PCR, Polymerase & Amplification Technologies

Bst 2.0 DNA Polymerase displays improved amplification speed, yield and salt tolerance

• Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
• Improved amplification reaction properties compared to Bst DNA Polymerase
• Fast polymerization
• Flexible reaction conditions including a higher salt tolerance and thermostability, as compared to wild-type Bst DNA Polymerase, Large Fragment (NEB #M0275)

Optimized for Loop-Mediated Isothermal Amplification (LAMP)

• Consistent amplification performance with the convenience of room temperature setup
• WarmStart^® technology eliminates off-target amplifilcation and offers increased reaction efficiency
• Optimal reaction performance from 60-72 °C

Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.

The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)

• Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
• Suitable for applications requiring thermophilic strand displacement
• Supplied with ThermoPol^® Reaction Buffer for high product yields in demanding conditions

Enhance your PCR Reaction with our ultrapure dNTPs

• Compatible with all NEB DNA polymerases in a variety of applications
• ≥ 99% pure as determined by HPLC analysis
• Exceptional stability

The industry standard with the hot start peformance

• Ideal for routine PCR applications
• Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
• 2X master mix format for easy reaction setup – just add template DNA and primers

The industry standard with hot start performance

• Ideal for routine PCR applications and molecular diagnostics
• High specificity and sensitivity
• Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup

Exceptional performance with long amplicons

• Optimized enzyme blend for amplification up to 30 kb
• 2X higher fidelity than Taq
• Master mix is a 2X concentrated solution containing everything needed for robust amplification of complex or simple templates

Make a simpler choice

• One product per application simplifies selection
• Convenient master mix formats and user-friendly protocols simplify reaction setup
• Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

• All Luna^® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
• Products perform consistently across a wide variety of sample sources
• A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your RT-qPCR with Luna WarmStart^® Reverse Transcriptase

• Novel, thermostable reverse transcriptase (RT) improves performance
• WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness

Make a simpler choice

• One product per application simplifies selection
• Convenient master mix formats and user-friendly protocols simplify reaction setup
• Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

• All Luna^® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
• Products perform consistently across a wide variety of sample sources
• A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your RT-qPCR with Luna WarmStart^® Reverse Transcriptase

• Novel, thermostable reverse transcriptase (RT) improves performance
• WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness

Rapid, sensitive and precise probe-based qPCR detection and quantitation of target DNA and cDNA sequences.

Make a simpler choice

• One product per application simplifies selection
• Convenient master mix formats and user-friendly protocols simplify reaction setup
• Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

• All Luna^® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
• Products perform consistently across a wide variety of sample sources
• A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences.

Make a simpler choice

• One product per application simplifies selection
• Convenient master mix formats and user-friendly protocols simplify reaction setup
• Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

• All Luna^® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
• Products perform consistently across a wide variety of sample sources
• A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

The LunaScript^® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

Make a simpler choice

• Single-tube SuperMix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna^® Reverse Transcriptase
• Non-interfering, visible tracking dye helps to eliminate pipetting errors
• Combine with Luna qPCR master mixes for robust RT-qPCR results

Experience best-in-class performance

• Less than 15 minute first-strand cDNA synthesis protocol
• All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
• A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Illuminate your two-step RT-qPCR

• Novel, thermostable Luna Reverse Transcriptase improves performance
• Consistent linearity, sensitivity, and capacity for reliable RNA quantification

M-MuLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.

• Isolated from a recombinant source
• Supplied with 10X Reaction Buffer
• Tested for the absence of DNases and RNases

OneTaq^® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

• Obtain high yields across a wide range of AT / GC content
• 2X higher fidelity than Taq
• Master mix is a 2X concentrated solution containing everything needed for robust amplification

The ONE polymerase for your endpoint PCR needs. OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent^® DNA polymerases for use with routine and difficult PCR experiments.

• 2X higher fidelity than Taq
• Ideal for routine, AT- or GC-rich templates
• Hot start and master mix versions available