PCR, Polymerase & Amplification Technologies

OneTaq^® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

• Obtain high yields across a wide range of AT / GC content
• 2X higher fidelity than Taq
• Hot Start feature enables room temperature reaction setup
• Master mix is a 2X concentrated solution containing everything needed for robust amplification

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

• Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step
• Robust yield with minimal optimization
• Ideal for routine, AT- or GC-rich templates
• Room temperature reaction setup

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

• Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
• Obtain high yields across a wide range of AT / GC content
• Offered with inert tracking dyes for easy and direct loading of PCR products onto gels

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent^® DNA polymerases for use with routine and difficult PCR experiments.

• Obtain high yields across a wide range of AT- and GC- content
• 2X higher fidelity than Taq
• Master mix format contains inert tracking dye for easy and direct loading of PCR products onto gels

Limited-time promotion! Buy M0509Sx5 (5 tubes of OneTaq Quick-Load DNA Polymerase, 100 units) for RM500 only! Before 13th November 2020!

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

• Includes green Quick-Load reaction buffer for easy and direct loading of PCR products onto gels
• 2X higher fidelity than Taq
• Compatible with standard Taq protocols

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific^® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

• 50X higher fidelity than Taq
• Robust reactions – maximal success with minimal optimization
• Offered with multiple buffers for customized reaction setup for different DNA templates

Ideal for high speed and high-performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific^®Phusion^® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

• 50X higher fidelity than Taq
• Robust Reactions – maximal success with minimal optimization
• Kit includes everything needed for a PCR reaction, including controls

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific^® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

• 50X higher fidelity than Taq
• Robust reactions – maximal success with minimal optimization
• Master mix format with GC buffer ideal for long, difficult, or GC-rich DNA templates

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific^® Phusion^® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

• 50X higher fidelity compared to Taq
• Robust reactions – maximal success with minimal optimization
• Master mix format with HF buffer ideal for a wide range of AT/GC content

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific^® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

• 50X higher fidelity than Taq
• Hot start formulation reduces non-specific amplification
• Master mix format for ease of use and optimized performance

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

• 50X higher fidelity than Taq
• Robust reactions with minimal optimization
•  Hot start formulation enables room temperature reaction setup and reduces non-specific amplification
• High yields with shorter extension times

The ProtoScript^® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA.

• Recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability (active up to 48°C)
• Generates cDNA up to 10 kb
• Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free water

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot start and master mix formats available

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot Start and Master Mix formats available

Fidelity at its finest

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot start and master mix formats available

Fidelity at its finest

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot start and master mix formats available

Q5^® High-Fidelity DNA Polymerase, NEB sets a new standard for both fidelity and robust performance. With the highest-fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Features of Q5^® High-Fidelity DNA Polymerase, NEB:

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot start and master mix formats available

Q5U^® Hot Start High-Fidelity DNA Polymerase is a modified version of Q5^® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U^® contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

• Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
• Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
• Generate higher assembly efficiency and improve accuracy in USER® cloning
• Enable room temperature setup with aptamer-based hot start formulation
• Utilize optimized protocols for recommended applications

The industry standard

• Ideal for routine PCR applications
• Exceptional value in terms of cost per unit
• Mg-free buffer offers flexibility to adjust magnesium concentrations for use with microarray or DHPLC applications