Paternity Testing – Electrophoresis Education kit
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Cleaver Scientific
In this experiment, DNA from a mother, child and 2 possible fathers is seperated by electrophoresis to determine the paternity of the child. The human DNA is simulated with a series of unqiue fragments that form a profile for each sample, allowing students to identify the father based on the mother and childs seperation profiles.
This kit includes DNA, Agarose, electrophoresis buffer and DNA stain for 8 student groups.
Key Features
- Perfect for 1 hour lessons
- Introduce students to DNA and genetics
- Works perfectly with Cleaver Scientific equipment
Cystic Fibrosis – Electrophoresis Education kit
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Cleaver Scientific
Cystic Fibrosis is one of the most common genetic conditions in the UK. In this educational kit, students will learn about the basis of the disease, and run an agarose gel to seperate DNA samples from healthy patients, carriers of the disease and CF patients.
Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels).
Key Features
- Whole experiment takes less that 1 hour
- Great for introducing genetic diseases and genetic screening to students
- Works perfectly with Cleaver Scientific electrophoresis equipment
DNA Fingerprinting – Electrophoresis Education kit
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Cleaver Scientific
Our DNA fingerprinting kit is the perfect tool to introduce students to the world of electrophoresis and forensic science. DNA fingerprinting is a laboratory technique used to establish a link between biological evidence and a suspect in a criminal investigation. A DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA profiles are a match, then the evidence came from that suspect. Conversely, if the two DNA profiles do not match, then the evidence cannot have come from the suspect.
Each kit contains DNA, agarose gel, electrophoresis buffer and DNA stain enough for 8 groups of students to cast and run the experiment and visualise the DNA separation. The gel takes approximately 5 minutes to load, 30 minutes to run and 15 minutes to stain, meaning the whole experiment can be completed in a one hour lesson, with convenient incubation periods for scientific discussion and teaching. Students will solve a crime using an unknown DNA sample from the crime scene and a series of suspects!
Key Features
- Perfect for 1 hour lessons
- Introduce students to DNA and forensic science
- Works perfectly with Cleaver Scientific equipment
Q5U® Hot Start High-Fidelity DNA Polymerase, NEB
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New England Biolabs
Q5U® Hot Start High-Fidelity DNA Polymerase is a modified version of Q5® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U® contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
- Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
- Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
- Generate higher assembly efficiency and improve accuracy in USER® cloning
- Enable room temperature setup with aptamer-based hot start formulation
- Utilize optimized protocols for recommended applications
Nitrile Examination Glove (Powder Free)
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Lattice
Powder-free nitrile examination glove is the most common disposable gloves that use in different applications.
Features
- Protect from cross-contamination and dangerous substances
- Smooth donning thanks to single chlorination process even with a wet hand
- Finger textured enhances wet & dry grip
- Ambidextrous
- Custom design enhances comfort and fit
- Beaded cuff makes donning easy
- An alternative solution for individuals who are allergic to natural rubber latex
| Type | : | Powder Free |
| Material | : | High-quality synthetic rubber latex (Nitrile) |
| Design & Features | : | Ambidextrous, finger textured, beaded cuff, cornflower blue |
| Shelf-life | : | 5 years from the date of manufacturing |
Quality Standards
- Conforms to ASTM D3578 & EN455 standards
- Manufactured under ISO13485:2003 quality management system
Latex Examination Glove (Powder Free)
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Lattice
Powder-free latex examination glove is the most common disposable gloves that use in different application.
Features
- Protect from cross-contamination and dangerous substances
- Smooth donning thanks to single chlorination process even with a wet hand
- Soft and flexible to provide comfort
- Ambidextrous
- Fully textured to improve grip even in wet condition
- Beaded cuff makes donning easy
| Type | : | Powder Free |
| Material | : | High-quality natural rubber latex |
| Design & Features | : | Ambidextrous, textured, beaded cuff, off white to yellow |
| Shelf-life | : | 5 years from the date of manufacturing |
Quality Standards
- Conforms to ASTM D3578 & EN455 standards
- Manufactured under ISO13485:2003 quality management system
Monarch RNA Cleanup Kit (500 µg), NEB
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New England Biolabs
The Monarch RNA Cleanup Kit (500 µg), NEB enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.
