Instant Sticky-end Ligase Master Mix
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New England Biolabs
Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.
- No incubation time necessary to achieve ligation
- Efficiencies sufficient for successful sub-cloning of sticky-end substrates
- No thawing of the master mix is required as liquid state is maintained when stored at -20°C
- Simplified reaction setup with optimized ratio of enzyme and buffer components
- Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Ipm10 Imipenem
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Thermo ScientificTM OxoidTM
Iron (III) Chloride 6H2O, AR, 500g
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Chemiz
Description
Synonyms: Ferric chloride hexahydrate
Formula: FeCl3 · 6H2O
Iron (III) chloride hexahydrate is used to detect the presence of phenols and phenolic derivatives. It is able to detect gamma-hydroxybutyric acids and salicylic acids in Trinder spot test.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Iron (III) nitrate 9H2O, CP, 500g
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Chemiz
Description
Synonyms: Ferric nitrate nonahydrate
Formula: Fe(NO₃)₃·9H₂O
Iron (III) nitrate nonahydrate is a water soluble crystalline iron source for uses compatible with nitrates and low pH. It is also used in cell culturing.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Iron puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)-Sigma-Aldrich
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Sigma
Description
Cas Number :7439-89-6
Molecular mass :55.85 g/mol
Chemical formula : Fe
Synonyms : Fe
Iron (carbonyl) powder has been used:
In the preparation of copper on iron (Cu/Fe) bimetallic catalytic system to synthesize β-aminoenones and 3,5-disubstituted pyrazoles.[1]
As the main component of magnetorheological fluids.[2][3]
To produce Fe-B-C composites from boron carbide using plasma sintering.[4]
Iron Standard Solution, Traceable to SRM from NIST (Merck)
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Merck
CAS no.: 10421-48-4, 7697-37-2
Traceability: Traceable to SRM from NIST Fe(NO₃)₃ in HNO₃ 0.5 mol/l 1000 mg/l Fe Certipur®
Synonym: –
Quality level: MQ300
Product specification and SDS for Iron Standard Solution.
Iron Sulphite Agar 500g
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Thermo ScientificTM OxoidTM
Detect thermophilic anaerobic organisms causing sulphide spoilage in food with Thermo Scientific™ Oxoid™ Iron Sulphite Agar (Dehydrated). The medium is a modification of Cameron Sulphite Agar developed by the National Canners Association of America1. Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes.
Iron Sulphite Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 10.0 |
| Sodium sulphite | 0.5 |
| Iron (III) citrate | 0.5 |
| Agar | 12.0 |
| pH 7.1 ± 0.2 @ 25°C |
Iron Sulphite Agar, Oxoid Preparation:
Suspend 23.0g in 1 litre of distilled water and boil to dissolve completely. Sterilise by autoclaving for 15 minutes at 121°C. Mix well before pouring.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.
Precautions
The blackening reaction is only presumptive evidence of clostridial growth. Confirmation tests must be carried out to identify the organism growing in the medium.
Iso-Sensitest Agar 500g
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Iso-Sensitest Agar is a semi-defined medium designed for antimicrobial susceptibility testing.
Iso-Sensitest Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Hydrolysed casein | 11.0 |
| Peptones | 3.0 |
| Glucose | 2.0 |
| Sodium chloride | 3.0 |
| Soluble starch | 1.0 |
| Disodium hydrogen phosphate | 2.0 |
| Sodium acetate | 1.0 |
| Magnesium glycerophosphate | 0.2 |
| Calcium gluconate | 0.1 |
| Cobaltous sulphate | 0.001 |
| Cupric sulphate | 0.001 |
| Zinc sulphate | 0.001 |
| Ferrous sulphate | 0.001 |
| Manganous chloride | 0.002 |
| Menadione | 0.001 |
| Cyanocobalamin | 0.001 |
| L-Cysteine hydrochloride | 0.02 |
| L-Tryptophan | 0.02 |
| Pyridoxine | 0.003 |
| Pantothenate | 0.003 |
| Nicotinamide | 0.003 |
| Biotin | 0.0003 |
| Thiamine | 0.00004 |
| Adenine | 0.01 |
| Guanine | 0.01 |
| Xanthine | 0.01 |
| Uracil | 0.01 |
| Agar | 8.0 |
| pH 7.4 ± 0.2 @ 25°C |
Iso-Sensitest Agar, Oxoid Preparation:
Suspend 31.4g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C and use as freshly as possible.
