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Oxoid RPF Supplement (Rabbit Plasma Fibrinogen) is used for the isolation and enumeration of coagulase positive staphylococci from food.

• Use only with Baird-Parker Agar Base, RPF, Part No. CM0961B
• Each vial supplements 100mL of medium

RPF Supplement (Rabbit Plasma Fibrinogen), Oxoid Composition

RPF Supplement Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90 ml of sterile Baird-Parker Agar Base (RPF) CM0961 cooled to 48°C. Mix well and use immediately.

Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

Enumerate heterotrophic bacteria from drinking water samples with Thermo Scientific™ Oxoid™ R2A Agar (Dehydrated). This nutritionally reduced medium was specially designed to enhance the recovery of stressed and chlorine damaged bacteria from treated water samples. Stressed or injured organisms during water treatment are unable to grow on high nutrient media since faster-growing organisms outgrow the former. Therefore the use of a low nutrient medium like R2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.

R2A Agar, Oxoid Composition

R2A Agar, Oxoid Preparation:
Suspend 18.1g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
R2A Agar should be stored tightly capped in the original container at 10-30°C. When stored as directed the medium will remain stable until the expiry date printed on the label.

The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Raka-Ray Agar Base is a medium for the isolation of lactic acid bacteria in beer and brewing processes.
• Requires supplementation with phenylethanol, sorbitan mono-oleate, and cycloheximide Part No. SR0222C.

Raka-Ray Agar Base, Oxoid Composition

Raka-Ray Agar, Oxoid Preparation:
Suspend 77.1g in 1 litre of distilled water. Add 10ml of sorbitan mono-oleate and 7ml of 0.1% Cycloheximide Solution (SR0222). Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 3g of phenylethanol. Pour into sterile Petri dishes or distribute into 4ml volumes held at 55°C if the overlay technique is to be used.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Although the concentration of cycloheximide in the medium is below toxic levels, precautions should be observed as detailed under HAZARDS section.

Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated). Rappaport- Vassiliadis Soya Peptone Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated), Oxoid Composition

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated), Oxoid Preparation:
Suspend 26.75g in 1 litre of distilled water and heat gently to dissolve. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
RVS Broth should not be used if Salmonella Typhi is suspected.
In order to achieve optimum recovery it is recommended that the enrichment broth is incubated at 42 ± 1°C.

Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Enrichment Broth (Dehydrated). Rappaport-Vassiliadis Enrichment Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

Rappaport-Vassiliadis Enrichment Broth (Dehydrated), Oxoid Composition

THIS MEDIUM IS VERY HYGROSCOPIC AND MUST BE PROTECTED FROM MOISTURE.
The quantities given for the formula as classically described made 1110ml of medium. They have been published this way in the Oxoid literature to coincide with the scientific literature.

The directions for reconstituting Oxoid Rappaport-Vassiliadis (RV) Enrichment Broth follow usual Oxoid practice and specify the weight needed for 1 litre of medium.

Rappaport-Vassiliadis Enrichment Broth (Dehydrated), Oxoid Preparation:
Add 30g (the equivalent weight of dehydrated medium per litre) to 1 litre of distilled water. Heat gently until dissolved completely. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
RV Broth should not be used if Salmonella typhi is suspected.
Note the difference in weight between the classical formula on the label and the reduced weight per litre, using anhydrous magnesium chloride.