- Ideal for purification of synthesized RNA following high-yield in vitro transcription reactions (alternative to MEGAclear™)
- Optimized for the cleanup of RNA after enzymatic treatments including DNase I, Proteinase K, labeling and capping
- Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
- Elute in ≥ 50 µl for concentrated RNA 70-100% RNA recovery
- Unique column design prevents buffer carryover and elution of silica particulates
- Simplified workflow with a single wash buffer
- Columns and buffers are available separately
- Purified RNA is ready for use in a wide variety of downstream applications, including microinjection and transfection.
Monarch® RNA Cleanup Kit (50µg), NEB
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New England Biolabs
The Monarch RNA Cleanup Kit (50 µg), NEB enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.
- Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labelling, capping or in vitro transcription (IVT)
- Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
- Elute in ≥ 20 µl for concentrated RNA
- 70-100% RNA recovery
- Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
- Simplified workflow with a single wash buffer
- Unique column design prevents buffer carryover and elution of silica particulates
- Columns and buffers are available separately
- Purified RNA is ready for use in a wide variety of downstream applications, including transfection
Eppendorf Research® plus Multi-channel Micropipettes
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Eppendorf
Description
The Research plus is the pipette family with the lowest weight and lowest operation forces in the Eppendorf product families. Lower operation forces can only be reached with our Eppendorf electronic pipettes. Enjoy excellent flexibility and choose among single-channel pipettes in fixed or variable volume as well as multi-channel pipette options with 8, 12, 16 and 24 channels.
Key Features:
- Air-displacement pipette for accurate pipetting of aqueous solutions.
- Feel the difference in weight and pipetting forces: the ultra-light mechanical pipette is designed according to the strict criteria of the Eppendorf PhysioCare Concept and limits the strain on your hand and arm
- Spring-loaded tip cone (available for all pipettes up to 1 mL) for minimal tip attachment forces helps to reduce stress
- Low tip ejection force of Eppendorf Research plus can be as low as 3.6 N
- Adjust your pipette in seconds for better accuracy when pipetting various difficult liquids like ethanol or even when pipetting at high altitudes. Return to factory adjustment without calibration
- Autoclave the entire pipette or only the lower part according to your needs to ensure decontamination
L10 Jumbo Roll/Industrial Roll Wipers, WYPALL
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WYPALL
Jumbo Roll Wipers, also known as industrial roll wipers, are widely used in the laboratory for cleaning purpose. It is extremely easy to use when use with the stands.
OmniPAGE Mini Vertical Protein Electrophoresis System
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Cleaver Scientific
Cleaver Scientific Mini Vertical systems are predominantly used for protein electrophoresis. They include the OmniPAGE CVS10TETRAD systems equipped with enough combs and glass plates to run 4 gels, and the standard OmniPAGE CVS10 systems, which accommodate up to 2 handcast or commercial precast gels.
| Specifications |
Descriptions |
| Gel Dimensions (w x l) |
8 x 8.5cm |
| Unit Dimensions (w x l x h) |
19 x 13 x 15cm |
| Max Sample/ Gel Capacity |
80 samples/ 1-4 gels |
| Base Buffer Volume |
250ml |
| Typical Running Conditions |
90V, 45-60 mins |
| Typical Running Conditions |
1-2 hours at 90-225v |
| Plate Dimensions |
10 x 10 x 0.2cm |
Eppendorf Research® plus G Micropipette
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Eppendorf
Description
Eppendorf Research® plus Micropipette is the lowest weight and lowest operation forces in the Eppendorf pipette families. Enjoy excellent flexibility and choose among single-channel Eppendorf pipettes in variable volume, ranging from 0.1µL to 10mL. Eppendorf Research® plus Micropipette has a large display volume of 4 units, which help you to have a more accurate pipetting volume.
Key Features of Eppendorf Micropipette:
- Air-displacement pipette for accurate pipetting of aqueous solutions.
- Feel the difference in weight and pipetting forces
- Spring-loaded tip cone (available for all pipettes up to 1 mL) for minimal tip attachment forces helps to reduce stress
- Low tip ejection force of Eppendorf Research® plus micropipette plus can be as low as 3.6 N
- Adjust your Eppendorf pipette in seconds for better accuracy when pipetting various difficult liquids like ethanol or even when pipetting at high altitudes. Return to factory adjustment without calibration
- Autoclave the entire Eppendorf pipette or only the lower part according to your needs to ensure decontamination
TE Buffer
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
‘TE is deriving from ‘Tris’, a common pH buffer and ‘EDTA’, a cation chelators like Mg2+. TE buffer is normally used in molecular biology to solubilize DNA, RNA or cDNA while protecting it from degradation, or used in the cell culture. Here, we provide TE buffer in 10x or 100x with pH ranging from 7.4 to 8.