Iso-Sensitest Broth 500g
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Thermo ScientificTM OxoidTM
Perform effortless testing using Thermo Scientific™ Oxoid™ Iso-Sensitest Broth (Dehydrated), a semi-defined medium specifically designed for antimicrobial susceptibility testing (AST). Iso-Sensitest Broth is carefully constructed to give reproducible results. It allows the growth of the majority of microorganisms without the need of further supplementation.
Iso-Sensitest Broth, Oxoid Composition
| Typical Formula* | gm/litre |
| Hydrolysed casein | 11.0 |
| Peptones | 3.0 |
| Glucose | 2.0 |
| Sodium chloride | 3.0 |
| Soluble starch | 1.0 |
| Disodium hydrogen phosphate | 2.0 |
| Sodium acetate | 1.0 |
| Magnesium glycerophosphate | 0.2 |
| Calcium gluconate | 0.1 |
| Cobaltous sulphate | 0.001 |
| Cupric sulphate | 0.001 |
| Zinc sulphate | 0.001 |
| Ferrous sulphate | 0.001 |
| Manganous chloride | 0.002 |
| Menadione | 0.001 |
| Cyanocobalamin | 0.001 |
| L-Cysteine hydrochloride | 0.02 |
| L-Tryptophan | 0.02 |
| Pyridoxine | 0.003 |
| Pantothenate | 0.003 |
| Nicotinamide | 0.003 |
| Biotin | 0.0003 |
| Thiamine | 0.00004 |
| Adenine | 0.01 |
| Guanine | 0.01 |
| Xanthine | 0.01 |
| Uracil | 0.01 |
| pH 7.4 ± 0.2 @ 25°C |
Iso-Sensitest Broth, Oxoid Preparation:
Add 23.4g to 1 litre of distilled water. Mix well and heat to dissolve. Distribute into tubes or bottles. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Isooctane for analysis EMSURE® ACS,Reag. Ph Eur (Merck)
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Merck
CAS no.: 540-84-1
Grade: For analysis EMSURE® ACS,Reag. Ph Eur
Chemical Formula: CH₃C(CH₃)₂CH₂CH(CH₃)CH₃
Synonym: 2,2,4-Trimethylpentane, Isobutyltrimethylmethane, iso-Octane
Quality level: MQ300
Product specification and SDS for Isooctane for analysis EMSURE®.
Isooctane for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 540-84-1
Chemical Formula: CH₃C(CH₃)₂CH₂CH(CH₃)CH₃
Molar Mass: 114.23 g/mol
Synonyms: 2,2,4-Trimethylpentane, Isobutyltrimethylmethane, iso-Octane
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter
Isopropyl alcohol (2-propanol) AR, 2.5L
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Chemiz
Description
Synonyms: 2-propanol
Formula: (CH3)2CHOH
Isopropyl alcohol is a colourless, flammable and has strong odour compound. It is commonly used as analytical solvent reagent.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
K-F Streptococcus Agar 500g
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Thermo ScientificTM OxoidTM
- Oxoid K-F Streptococcus Agar is a selective medium for the isolation and enumeration of Group D Streptococci.
- Also available TTC Solution, Part No. SR0148A.
K-F Streptococcus Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Proteose peptone | 10.0 |
| Yeast extract | 10.0 |
| Sodium chloride | 5.0 |
| Sodium glycerophosphate | 10.0 |
| Maltose | 20.0 |
| Lactose | 1.0 |
| Sodium azide | 0.4 |
| Bromo-cresol purple | 0.015 |
| Agar | 20.0 |
| pH 7.2 ± 0.2 @ 25°C |
K-F Streptococcus Agar, Oxoid Preparation:
Suspend 38.2g in 500ml of distilled water. Bring to the boil with frequent agitation. Boil for 5 minutes. Cool to 50°C and add aseptically 1 vial (5ml) of 1% 2,3,5-Triphenyltetrazolium chloride (SR0229K). Pour into sterile Petri dishes when using the membrane filtration method or hold at 45°C when using the pour-plate method.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Precautions
Observe the HAZARD precautions regarding sodium azide when disposing of the medium. The pH of the medium should not fall below 7.0 as it may become inhibitory towards enterococci. KF Streptococcus Agar is not specific for the presumptive identification of Group D streptococci. Further tests must be made to confirm the identity of the organisms isolated.
K30 Kanamycin
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Thermo ScientificTM OxoidTM
K5 Kanamycin
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Thermo ScientificTM OxoidTM
KAA Agar Base 500g
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Thermo ScientificTM OxoidTM
- Oxoid Kanamycin Aesculin Azide Agar Base is a selective medium for the isolation of Enterococci in foodstuffs.
