Microbiology

• Oxoid Chromogenic Salmonella Agar Base is a selective chromogenic medium for the presumptive identification of Salmonella spp. from clinical and food samples.
• Also available Salmonella Selective Supplement, Part No. SR0194E and Brilliance™ Salmonella Agar Base, Part No. CM1092B.

Chromogenic Salmonella Agar Base, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 6 weeks at 2-8°C.

• Oxoid Cefixime Rhamnose Sorbitol MacConkey Agar Base (CR-SMAC) is a selective and differential medium containing rhamnose and cefixime for the isolation of E. coli O157.
• Also available Cefixime Supplement, Part No. SR0191E.

CR-SMAC Agar Base, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Brilliance Candida Agar Base is a chromogenic medium for the isolation and differentiation of clinically important Candida spp., including C. albicans.
• Also available Chromogenic Candida Agar Selective Supplement, Part No. SR0231E.

Brilliance Candida Agar Base, Oxoid Composition

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Agar Base must be stored tightly capped in the original container at 10-30°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Isolate Burkholderia cepacia from respiratory secretions of patients with cystic fibrosis with Thermo Scientific™ Oxoid™ Burkholderia Cepacia Agar Base, which is also used for routine testing of non-sterile inorganic salt solutions in hospital environments. The slower growing B. cepacia can be missed on conventional media, such as blood or MacConkey Agar, due to overgrowth caused by other faster growing organisms found in the respiratory tract of CF patients, such as mucoid Klebsiella species,
Pseudomonas aeruginosa and Staphylococcus species.This may lead to the infection being missed or wrongly diagnosed.

Burkholderia Cepacia Agar Base, Oxoid Composition

Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

• Oxoid EC Broth, Reduced Bile Salts is a selective enrichment broth for the growth of Escherichia coli O157 from food and environmental samples.
• Also available Novobiocin Selective Supplement, Part No. SR0181E.

EC Broth (Reduced Bile Salts), Oxoid Composition

EC Broth (Reduced Bile Salts), Oxoid Preparation:
Dissolve 18.3g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool the EC Broth (Reduced Bile Salts) to 50°C and aseptically add the contents of 1 vial of Novobiocin Supplement SR0181, reconsituted as directed. Dispense into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
When stored as directed, the supplement remains stable until the expiry date printed on the label.

Oxoid VRE Agar Base (Vancomycin Resistant Enterococci Agar Base) is used for selective isolation of vancomycin-resistant enterococci and high level aminoglycoside-resistant enterococci (HLARE) from clinical samples.Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Agar Base, Oxoid Composition

VRE Agar, Oxoid Preparation:
Suspend 42.6g of VRE Agar Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121° C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then Mix agar well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.
Warning: Sodium azide is harmful if swallowed, please take all necessary precautions.

This product has been discontinued. Please contact us for more information.

• Oxoid VRE Broth Base (Vancomycin Resistant Enterococci Broth Base) is for selective isolation of vancomycin-resistant enterococci and high Level aminoglycoside-resistant enterococci.
• Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Broth Base, Oxoid Composition

VRE Broth, Oxoid Preparation:
Suspend 37.0g of VRE Broth Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then mix well and distribute into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.

Bolton Broth Base, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.

Carry out microbial limit testing by harmonized methodology of pharmaceutical products in accordance with USP/EP/BP/JP with Thermo Scientific™ Oxoid™ Buffered Sodium Chloride Peptone Solution (Dehydrated). The combination of phosphate buffer, sodium chloride and peptone increases the viability of sensitive microorganisms by providing an isotonic environment. This is of particular importance when trying to recover cells that may be stressed or sensitive to osmotic pressure.

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Composition

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Preparation:
Suspend 14.63g of Sodium Chloride Peptone Broth (Buffered) in 1 litre of water (purified as required). If required, add 1-10ml Tween® 20 or 80 per litre of medium. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared media at room temperature.

• Oxoid Sorbitol MacConkey Agar w/BCIG is a selective medium containing the chromogenic substrate BCIG.
• Can be used with C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar w/BCIG, Oxoid Composition

Sorbitol MacConkey Agar w/BCIG, Oxoid Preparation:
Suspend 51.6g of SMAC with BCIG in 1 litre of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Lauryl Tryptose Broth w/MUG is used for the presumptive identification of E. coli.

Lauryl Tryptose Broth with MUG, Oxoid Composition

Lauryl Tryptose Broth, Oxoid Preparation:
Suspend 35.65g of Lauryl Tryptose Broth with MUG in 1 litre of distilled water. Warm to 40°C. Mix well to dissolve and distribute into final containers holding inverted Durham tubes. (Please be aware of the special glassware requirements when using ultra-violet light.). Sterilise by autoclaving at 121° C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Oxoid EC Broth with MUG is used for the isolation and presumptive identification of E. coli.

EC Broth with MUG, Oxoid Composition

EC Broth with MUG, Oxoid Preparation:
Suspend 37g of EC Broth with MUG in 1 litre of distilled water. Mix well to dissolve and distribute into final containers containing inverted Durham tubes. Sterilise by autoclaving at 121° C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Ensure that the glass tubes used for the preparation of the medium do not auto fluoresce and take the relevant safety precautions when using a UV lamp.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give a positive result in this test.
Other enteric organisms e.g. some Shigella species are also glucuronidase positive.

