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Oxoid Muller-Kauffmann Tetrathionate Broth Base is selective enrichment medium for isolation of Salmonella spp. and suppression of Proteus species.

Muller-Kauffmann Tetrathionate Broth Base, Oxoid Composition

Muller-Kauffmann Tetrathionate Broth, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil. Cool below 45°C and add, just prior to use, 19ml of iodine solution and 9.5ml of a 0.1% brilliant green solution. Mix well and fill out into sterile tubes or flasks.

Dissolve the potassium iodide in approximately 5ml of distilled water, add the iodide and gently warm the solution to completely dissolve it. Make up the volume to 100ml with distilled water.

Add the brilliant green to the distilled water and shake to dissolve the dye. Heat the solution to 100°C for 30 minutes and shake from time to time whilst cooling, to ensure that the dye has completely dissolved. Store in a brown glass bottle or away from light.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not autoclave the base broth.
Add the iodine solution and brilliant green just prior to use.
The medium is not suitable for the growth of Salmonella typhi, Salmonella sendai, Salmonella pullorum and Salmonella gallinarum.

• Oxoid Mycoplasma Agar Base is used for the selective isolation of Mycoplasma pneumoniae.
• Also available Mycoplasma Supplement P, Part No. SR0060C and Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Agar Base, Oxoid Composition

Mycoplasma Agar, Oxoid Preparation:
Add 35.5g to 1 litre of distilled water. Boil to dissolve the agar and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions in the HAZARDS section.
Do not use thallous acetate media to isolate Ureaplasma urealyticum.
Mycoplasmas may be suspected if (1) typical morphology (2) no growth in media without serum (3) colonies are embedded below the agar surface.

• Oxoid Mycoplasma Broth Base is used for the selective isolation of Mycoplasma species.
• Also available Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Broth Base, Oxoid Composition

Mycoplasma Broth, Oxoid Preparation:
Dissolve 25.5g in 1 litre of distilled water. Mix well and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions stated under Hazards section..
Sub-culture the broth to agar as soon as any indicator added begins to change colour before the pH change destroys the organism.

Oxoid Mycoplasma Supplement G is for the isolation of mycoplasmas.

• Add to Mycoplasma Pneumoniae Agar, Part No. CM0401B or CM0403B
• Each vial supplements 80mL of medium

Mycoplasma Supplement G, Oxoid Composition
POISON – CONTAINS THALLIUM SALT

Mycoplasma Medium Preparation:
Prepare the sterile supplement by aseptically adding 20ml sterile distilled water to the vial and mix gently. Add 1ml of Oxoid Mycoplasma Base without supplements to each of ten small bottles. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set. Aseptically add 2ml of the reconstituted Supplement-P to each bottle containing agar.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.

Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.

MYP Agar, Oxoid Composition

MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.