- Also available Kanamycin Sulphate Selective Supplement, Part No. SR0092E.
Kanamycin Aesculin Azide Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 18.8 |
| Yeast extract | 5.0 |
| Sodium chloride | 5.0 |
| Sodium citrate | 1.0 |
| Aesculin | 1.0 |
| Ferric ammonium citrate | 0.5 |
| Sodium azide | 0.15 |
| Starch | 0.6 |
| Mix for Streptococci | 0.6 |
| Agar | 10.0 |
| Final pH 7.0 ± 0.2 @ 25°C |
Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Suspend 21.3g in 500ml of distilled water. Add one vial of Kanamycin Supplement (SR0092) reconstituted as directed. Bring to the boil. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.
Precautions
Observe the hazard precautions regarding sodium azide when disposing of the medium.
There is no universal medium which will isolate all strains of enterococci.
Unless a presumptive count is acceptable all isolates should have their identity confirmed with further tests.
Kanamycin Sulphate Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Kanamycin Sulphate Supplement (10mg/vial) is added to Kanamycin Esculin Azide Agar Base, Part No. CM0591B.
Kanamycin Sulphate Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Kanamycin sulphate | 10.0mg | 20.0mg |
Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Add 2ml of sterile distilled water to one vial and mix gently to dissolve completely. Add the contents to 500ml of Kanamycin Aesculin Azide Agar Base.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.
Karmaili Agar Base 500g
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Thermo ScientificTM OxoidTM
- Oxoid Campylobacter Karmaili Agar Base is a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.
- Also available Campylobacter Selective Supplement (Karmaili), Part No. SR0167E and Modified Karma-i-li Selective Supplement, Part No. SR0205E.
Campylobacter Karmaili Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Columbia Agar Base | 39.0 |
| Activated charcoal | 4.0 |
| Haemin | 0.032 |
| Final pH 7.4 ± 0.2 @ 25°C |
Campylobacter Agar (Karmali), Oxoid Preparation:
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.
Kf30 Cephalothin
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Thermo ScientificTM OxoidTM
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Cephalothin Antimicrobial Susceptibility Disks.
Kjeldahl Tablets for Wieninger Method, Merck
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Merck
Kjeldahl tablets for Wieninger method 5g/tablet.
Type: Wieninger catalyst Composition:
Na2SO4 – 96.5%
CuSO4 – 1.5%
Se – 2.0%
Kjeldahl tablets is grey colour in its appearance. Grey colour of the product is caused by raw material and does not affect the application.
Kligler Iron Agar 500g, Oxoid
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Thermo ScientificTM OxoidTM
Oxoid Kligler Iron Agar is used for differential identificaton of Enterobacteriaceae.
Kligler Iron Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| `Lab-Lemco’ powder | 3.0 |
| Yeast extract | 3.0 |
| Peptone | 20.0 |
| Sodium chloride | 5.0 |
| Lactose | 10.0 |
| Glucose | 1.0 |
| Ferric citrate | 0.3 |
| Sodium thiosulphate | 0.3 |
| Phenol red | 0.05 |
| Agar | 12.0 |
| pH 7.4 ± 0.2 @ 25°C |
Kligler Iron Agar, Oxoid Preparation:
Suspend 55g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set as slopes with 1 inch butts.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
It is essential that Kligler Iron Agar is examined and reported at 18-24 hours. Early or late readings will give false results.
KIA will grow both oxidative and fermentative organisms. Confusion will result if care is not taken to distinguish between the two groups.
Always use a straight wire to inoculate the butt, to avoid splitting the agar with a loop.
Pure cultures are essential to avoid misinterpretation.
Do not use screw-capped tubes or bottles for KIA medium. It is essential that air is freely available to growth on the slant.
Kz30 Cephazolin
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Thermo ScientificTM OxoidTM
L-Ornithine Monohydrochloride for Biochemistry (Merck)
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Merck
Description
CAS number: 3184-13-2
Linear Formula: C₅H₁₂N₂O₂ * ClH
Molecular Weight: 168.62
Synonym: L(+)-2,5-Diamino valeric acid hydrochloride
Application
L-Ornithine monohydrochloride for biochemistry.
L10 Jumbo Roll/Industrial Roll Wipers, WYPALL
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WYPALL
Jumbo Roll Wipers, also known as industrial roll wipers, are widely used in the laboratory for cleaning purpose. It is extremely easy to use when use with the stands.
