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• Oxoid K-F Streptococcus Agar is a selective medium for the isolation and enumeration of Group D Streptococci.
• Also available TTC Solution, Part No. SR0148A.

K-F Streptococcus Agar Base, Oxoid Composition

K-F Streptococcus Agar, Oxoid Preparation:
Suspend 38.2g in 500ml of distilled water. Bring to the boil with frequent agitation. Boil for 5 minutes. Cool to 50°C and add aseptically 1 vial (5ml) of 1% 2,3,5-Triphenyltetrazolium chloride (SR0229K). Pour into sterile Petri dishes when using the membrane filtration method or hold at 45°C when using the pour-plate method.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
Observe the HAZARD precautions regarding sodium azide when disposing of the medium. The pH of the medium should not fall below 7.0 as it may become inhibitory towards enterococci. KF Streptococcus Agar is not specific for the presumptive identification of Group D streptococci. Further tests must be made to confirm the identity of the organisms isolated.

• Oxoid Kanamycin Aesculin Azide Agar Base is a selective medium for the isolation of Enterococci in foodstuffs.
• Also available Kanamycin Sulphate Selective Supplement, Part No. SR0092E.

Kanamycin Aesculin Azide Agar Base, Oxoid Composition

Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Suspend 21.3g in 500ml of distilled water. Add one vial of Kanamycin Supplement (SR0092) reconstituted as directed. Bring to the boil. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.

Precautions
Observe the hazard precautions regarding sodium azide when disposing of the medium.
There is no universal medium which will isolate all strains of enterococci.
Unless a presumptive count is acceptable all isolates should have their identity confirmed with further tests.

Oxoid Kanamycin Sulphate Supplement (10mg/vial) is added to Kanamycin Esculin Azide Agar Base, Part No. CM0591B.

Kanamycin Sulphate Supplement, Oxoid Composition

Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Add 2ml of sterile distilled water to one vial and mix gently to dissolve completely. Add the contents to 500ml of Kanamycin Aesculin Azide Agar Base.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.

• Oxoid Campylobacter Karmaili Agar Base is a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.
• Also available Campylobacter Selective Supplement (Karmaili), Part No. SR0167E and Modified Karma-i-li Selective Supplement, Part No. SR0205E.

Campylobacter Karmaili Agar Base, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Oxoid Kligler Iron Agar is used for differential identificaton of Enterobacteriaceae.

Kligler Iron Agar, Oxoid Composition

Kligler Iron Agar, Oxoid Preparation:
Suspend 55g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set as slopes with 1 inch butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is essential that Kligler Iron Agar is examined and reported at 18-24 hours. Early or late readings will give false results.
KIA will grow both oxidative and fermentative organisms. Confusion will result if care is not taken to distinguish between the two groups.
Always use a straight wire to inoculate the butt, to avoid splitting the agar with a loop.
Pure cultures are essential to avoid misinterpretation.
Do not use screw-capped tubes or bottles for KIA medium. It is essential that air is freely available to growth on the slant.

Oxoid Lab-Lemco Broth is a nutrient medium equivalent to USA nutrient extract formulations.

Lab-Lemco Broth, Oxoid Composition

Lab-Lemco Broth, Oxoid Preparation:
Dissolve 8g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.
`Lab-Lemco’ Broth is slightly more alkaline than the medium used in the APHA publications (pH 6.8).

Thermo Scientific™ Oxoid Lab-Lemco Powder is a Beef Extract made from specially selected raw materials.

• May be used in fermentation processes
• Industrial Reference: NMKL

Oxoid Lactalbumin Hydrolysate is a pancreatic digest of lactalbumin which contains high levels of essential amino acids.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Oxoid Lactose Bacteriological is a sugar suitable for inclusion in bacteriological culture media.