TAE Buffer Solution
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
TAE buffer 50x solution: TAE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This 25x and 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.
1X and 10X TBE buffer (Tris-Borate-EDTA), Biobasic
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
TBE buffer (Tris-Borate-EDTA, Biobasic) is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments; whereas, TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. BIO BASIC INC. offers TBE in a number of different formats. 10X TBE liquid concentrate can be used to easily prepare a 1X TBE working solution by diluting with distilled, deionized water. 10X TBE Powder allows for the stable storage of the pre-mixed powder form that can be dissolved in distilled, deionized water to yield 1 liter each of 10X TBE buffer solution.
How to prepare a 10X TBE Buffer?
10X TBE buffer preparation is easy. Simply mix 10X TBE powder (SKU: A00269-500ml/A0026-4L) with an appropriate volume of distilled/deionized water. The 10X TBE buffer is ready to use now.
RNase and DNAase Away
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
RNase and DNAase AwayTM: DB0339 efficiently remove surface-contaminant from glasswares and plasticwares without having a residual effect on subsequent DNA & RNA samples . The product provides more effective at degradading DNA than autoclave.
TheraPEAK™ MSCGM™ CD Serum-free Mesenchymal Stem Cell Growth Medium BulletKit™
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Lonza
** This product has been discontinued.
Description
Stem cells possess the unique ability to proliferate as an undifferentiated cell type, then, when properly stimulated, differentiate into multiple lineages of cells. Lonza’s TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium is a chemically defined, serum-free, xeno-free media designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state. The medium has been specifically designed and tested to ensure that the medium neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media). Serum-free media, such as TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium, are intended to support cell growth without serum supplementation. This medium is chemically defined indicating that it contain only constituents of known molecular structure. Lonza’s TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium is of primary interest to those seeking maximal control over research and manufacturing variables. TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium’s formulation is complete and contains human albumin, recombinant human insulin, pasteurized human transferrin, HEPES, and L-glutamine. TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium does not contain phenol red or antibiotics. The basal medium (MSCBM-CD Mesenchymal Basal Medium, Chemically defined; catalog no.00190620) and the necessary supplements (MSCGM-CD™ SingleQuotsTMKit ; catalog no. 00192125) are included in this BulletKit.
X-VIVO™ 10 Serum-free Hematopoietic Cell Medium, xenofree
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Lonza
Description
The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVO™ 10 Media is available as a 1X liquid in two convenient formulations.
Key Applications:
-Proliferation of peripheral blood lymphocytes
-Proliferation of tumor infiltrating lymphocytes
-Cryopreservation of human tissue
-Cultivation of human monocytes and macrophages
-Cultivation of stem cells
-Cultivation of dendritic cells
X-VIVO™ 15, Serum free hematopoietic cell medium, xenofree
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Merck
Description
X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 Media and have been optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. X-VIVO™ 15 Media support the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells. In addition, X-VIVO™ 15 Media provide a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.
Applications:
-Proliferation of peripheral blood lymphocytes
-Proliferation of tumor infiltrating lymphocytes
-Cryopreservation of human tissue
-Cultivation of human monocytes and macrophages
-Cultivation of stem cells
-Cultivation of dendritic cells
-Cultiviation of CAR-T cells
X-VIVO™ 20 Serum-free Hematopoietic Cell Medium
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Lonza
Description
X-VIVO™ 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocytedepleted peripheral blood lymphocytes (PBL) at high density. Initial cell densities between 2.0 – 3.0 × 107 cells/ ml can be used to successfully generate LAK cells. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).
All BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures.
Alcohol 70%
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Chemiz
Description
Synonyms: Ethyl-alcohol
Formula: C2H6O
Chemically pure grade ethanol (denatured) is used in general chemistry application. 70% denatured ethanol is used in most histology and cytology bioassays. It is also used as disinfectant in many lab applications.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate
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Merck
Description
Histone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 Conjugate
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Merck
Description
As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (mouse monoclonal)
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Merck
Description
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ‘beads on a string’ structure