Oxoid Violet Red Bile Agar w/MUG is used for the presumptive identification of E. coli.

Violet Red Bile Agar with MUG, Oxoid Composition

Violet Red Bile Agar with MUG, Oxoid Preparation:
Suspend 38.6g of VRBA with MUG in 1 litre of distilled water. Bring to the boil to dissolve completely. No further sterilisation is necessary or desirable. Mix well before pouring into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

• Oxoid Anaerobe Basal Agar is used for growth of fastidious and other anaerobes.
• Also available N-S Anaerobe Selective Supplement, Part No. SR0107B, for use for nonsporforming anaerobes and G-N Anaerobe Selective Supplement, Part No. SR0108B, for use for gram-negative anaerobes.

Anaerobe Basal Agar, Oxoid Composition

Anaerobe Basal Agar, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium for upto 3 weeks at 2-8°C.

Isolate and enumerate coliforms in food, animal feed and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), while adhering to ISO 4832:2006 and ISO/TS 11133-2:2003. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBL Agar (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBL Agar (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBL Agar (ISO) plates can be stored for up to 2 weeks when made from CM0968 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBL Agar (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBL Agar (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

• Oxoid Arcobacter Broth Base is used for the selective growth of Arcobacter spp.
• Also available CAT Supplement, Part No. SR0174E and CCDA Selective Supplement, Part No. SR0155E and SR0155H.

Arcobacter Broth Base, Oxoid Composition

Arcobacter Broth Base, Oxoid Preparation:

Dissolve 12g of Arcobacter Broth in 500ml of distilled water. Sterilise at 121°C for 15 minutes and allow to cool to below 50°C

For use with CAT supplement (SR0174)

Add one vial per 500ml medium of CAT Selective Supplement reconstituted as directed. Dispense into sterile containers.

For use with CCDA supplement (SR0155)

Add one vial per 500ml medium of CCDA Selective Supplement reconstituted as directed. Dispense into sterile containers.

Inoculated plates should be incubated aerobically, at 30°C, for 24 hours.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

• The Oxoid Baird-Parker RPF Agar Base is a selective medium for the isolation and enumeration of coagulase positive staphylococci from food.
• For use with RPF supplement, Part No. SR0122A
• Do not use Egg Yolk Tellurite Emulsion.

Baird-Parker Agar Base (RPF), Oxoid Composition

Baird-Parker Agar Base (RPF), Oxoid Preparation:
Suspend 6.3g of Baird-Parker Agar Base (RPF) in 90ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121ºC for 15 minutes. Cool to 48ºC and add 1 vial of SR0122A, reconstituted as directed. Mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and RPF Supplement below 0°C. Use before the expiry date on the label.
Prepared plates of medium are best used freshly prepared.

Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

Cultivate anaerobic microorganisms, particularly Bacteroides spp. and other fastidious anaerobes with Thermo Scientific™ Oxoid™ Anaerobe Basal Agar (Dehydrated). Anaerobe Basal Broth is formulated from a range of nutrients which have been selected to optimize the recovery and growth of the majority of anaerobic organisms of clinical importance.

Anaerobe Basal Broth, Oxoid Composition

Anaerobe Basal Broth, Oxoid Preparation:
Suspend 35.4g of Anaerobe Basal Broth in 1 litre of distilled water. Bring to the boil to dissolve. Distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Anaerobe Basal Broth should be stored tightly capped in the original container at 10-30°C. When stored as directed, the medium will remain stable until the expiry date printed on the label.

Brilliance E. coli/Coliform Agar is a chromogenic medium for differentiation of Escherichia coli and other coliforms from food and environmental samples.

Brilliance E. coli/Coliform Agar, Oxoid Composition

Brilliance E. coli/Coliform Agar, Oxoid Preparation:
Suspend 55.8g of Brilliance E.coli/coliform agar in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour and into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C, and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Refer to the material safety data sheet before handling this product.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give purple colonies on this medium.

Detect and enumerate Escherichia coli in food and animal feed samples with Thermo Scientific™ Oxoid™ Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated). The medium meets the formulation and performance criterion laid down in ISO 16649 and ISO 11133:2014.

TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Composition

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Preparation:
Suspend 36.6g of TBX Medium in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour the medium into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

• Oxoid Campylobacter Karmaili Agar Base is a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.
• Also available Campylobacter Selective Supplement (Karmaili), Part No. SR0167E and Modified Karma-i-li Selective Supplement, Part No. SR0205E.

Campylobacter Karmaili Agar Base, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.

Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.

MYP Agar, Oxoid Composition

MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Yeast and Mold Agar is used for the isolation and maintenance of yeast and molds.

Yeast and Mold Agar, Oxoid Composition

Yeast and Mold Agar, Oxoid Preparation:
Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
For in vitro diagnostic use.
Do not use beyond the stated expiry date or if the product is caked, discoloured or shows any sign of deterioration.