for the determination of the coli-aerogenes content of water, milk and other dairy products, dairy equipment, and food products. Due to the addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Agar (Dehydrated), Oxoid Composition

Violet Red Bile Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms e.g. Aeromonas and Yersinia species may give similar reactions.
The selectivity of the medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth.

Oxoid Corn Meal Agar Mycological medium is used for identification of Candida albicans and maintenance of fungal stock cultures.

Corn Meal Agar, Oxoid Composition

Corn Meal Agar, Oxoid Preparation:
Suspend 17g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Glucose supplemented Corn Meal Agar should not be used for chlamydospore production.
Corn Meal Agar with `Tween 80’ (or other wetting agents) will allow Candida stellatoides and Candida tropicalis to produce chlamydospores.
Some Candida strains lose their ability to produce chlamydospores after repeated subculturing.

Isolate and identify Salmonella and Shigella species from clinical or food samples with Thermo Scientific™ Oxoid™ SS Agar (Dehydrated). Differentiation of Salmonella and Shigella is achieved by incorporation of lactose in the medium. Organisms which ferment lactose produce acid which, in the presence of the neutral red indicator, results in the formation of red colonies. Salmonella, Shigella, and other non-lactose-fermenting organisms appear as transparent or straw coloured colonies on SS Agar.

SS Agar, Oxoid Composition

SS Agar, Oxoid Preparation:
Suspend 63g in 1 litre of distilled water. Bring to the boil with frequent agitation and allow to simmer gently to dissolve the agar. DO NOT AUTOCLAVE. Cool to about 50°C, mix and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This medium is highly selective and R-strains of shigellae will not grow on it. It is not recommended for the primary isolation of shigellae.

Oxoid Czapek Dox Agar Modified is used for cultivation of fungi.

Czapek Dox Agar Modified, Oxoid Composition

Czapek Dox Agar Modified, Oxoid Preparation:
Suspend 45.4g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. If required, adjust the reaction to pH 3.5 ± 0.2 by adding 10ml of Lactic Acid 10% (SR0021) per litre after sterilisation.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Czapek Dox Liquid Medium is used for cultivation of fungi.

Czapek Dox Liquid Medium, Oxoid Composition

Czapek Dox Liquid Medium, Oxoid Preparation:
Add 33.4g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.
Store tubes of broth at 15-25°C.

Enrich and isolate halophilic organisms, especially staphylococci, using Thermo Scientific™ Oxoid™ Salt Meat Broth Tablets. Salt Meat Broth Tablets are specially designed for the isolation of staphylococci from grossly contaminated specimens such as feces, particularly during the investigation of staphylococcal food poisoning. The medium is highly sensitive as it can detect even low numbers of staphylococci from samples having large proportions of heterogeneous microbial flora.^1,2

• Quantity: 100 tablets/bottle

Salt Meat Broth Tablets, Oxoid Composition

Salt Meat Broth, Oxoid Preparation:
Add 2 tablets to 10ml of distilled water in an appropriately sized test tube and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Oxoid Tryptone Water is used for detection of indole production.

Tryptone Water, Oxoid Composition

Tryptone Water, Oxoid Preparation:
Dissolve 15g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

• Oxoid Hoyle Medium Base is used for isolation and differentiation of Corynebacterium diphtheriae Types.
• Requires addition of Potassium Tellurite 3.5%, Part No. SR0030J

Hoyle Medium Base, Oxoid Composition

Hoyle Medium, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 55°C and add 50ml of Laked Horse Blood SR0048 and 10ml of 3.5% Potassium Tellurite solution SR0030, mix, and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
It should be noted that not all corynebacteria produce the typical colonies described above – so in all cases it is advisable to use Hoyle medium in conjunction with the additional media and tests mentioned above.

Grow and maintain aerobic and anaerobic organisms with Thermo Scientific™ Oxoid™ Cooked Meat Medium (Dehydrated). The medium contains heart tissue, beef extracts, peptone and glucose for the cultivation of anaerobic and aerobic organisms. It has the ability to initiate bacterial growth from very small inocula and to maintain the viability of cultures over long periods of time1. Mixed cultures of bacteria survive in Cooked Meat Medium without displacing the slower growing organisms.

Cooked Meat Medium, Oxoid Composition

Cooked Meat Medium, Oxoid Preparation:
Suspend 10g in 100ml of distilled water (or 1g amounts in 10ml volumes of water in tubes). Allow to stand for 15 minutes until the meat particles are thoroughly wetted. Sterilise by autoclaving at 121°C for 15 minutes. Do not cool the bottles rapidly because ebullition will expel the meat particles from the containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature, in the dark with tightened caps for up to 6 months.

Precautions
The excellent recovery properties of Cooked Meat Medium mean that mixed cultures commonly result from sample inoculation.
Blackening of the medium will not take place if the pH is acid.
Carbohydrate fermentation may inhibit proteolysis.

Detect thermophilic anaerobic organisms causing sulphide spoilage in food with Thermo Scientific™ Oxoid™ Iron Sulphite Agar (Dehydrated). The medium is a modification of Cameron Sulphite Agar developed by the National Canners Association of America1. Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes.

Iron Sulphite Agar, Oxoid Composition

Iron Sulphite Agar, Oxoid Preparation:
Suspend 23.0g in 1 litre of distilled water and boil to dissolve completely. Sterilise by autoclaving for 15 minutes at 121°C. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Precautions
The blackening reaction is only presumptive evidence of clostridial growth. Confirmation tests must be carried out to identify the organism growing in the medium.

Oxoid Dextrose Tryptone Agar is used for detection and enumeration of thermophilic and mesophilic microorganisms in food.

Dextrose Tryptone Agar, Oxoid Composition

Dextrose Tryptone Agar, Oxoid Preparation:
Suspend 27g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
Incubation at 55°C must be carried out under humid conditions e.g. wrapped dishes or in a high humidity environment.

Oxoid Dextrose Tryptone Broth is used for detection and enumeration of thermophilic and mesophilic microorganisms in food.

Dextrose Tryptone Broth, Oxoid Composition

Dextrose Tryptone Broth, Oxoid Preparation:
Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared broth below 25°C.

Oxoid Urea Broth Base (Christensen Broth Base) is used for the detection of urease producing microorganisms.

Urea Broth Base, Oxoid Composition

Urea Broth Preparation:
Add 0.9 g to 95 ml of distilled water. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 55°C and aseptically introduce 5 ml of sterile 40% Urea Solution SR0020. Mix well and distribute 10 ml amounts into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is preferable that the medium be used on the day of preparation. If not, examine the tubes carefully to ensure sterility.
After overnight incubation other members of the Enterobacteriaceae may show alkaline reactions.

Detect, enumerate and differentiate the members of coliform group with Thermo Scientific™ Oxoid™ Eosin Methylene Blue Agar (Modified) Levine (Dehydrated). This versatile medium, modified by Levine is used for the differentiation of Escherichia coli and Enterobacteria aerogenes and also for rapid identification of Candida albicans. The medium is prepared to the formula specified by the APHA for the detection and differentiation of the coliform group of organisms.

Eosin Methylene Blue Agar (Modified) Levine, Oxoid Composition

Eosin Methylene Blue Agar (Modified) Levine, Oxoid Preparation:
Suspend 37.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidise the methylene blue (i.e. restore its blue colour) and to suspend the precipitate which is an essential part of the medium.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
Further tests are required to confirm the presumptive identity of organisms isolated on this medium. Some strains of Salmonella and Shigella species will not grow in the presence of eosin and methylene blue. Store the medium away from light to prevent photo-oxidation.

This product has been discontinued. The alternative to this product is Peptone Water.

Oxoid Peptone Water Andrade’s is a nutrient base containing Andrade’s indicator to which carbohydrates and other diagnostic reagents may be added for use in fermentation studies.

Peptone Water Andrade’s, Oxoid Composition

Peptone Water Andrade’s, Oxoid Preparation:
Suspend 15 g in 1 litre of distilled water. Mix well to dissolve, distribute into tubes or bottles, containing Durham tubes and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium in the dark at room temperature.